Molecular mechanism of stevioside on tnf-α-induced insulin resistance in 3T3-L1 adipocytes by integration of metabolomics, protein and gene expression / Norhisham Haron

Molecular mechanism of stevioside on tnf-α-induced insulin resistance in 3T3-L1 adipocytes by integration of metabolomics, protein and gene expression / Norhisham Haron

The increasing prevalence of insulin resistance (IR) and T2DM provides compelling evidence for the identification of novel biomarkers, molecular targets and the development of effective drugs to prevent and treat the disease. Stevioside (SVS), the main constituent of Stevia rebaudiana Bertoni, has s...

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Main Author: Haron, Norhisham
Format: Thesis
Language:English
Published: 2023
Online Access:https://ir.uitm.edu.my/id/eprint/82140/1/82140.pdf
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spelling my-uitm-ir.821402024-01-15T08:35:51Z Molecular mechanism of stevioside on tnf-α-induced insulin resistance in 3T3-L1 adipocytes by integration of metabolomics, protein and gene expression / Norhisham Haron 2023 Haron, Norhisham The increasing prevalence of insulin resistance (IR) and T2DM provides compelling evidence for the identification of novel biomarkers, molecular targets and the development of effective drugs to prevent and treat the disease. Stevioside (SVS), the main constituent of Stevia rebaudiana Bertoni, has several therapeutic effects for metabolic syndrome, including lowering blood pressure, reducing blood glucose levels, potentiating insulin secretion and improving insulin sensitivity. However, the molecular basis underlying the antidiabetic effect of SVS on metabolic changes and pathways of insulin resistance is unknown. Therefore, the aim of this study was to investigate the metabolome response of SVS in a cell culture model of insulin resistance using metabolomics, protein and gene expression analyses. 3T3-L1 adipocyte cells were stimulated with 1.0 ng/mL TNF-α for insulin resistance and treated with SVS or rosiglitazone maleate (RM). The cell lysate was harvested and analysed by liquid chromatography-mass spectrometry-quadrapole time-of-flight analysis (LC/MSQTOF). 2023 Thesis https://ir.uitm.edu.my/id/eprint/82140/ https://ir.uitm.edu.my/id/eprint/82140/1/82140.pdf text en public phd doctoral Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Eshak, Zolkapli
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Eshak, Zolkapli
description The increasing prevalence of insulin resistance (IR) and T2DM provides compelling evidence for the identification of novel biomarkers, molecular targets and the development of effective drugs to prevent and treat the disease. Stevioside (SVS), the main constituent of Stevia rebaudiana Bertoni, has several therapeutic effects for metabolic syndrome, including lowering blood pressure, reducing blood glucose levels, potentiating insulin secretion and improving insulin sensitivity. However, the molecular basis underlying the antidiabetic effect of SVS on metabolic changes and pathways of insulin resistance is unknown. Therefore, the aim of this study was to investigate the metabolome response of SVS in a cell culture model of insulin resistance using metabolomics, protein and gene expression analyses. 3T3-L1 adipocyte cells were stimulated with 1.0 ng/mL TNF-α for insulin resistance and treated with SVS or rosiglitazone maleate (RM). The cell lysate was harvested and analysed by liquid chromatography-mass spectrometry-quadrapole time-of-flight analysis (LC/MSQTOF).
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Haron, Norhisham
spellingShingle Haron, Norhisham
Molecular mechanism of stevioside on tnf-α-induced insulin resistance in 3T3-L1 adipocytes by integration of metabolomics, protein and gene expression / Norhisham Haron
author_facet Haron, Norhisham
author_sort Haron, Norhisham
title Molecular mechanism of stevioside on tnf-α-induced insulin resistance in 3T3-L1 adipocytes by integration of metabolomics, protein and gene expression / Norhisham Haron
title_short Molecular mechanism of stevioside on tnf-α-induced insulin resistance in 3T3-L1 adipocytes by integration of metabolomics, protein and gene expression / Norhisham Haron
title_full Molecular mechanism of stevioside on tnf-α-induced insulin resistance in 3T3-L1 adipocytes by integration of metabolomics, protein and gene expression / Norhisham Haron
title_fullStr Molecular mechanism of stevioside on tnf-α-induced insulin resistance in 3T3-L1 adipocytes by integration of metabolomics, protein and gene expression / Norhisham Haron
title_full_unstemmed Molecular mechanism of stevioside on tnf-α-induced insulin resistance in 3T3-L1 adipocytes by integration of metabolomics, protein and gene expression / Norhisham Haron
title_sort molecular mechanism of stevioside on tnf-α-induced insulin resistance in 3t3-l1 adipocytes by integration of metabolomics, protein and gene expression / norhisham haron
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Pharmacy
publishDate 2023
url https://ir.uitm.edu.my/id/eprint/82140/1/82140.pdf
_version_ 1794191922860916736

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