Analysis of proteome changes in bone remodelling induced by implant retained overdentures / Ainin Sofia Mohamad Napi

Analysis of proteome changes in bone remodelling induced by implant retained overdentures / Ainin Sofia Mohamad Napi

Implant-retained overdentures (IRO) have been successful in providing satisfactory treatment for edentulous patients but in most cases, they contribute to bone resorption. The pressure induced by the IRO on the soft tissue mucosa may have mediated proteome changes that lead to this bone resorption....

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Main Author: Mohamad Napi, Ainin Sofia
Format: Thesis
Language:English
Published: 2019
Online Access:https://ir.uitm.edu.my/id/eprint/79407/1/79407.pdf
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spelling my-uitm-ir.794072023-09-08T02:59:58Z Analysis of proteome changes in bone remodelling induced by implant retained overdentures / Ainin Sofia Mohamad Napi 2019 Mohamad Napi, Ainin Sofia Implant-retained overdentures (IRO) have been successful in providing satisfactory treatment for edentulous patients but in most cases, they contribute to bone resorption. The pressure induced by the IRO on the soft tissue mucosa may have mediated proteome changes that lead to this bone resorption. Thus, this study has been carried out to identify the proteome changes associated with bone remodeling induced by IRO. Tissue specimens (n=22) and saliva samples (n=22) were collected from implant-retained overdentures and healthy patients before proteins being extracted and precipitated from the samples. This study employed two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption-ionization time of-flight mass spectrophotometry (Maldi-ToF/ToF MS) peptide mass fingerprinting for precise protein identification related to bone remodeling in IRO users. The protein expressions were correlated with the quantified bone resorption by the three dimensional imaging which was encountered by the IRO wearer. The selection of differentially expressed seven spots based on the ANOVA values (p < 0.05) and 1.5- fold changes from tissue samples between the subjects (n=l 1) and the control (n=l 1) by Progenesis Same Spot software (Nonlinear Dynamics, Durham, NC) was preceded to protein identification by Maldi ToFV ToF. Through this approach, several proteins were identified: 78 kDa glucose-regulated protein, Serum albumin, Lumican, Ig gamma-1 chain C region, Hemoglobin subunit beta and Heat shock protein beta-1. Among these, three proteins were known involved with bone remodeling based on previous literatures which were 78 kDa glucose-regulated protein, Lumican, and Heat shock protein beta-1. It was found that 78 kDa glucose-regulated protein and Lumican were up-regulated by 2.2-fold (p= 0.015) and 1.8-fold (p= 0.005) respectively in implant retained overdentures wearers compared to the control group, relating their roles in bone resorption. Meanwhile, Heat shock protein beta-1 was downregulated by 2-fold (p=0.029) in IRO group conveying its possible potential roles in the formation of bone. Based on the cellular localization and functions, these proteins' roles could be fitted into the bone remodeling cycle and significantly believed played their parts in the mechanism involved in bone resorption phenomenon associated with IRO. 2019 Thesis https://ir.uitm.edu.my/id/eprint/79407/ https://ir.uitm.edu.my/id/eprint/79407/1/79407.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Dentistry Ahmad, Rohana
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Ahmad, Rohana
description Implant-retained overdentures (IRO) have been successful in providing satisfactory treatment for edentulous patients but in most cases, they contribute to bone resorption. The pressure induced by the IRO on the soft tissue mucosa may have mediated proteome changes that lead to this bone resorption. Thus, this study has been carried out to identify the proteome changes associated with bone remodeling induced by IRO. Tissue specimens (n=22) and saliva samples (n=22) were collected from implant-retained overdentures and healthy patients before proteins being extracted and precipitated from the samples. This study employed two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption-ionization time of-flight mass spectrophotometry (Maldi-ToF/ToF MS) peptide mass fingerprinting for precise protein identification related to bone remodeling in IRO users. The protein expressions were correlated with the quantified bone resorption by the three dimensional imaging which was encountered by the IRO wearer. The selection of differentially expressed seven spots based on the ANOVA values (p < 0.05) and 1.5- fold changes from tissue samples between the subjects (n=l 1) and the control (n=l 1) by Progenesis Same Spot software (Nonlinear Dynamics, Durham, NC) was preceded to protein identification by Maldi ToFV ToF. Through this approach, several proteins were identified: 78 kDa glucose-regulated protein, Serum albumin, Lumican, Ig gamma-1 chain C region, Hemoglobin subunit beta and Heat shock protein beta-1. Among these, three proteins were known involved with bone remodeling based on previous literatures which were 78 kDa glucose-regulated protein, Lumican, and Heat shock protein beta-1. It was found that 78 kDa glucose-regulated protein and Lumican were up-regulated by 2.2-fold (p= 0.015) and 1.8-fold (p= 0.005) respectively in implant retained overdentures wearers compared to the control group, relating their roles in bone resorption. Meanwhile, Heat shock protein beta-1 was downregulated by 2-fold (p=0.029) in IRO group conveying its possible potential roles in the formation of bone. Based on the cellular localization and functions, these proteins' roles could be fitted into the bone remodeling cycle and significantly believed played their parts in the mechanism involved in bone resorption phenomenon associated with IRO.
format Thesis
qualification_level Master's degree
author Mohamad Napi, Ainin Sofia
spellingShingle Mohamad Napi, Ainin Sofia
Analysis of proteome changes in bone remodelling induced by implant retained overdentures / Ainin Sofia Mohamad Napi
author_facet Mohamad Napi, Ainin Sofia
author_sort Mohamad Napi, Ainin Sofia
title Analysis of proteome changes in bone remodelling induced by implant retained overdentures / Ainin Sofia Mohamad Napi
title_short Analysis of proteome changes in bone remodelling induced by implant retained overdentures / Ainin Sofia Mohamad Napi
title_full Analysis of proteome changes in bone remodelling induced by implant retained overdentures / Ainin Sofia Mohamad Napi
title_fullStr Analysis of proteome changes in bone remodelling induced by implant retained overdentures / Ainin Sofia Mohamad Napi
title_full_unstemmed Analysis of proteome changes in bone remodelling induced by implant retained overdentures / Ainin Sofia Mohamad Napi
title_sort analysis of proteome changes in bone remodelling induced by implant retained overdentures / ainin sofia mohamad napi
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Dentistry
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/79407/1/79407.pdf
_version_ 1783736280303009792

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