Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi
Butt rot disease caused by Thielaviopsis paradoxa is an important disease of pineapple planting material in Malaysia. Plant extracts and antagonistic bacteria are potential biocontrol agents as they are effective and safer than chemical controls. In this study, five groups of antagonistic bacteria w...
Saved in:
Main Author: | |
---|---|
Format: | Thesis |
Language: | English |
Published: |
2018
|
Subjects: | |
Online Access: | https://ir.uitm.edu.my/id/eprint/84275/1/84275.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my-uitm-ir.84275 |
---|---|
record_format |
uketd_dc |
spelling |
my-uitm-ir.842752024-03-14T02:43:31Z Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi 2018 Mat Nawi, Nurnadirah QR Microbiology Butt rot disease caused by Thielaviopsis paradoxa is an important disease of pineapple planting material in Malaysia. Plant extracts and antagonistic bacteria are potential biocontrol agents as they are effective and safer than chemical controls. In this study, five groups of antagonistic bacteria were isolated from soil sampled from pineapple plants infected with butt rot disease and non-infected plants and four species of plant extracts, namely Leucaena leucocephala (leaf, bark) Eucalyptus camaldulensis (leaf), Alpinia galanga (rhizome) and Cinnamomum iners (bark) were tested against fungal pathogen. In vitro antifungal potential of both biocontrol agents were evaluated against T. paradoxa. Well diffusion method was used to evaluate antifungal activity of plant extracts, while dual culture method was used to test the antifungal activity of antagonistic bacteria. The result indicated that, among all the tested extracts, three ethanol extracts of L. leucocephala bark, E. camaldulensis leaf and A. galangdl rhizome and two chloroform extracts of L. leucocephala leaf and C. iners bark have showed their antifungal activities with 1.25%, 1.5%, 1.5%, 2.25% and 42.75% radial growth inhibition. Antagonistic bacteria, Bl showed the highest radial growth inhibition with 75%. Results also showed that B2, B3, B4 and B5 bacteria exhibited positive inhibition towards the pathogen with 70.75%, 57.25%, 56.25% and 47.50% radial growth inhibition, respectively. Microscopic observation of antifungal activities by plant extracts and antagonistic bacteria showed that the antifungal activities were via degradation, retardation, malformation, swelling, coagulation and breakage of the hyphae of T. paradoxa 2018 Thesis https://ir.uitm.edu.my/id/eprint/84275/ https://ir.uitm.edu.my/id/eprint/84275/1/84275.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Plantation and Agrotechnology Che Mohd Ramli, Neni Kartini |
institution |
Universiti Teknologi MARA |
collection |
UiTM Institutional Repository |
language |
English |
advisor |
Che Mohd Ramli, Neni Kartini |
topic |
QR Microbiology |
spellingShingle |
QR Microbiology Mat Nawi, Nurnadirah Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi |
description |
Butt rot disease caused by Thielaviopsis paradoxa is an important disease of pineapple planting material in Malaysia. Plant extracts and antagonistic bacteria are potential biocontrol agents as they are effective and safer than chemical controls. In this study, five groups of antagonistic bacteria were isolated from soil sampled from pineapple plants infected with butt rot disease and non-infected plants and four species of plant extracts, namely Leucaena leucocephala (leaf, bark) Eucalyptus camaldulensis (leaf), Alpinia galanga (rhizome) and Cinnamomum iners (bark) were tested against fungal pathogen. In vitro antifungal potential of both biocontrol agents were evaluated against T. paradoxa. Well diffusion method was used to evaluate antifungal activity of plant extracts, while dual culture method was used to test the antifungal activity of antagonistic bacteria. The result indicated that, among all the tested extracts, three ethanol extracts of L. leucocephala bark, E. camaldulensis leaf and A. galangdl rhizome and two chloroform extracts of L. leucocephala leaf and C. iners bark have showed their antifungal activities with 1.25%, 1.5%, 1.5%, 2.25% and 42.75% radial growth inhibition. Antagonistic bacteria, Bl showed the highest radial growth inhibition with 75%. Results also showed that B2, B3, B4 and B5 bacteria exhibited positive inhibition towards the pathogen with 70.75%, 57.25%, 56.25% and 47.50% radial growth inhibition, respectively. Microscopic observation of antifungal activities by plant extracts and antagonistic bacteria showed that the antifungal activities were via degradation, retardation, malformation, swelling, coagulation and breakage of the hyphae of T. paradoxa |
format |
Thesis |
qualification_level |
Master's degree |
author |
Mat Nawi, Nurnadirah |
author_facet |
Mat Nawi, Nurnadirah |
author_sort |
Mat Nawi, Nurnadirah |
title |
Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi |
title_short |
Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi |
title_full |
Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi |
title_fullStr |
Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi |
title_full_unstemmed |
Evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / Nurnadirah Mat Nawi |
title_sort |
evaluation of in vitro antifungal activity of selected plant extracts and antagonistic bacteria against butt rot disease of pineapple / nurnadirah mat nawi |
granting_institution |
Universiti Teknologi MARA (UiTM) |
granting_department |
Faculty of Plantation and Agrotechnology |
publishDate |
2018 |
url |
https://ir.uitm.edu.my/id/eprint/84275/1/84275.pdf |
_version_ |
1794191988651720704 |