Detection of p53 gene mutation in oral squamous cell carcinoma patients using multiplex ligation-dependent probe amplification in Malaysian population / Fatimah Suriati Sulaiman

P53 gene mutations observed at about 50 to 60 % in oral squamous cell carcinoma (OSCC). Until now, most of the studies were focused on exon 5 to 8 of p53 gene instead of the whole exons and it becomes a trend for p53 gene mutation study. However, the site of p53 gene mutation is still in controversy...

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Main Author:	Sulaiman, Fatimah Suriati
Format:	Thesis
Language:	English
Published:	2017
Online Access:	https://ir.uitm.edu.my/id/eprint/81828/1/81828.pdf
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id	my-uitm-ir.81828
record_format	uketd_dc
spelling	my-uitm-ir.818282023-11-09T08:54:26Z Detection of p53 gene mutation in oral squamous cell carcinoma patients using multiplex ligation-dependent probe amplification in Malaysian population / Fatimah Suriati Sulaiman 2017 Sulaiman, Fatimah Suriati P53 gene mutations observed at about 50 to 60 % in oral squamous cell carcinoma (OSCC). Until now, most of the studies were focused on exon 5 to 8 of p53 gene instead of the whole exons and it becomes a trend for p53 gene mutation study. However, the site of p53 gene mutation is still in controversy as available techniques showed different results. More importantly, using the same technique the reports demonstrated inconsistence mutation sites. The objective of the present study is to examine the status of p53 gene mutation in all exons (1 to 11). Recently, Multiplex Ligation-dependent Probe Amplification (MLPA) technique has proven to be reliable and efficient for the detection of mutation. Therefore, present study used Multiplex Ligation-dependent Probe Amplification (MLPA) to examine p53 gene mutation from exons 1 to 11. DNA specimens from 58 OSCC patients and 10 healthy (controls) were used in this study. Our results demonstrated that 31% of OSCC patient have p53 gene mutation. Among them 56% of mutation occurred in exon 3, followed by exon 4 which was 50%. Our study strongly indicated that exons 3 and 4 could be reliable and positive markers of OSCC development and progression. To our knowledge, this study for the first time showed that exon 3 is the hot spot region for p53 gene mutation in OSCC. 2017 Thesis https://ir.uitm.edu.my/id/eprint/81828/ https://ir.uitm.edu.my/id/eprint/81828/1/81828.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Dentistry Jamil, Kazi Ahsan
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Jamil, Kazi Ahsan
description	P53 gene mutations observed at about 50 to 60 % in oral squamous cell carcinoma (OSCC). Until now, most of the studies were focused on exon 5 to 8 of p53 gene instead of the whole exons and it becomes a trend for p53 gene mutation study. However, the site of p53 gene mutation is still in controversy as available techniques showed different results. More importantly, using the same technique the reports demonstrated inconsistence mutation sites. The objective of the present study is to examine the status of p53 gene mutation in all exons (1 to 11). Recently, Multiplex Ligation-dependent Probe Amplification (MLPA) technique has proven to be reliable and efficient for the detection of mutation. Therefore, present study used Multiplex Ligation-dependent Probe Amplification (MLPA) to examine p53 gene mutation from exons 1 to 11. DNA specimens from 58 OSCC patients and 10 healthy (controls) were used in this study. Our results demonstrated that 31% of OSCC patient have p53 gene mutation. Among them 56% of mutation occurred in exon 3, followed by exon 4 which was 50%. Our study strongly indicated that exons 3 and 4 could be reliable and positive markers of OSCC development and progression. To our knowledge, this study for the first time showed that exon 3 is the hot spot region for p53 gene mutation in OSCC.
format	Thesis
qualification_level	Bachelor degree
author	Sulaiman, Fatimah Suriati
spellingShingle	Sulaiman, Fatimah Suriati Detection of p53 gene mutation in oral squamous cell carcinoma patients using multiplex ligation-dependent probe amplification in Malaysian population / Fatimah Suriati Sulaiman
author_facet	Sulaiman, Fatimah Suriati
author_sort	Sulaiman, Fatimah Suriati
title	Detection of p53 gene mutation in oral squamous cell carcinoma patients using multiplex ligation-dependent probe amplification in Malaysian population / Fatimah Suriati Sulaiman
title_short	Detection of p53 gene mutation in oral squamous cell carcinoma patients using multiplex ligation-dependent probe amplification in Malaysian population / Fatimah Suriati Sulaiman
title_full	Detection of p53 gene mutation in oral squamous cell carcinoma patients using multiplex ligation-dependent probe amplification in Malaysian population / Fatimah Suriati Sulaiman
title_fullStr	Detection of p53 gene mutation in oral squamous cell carcinoma patients using multiplex ligation-dependent probe amplification in Malaysian population / Fatimah Suriati Sulaiman
title_full_unstemmed	Detection of p53 gene mutation in oral squamous cell carcinoma patients using multiplex ligation-dependent probe amplification in Malaysian population / Fatimah Suriati Sulaiman
title_sort	detection of p53 gene mutation in oral squamous cell carcinoma patients using multiplex ligation-dependent probe amplification in malaysian population / fatimah suriati sulaiman
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Dentistry
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/81828/1/81828.pdf
_version_	1783736337921212416

Detection of p53 gene mutation in oral squamous cell carcinoma patients using multiplex ligation-dependent probe amplification in Malaysian population / Fatimah Suriati Sulaiman

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