DNA extraction from Gallus gallus domesticus / Nurul Fitri Aida Ismal

DNA extraction from Gallus gallus domesticus / Nurul Fitri Aida Ismal

Domestic chicken (Gallus gallus domesticus) is one of the most important species for mankind due to economic and cultural significance. The purpose of the study is to extract and to determine the DNA molecule size of 25 µg liver tissue of Gallus gallus domesticus (chicken). The chicken liver was ext...

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Main Author: Ismal, Nurul Fitri Aida
Format: Thesis
Language:English
Published: 2016
Subjects:
Extraction (Chemistry)
Online Access:https://ir.uitm.edu.my/id/eprint/83840/1/83840.PDF
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spelling my-uitm-ir.838402024-01-22T03:54:08Z DNA extraction from Gallus gallus domesticus / Nurul Fitri Aida Ismal 2016 Ismal, Nurul Fitri Aida Extraction (Chemistry) Domestic chicken (Gallus gallus domesticus) is one of the most important species for mankind due to economic and cultural significance. The purpose of the study is to extract and to determine the DNA molecule size of 25 µg liver tissue of Gallus gallus domesticus (chicken). The chicken liver was extracted using DNeasy Blood and Tissue kit that involved four major phase including lysis, washing, binding and elution in order to yield the DNA. By using gel electrophoresis, the sizes of DNA molecule can be determined by comparing with DNA marker. From the result, the visualization of extracted DNA was done on gel electrophoresis method under UV trans-illuminator by staining the DNA with a nucleic acid stain. By comparing to the DNA marker, the size of extracted genomic DNA from the liver of G. gallus domesticus is about 200 base pair (bp). The finding base pair in the G. gallus domesticus liver is successful and the extraction DNA ready to for PCR and other molecular biology applications without any additional purification. 2016 Thesis https://ir.uitm.edu.my/id/eprint/83840/ https://ir.uitm.edu.my/id/eprint/83840/1/83840.PDF text en public degree Universiti Teknologi MARA Pahang Faculty of Applied Sciences
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
topic Extraction (Chemistry)
spellingShingle Extraction (Chemistry)
Ismal, Nurul Fitri Aida
DNA extraction from Gallus gallus domesticus / Nurul Fitri Aida Ismal
description Domestic chicken (Gallus gallus domesticus) is one of the most important species for mankind due to economic and cultural significance. The purpose of the study is to extract and to determine the DNA molecule size of 25 µg liver tissue of Gallus gallus domesticus (chicken). The chicken liver was extracted using DNeasy Blood and Tissue kit that involved four major phase including lysis, washing, binding and elution in order to yield the DNA. By using gel electrophoresis, the sizes of DNA molecule can be determined by comparing with DNA marker. From the result, the visualization of extracted DNA was done on gel electrophoresis method under UV trans-illuminator by staining the DNA with a nucleic acid stain. By comparing to the DNA marker, the size of extracted genomic DNA from the liver of G. gallus domesticus is about 200 base pair (bp). The finding base pair in the G. gallus domesticus liver is successful and the extraction DNA ready to for PCR and other molecular biology applications without any additional purification.
format Thesis
qualification_level Bachelor degree
author Ismal, Nurul Fitri Aida
author_facet Ismal, Nurul Fitri Aida
author_sort Ismal, Nurul Fitri Aida
title DNA extraction from Gallus gallus domesticus / Nurul Fitri Aida Ismal
title_short DNA extraction from Gallus gallus domesticus / Nurul Fitri Aida Ismal
title_full DNA extraction from Gallus gallus domesticus / Nurul Fitri Aida Ismal
title_fullStr DNA extraction from Gallus gallus domesticus / Nurul Fitri Aida Ismal
title_full_unstemmed DNA extraction from Gallus gallus domesticus / Nurul Fitri Aida Ismal
title_sort dna extraction from gallus gallus domesticus / nurul fitri aida ismal
granting_institution Universiti Teknologi MARA Pahang
granting_department Faculty of Applied Sciences
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/83840/1/83840.PDF
_version_ 1794191974317686784

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