Screening and characterisation of endogenous betaretrovirus in small mammals / Nur Fatin Aman Mohd Razlan Mahandran

Betaretrovirus is capable of crossing the barrier between human and other animals through cross-species transmission. Bats and guinea pigs have a great potential as a vector for virus infections a:s they possess ecological traits that enable them to spread viral diseases geographically. An investiga...

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Main Author:	Mohd Razlan Mahandran, Nur Fatin Aman
Format:	Thesis
Language:	English
Published:	2017
Subjects:	QR Microbiology
Online Access:	https://ir.uitm.edu.my/id/eprint/84299/1/84299.pdf
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id	my-uitm-ir.84299
record_format	uketd_dc
spelling	my-uitm-ir.842992024-04-19T09:31:52Z Screening and characterisation of endogenous betaretrovirus in small mammals / Nur Fatin Aman Mohd Razlan Mahandran 2017 Mohd Razlan Mahandran, Nur Fatin Aman QR Microbiology Betaretrovirus is capable of crossing the barrier between human and other animals through cross-species transmission. Bats and guinea pigs have a great potential as a vector for virus infections a:s they possess ecological traits that enable them to spread viral diseases geographically. An investigation was carried out using bioinformatic and molecular approaches to search for the presence of endogenous betaretrovirus in bats (PvEB) and guinea pigs (CpEB) genome. A total of 60 genome project databases were screened using BLAST and 46 tissue samples from small mammals were screened for pro;.rt gene using PCR amplification. Only 2 species were further investigated due to their significant BLAST result, namely flying (ox (Pteropus vampyrus) and guinea pig ( Cavia porcellus). Based on insilico characterisation work, a full-length genome of PvEB and CpEB was found with the size of 8772bp and. 8543bp respectively. Both had a typical genomic organisation of a betaretrovirus. Conserved motifs of gag,pol and env were further supported through Pfam-A analysis and protein structure prediction. Subsequently, phylogenetic tree analysis of gag, pol and full-length sequence had placed PvEB and CpEB robustly within betaretroviruses clade with strong .bootstrap support. Age estimation has revealed that each PvEB and CpEB had diverged from its host 8.8 and 47.lmya respectively. The results of bioinformatic findings were further verified through PCR amplification of pro-rt region in PvEB and CpEB.. Therefore, based on bioinformatics and molecular approaches, it is evident that PvEB and CpEB have significant characteristics of a type'."D betaretrovirus and have involved in cross-species transmission. 2017 Thesis https://ir.uitm.edu.my/id/eprint/84299/ https://ir.uitm.edu.my/id/eprint/84299/1/84299.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Kambol, Roziah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Kambol, Roziah
topic	QR Microbiology
spellingShingle	QR Microbiology Mohd Razlan Mahandran, Nur Fatin Aman Screening and characterisation of endogenous betaretrovirus in small mammals / Nur Fatin Aman Mohd Razlan Mahandran
description	Betaretrovirus is capable of crossing the barrier between human and other animals through cross-species transmission. Bats and guinea pigs have a great potential as a vector for virus infections a:s they possess ecological traits that enable them to spread viral diseases geographically. An investigation was carried out using bioinformatic and molecular approaches to search for the presence of endogenous betaretrovirus in bats (PvEB) and guinea pigs (CpEB) genome. A total of 60 genome project databases were screened using BLAST and 46 tissue samples from small mammals were screened for pro;.rt gene using PCR amplification. Only 2 species were further investigated due to their significant BLAST result, namely flying (ox (Pteropus vampyrus) and guinea pig ( Cavia porcellus). Based on insilico characterisation work, a full-length genome of PvEB and CpEB was found with the size of 8772bp and. 8543bp respectively. Both had a typical genomic organisation of a betaretrovirus. Conserved motifs of gag,pol and env were further supported through Pfam-A analysis and protein structure prediction. Subsequently, phylogenetic tree analysis of gag, pol and full-length sequence had placed PvEB and CpEB robustly within betaretroviruses clade with strong .bootstrap support. Age estimation has revealed that each PvEB and CpEB had diverged from its host 8.8 and 47.lmya respectively. The results of bioinformatic findings were further verified through PCR amplification of pro-rt region in PvEB and CpEB.. Therefore, based on bioinformatics and molecular approaches, it is evident that PvEB and CpEB have significant characteristics of a type'."D betaretrovirus and have involved in cross-species transmission.
format	Thesis
qualification_level	Master's degree
author	Mohd Razlan Mahandran, Nur Fatin Aman
author_facet	Mohd Razlan Mahandran, Nur Fatin Aman
author_sort	Mohd Razlan Mahandran, Nur Fatin Aman
title	Screening and characterisation of endogenous betaretrovirus in small mammals / Nur Fatin Aman Mohd Razlan Mahandran
title_short	Screening and characterisation of endogenous betaretrovirus in small mammals / Nur Fatin Aman Mohd Razlan Mahandran
title_full	Screening and characterisation of endogenous betaretrovirus in small mammals / Nur Fatin Aman Mohd Razlan Mahandran
title_fullStr	Screening and characterisation of endogenous betaretrovirus in small mammals / Nur Fatin Aman Mohd Razlan Mahandran
title_full_unstemmed	Screening and characterisation of endogenous betaretrovirus in small mammals / Nur Fatin Aman Mohd Razlan Mahandran
title_sort	screening and characterisation of endogenous betaretrovirus in small mammals / nur fatin aman mohd razlan mahandran
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/84299/1/84299.pdf
_version_	1804889719291510784

Screening and characterisation of endogenous betaretrovirus in small mammals / Nur Fatin Aman Mohd Razlan Mahandran

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