Detection of lipase gene of local soil bacteria isolated from domestic waste sites / Farhana Mohamad Izam Suhaimi

Lipases are important enzymes and known as most powerful biocatalyst in biotechnology industries. The aim of this study was to determine the lipase production from local soil isolates of domestic waste sites by using qualitative and quantitative methods, to identify the bacterial isolates that produ...

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Main Author: Mohamad Izam Suhaimi, Farhana
Format: Thesis
Language:English
Published: 2018
Online Access:https://ir.uitm.edu.my/id/eprint/84262/1/84262.pdf
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spelling my-uitm-ir.842622024-02-19T04:16:38Z Detection of lipase gene of local soil bacteria isolated from domestic waste sites / Farhana Mohamad Izam Suhaimi 2018 Mohamad Izam Suhaimi, Farhana Lipases are important enzymes and known as most powerful biocatalyst in biotechnology industries. The aim of this study was to determine the lipase production from local soil isolates of domestic waste sites by using qualitative and quantitative methods, to identify the bacterial isolates that produced lipase via molecular methods and to detect the lipase gene segment from the chosen isolate with the highest lipase production. Six isolated lipase producing bacteria were selected and screened for their lipase activity. All isolates were isolated from two local domestic waste site soils at Shah Alam, Selangor namely garbage site and night market site. 2018 Thesis https://ir.uitm.edu.my/id/eprint/84262/ https://ir.uitm.edu.my/id/eprint/84262/1/84262.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Mohd. Yusof, Farida Zuraina
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Mohd. Yusof, Farida Zuraina
description Lipases are important enzymes and known as most powerful biocatalyst in biotechnology industries. The aim of this study was to determine the lipase production from local soil isolates of domestic waste sites by using qualitative and quantitative methods, to identify the bacterial isolates that produced lipase via molecular methods and to detect the lipase gene segment from the chosen isolate with the highest lipase production. Six isolated lipase producing bacteria were selected and screened for their lipase activity. All isolates were isolated from two local domestic waste site soils at Shah Alam, Selangor namely garbage site and night market site.
format Thesis
qualification_level Master's degree
author Mohamad Izam Suhaimi, Farhana
spellingShingle Mohamad Izam Suhaimi, Farhana
Detection of lipase gene of local soil bacteria isolated from domestic waste sites / Farhana Mohamad Izam Suhaimi
author_facet Mohamad Izam Suhaimi, Farhana
author_sort Mohamad Izam Suhaimi, Farhana
title Detection of lipase gene of local soil bacteria isolated from domestic waste sites / Farhana Mohamad Izam Suhaimi
title_short Detection of lipase gene of local soil bacteria isolated from domestic waste sites / Farhana Mohamad Izam Suhaimi
title_full Detection of lipase gene of local soil bacteria isolated from domestic waste sites / Farhana Mohamad Izam Suhaimi
title_fullStr Detection of lipase gene of local soil bacteria isolated from domestic waste sites / Farhana Mohamad Izam Suhaimi
title_full_unstemmed Detection of lipase gene of local soil bacteria isolated from domestic waste sites / Farhana Mohamad Izam Suhaimi
title_sort detection of lipase gene of local soil bacteria isolated from domestic waste sites / farhana mohamad izam suhaimi
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Applied Sciences
publishDate 2018
url https://ir.uitm.edu.my/id/eprint/84262/1/84262.pdf
_version_ 1794191987445858304