In vitro melanogenesis, antioxidant and cytotoxicity activities of Peltophorum pterocarpum leaves extracts / Ana Sharmila Shafie

Melanin is a protective pigment against cellular damage and skin cancer. Expansion in demand for skin whitening treatments that lower melanin levels therefore have become a worrying trend as it may compromise the skin barrier. Peltophorum pterocarpum has been used for centuries to treat skin problem...

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Main Author:	Shafie, Ana Sharmila
Format:	Thesis
Language:	English
Published:	2023
Subjects:	Cytology Pathologic pigmentation
Online Access:	https://ir.uitm.edu.my/id/eprint/82577/1/82577.pdf
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id	my-uitm-ir.82577
record_format	uketd_dc
spelling	my-uitm-ir.825772024-01-15T08:48:01Z In vitro melanogenesis, antioxidant and cytotoxicity activities of Peltophorum pterocarpum leaves extracts / Ana Sharmila Shafie 2023 Shafie, Ana Sharmila Cytology Pathologic pigmentation Melanin is a protective pigment against cellular damage and skin cancer. Expansion in demand for skin whitening treatments that lower melanin levels therefore have become a worrying trend as it may compromise the skin barrier. Peltophorum pterocarpum has been used for centuries to treat skin problems due to its anti-oxidant and anti-microbial properties. However, the effects of P. pterocarpum extract on melanogenesis remain to be investigated. This study is aimed to examine the melanogenesis, anti-oxidant, and cytotoxicity activities of P. pterocarpum leaves extracts. A two-dimensional (2D) cell culture model was employed to demonstrate the efficacy of aqueous and ethanol of P. pterocarpum leaves extracts in promoting melanin synthesis. In vitro and cell-based detection of reactive oxygen species (ROS) were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) assays. The cytotoxicity effects of P. pterocarpum extracts were determined by [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] (MTT) and Neutral Red Uptake (NRU) assays on human keratinocyte cell lines and BALB/c 3T3 mouse fibroblasts, respectively. 2023 Thesis https://ir.uitm.edu.my/id/eprint/82577/ https://ir.uitm.edu.my/id/eprint/82577/1/82577.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Samsulrizal, Nurdiana
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Samsulrizal, Nurdiana
topic	Cytology Pathologic pigmentation
spellingShingle	Cytology Pathologic pigmentation Shafie, Ana Sharmila In vitro melanogenesis, antioxidant and cytotoxicity activities of Peltophorum pterocarpum leaves extracts / Ana Sharmila Shafie
description	Melanin is a protective pigment against cellular damage and skin cancer. Expansion in demand for skin whitening treatments that lower melanin levels therefore have become a worrying trend as it may compromise the skin barrier. Peltophorum pterocarpum has been used for centuries to treat skin problems due to its anti-oxidant and anti-microbial properties. However, the effects of P. pterocarpum extract on melanogenesis remain to be investigated. This study is aimed to examine the melanogenesis, anti-oxidant, and cytotoxicity activities of P. pterocarpum leaves extracts. A two-dimensional (2D) cell culture model was employed to demonstrate the efficacy of aqueous and ethanol of P. pterocarpum leaves extracts in promoting melanin synthesis. In vitro and cell-based detection of reactive oxygen species (ROS) were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) assays. The cytotoxicity effects of P. pterocarpum extracts were determined by [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] (MTT) and Neutral Red Uptake (NRU) assays on human keratinocyte cell lines and BALB/c 3T3 mouse fibroblasts, respectively.
format	Thesis
qualification_level	Master's degree
author	Shafie, Ana Sharmila
author_facet	Shafie, Ana Sharmila
author_sort	Shafie, Ana Sharmila
title	In vitro melanogenesis, antioxidant and cytotoxicity activities of Peltophorum pterocarpum leaves extracts / Ana Sharmila Shafie
title_short	In vitro melanogenesis, antioxidant and cytotoxicity activities of Peltophorum pterocarpum leaves extracts / Ana Sharmila Shafie
title_full	In vitro melanogenesis, antioxidant and cytotoxicity activities of Peltophorum pterocarpum leaves extracts / Ana Sharmila Shafie
title_fullStr	In vitro melanogenesis, antioxidant and cytotoxicity activities of Peltophorum pterocarpum leaves extracts / Ana Sharmila Shafie
title_full_unstemmed	In vitro melanogenesis, antioxidant and cytotoxicity activities of Peltophorum pterocarpum leaves extracts / Ana Sharmila Shafie
title_sort	in vitro melanogenesis, antioxidant and cytotoxicity activities of peltophorum pterocarpum leaves extracts / ana sharmila shafie
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2023
url	https://ir.uitm.edu.my/id/eprint/82577/1/82577.pdf
_version_	1794191934287249408

In vitro melanogenesis, antioxidant and cytotoxicity activities of Peltophorum pterocarpum leaves extracts / Ana Sharmila Shafie

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