Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir

This study aims to evaluate the potential of selected bacteria in caffeine and carbamazepine degradation. The objectives of this study are to screen potential bacteria in degradation of caffeine and carbamazepine, to optimise degradation parameters of caffeine and carbamazepine as well as to evaluat...

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Main Author:	Mohamad Nasir, Najihah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	TD Environmental technology Sanitary engineering
Online Access:	https://ir.uitm.edu.my/id/eprint/84335/1/84335.pdf
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id	my-uitm-ir.84335
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spelling	my-uitm-ir.843352024-07-24T03:58:39Z Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir 2019 Mohamad Nasir, Najihah TD Environmental technology. Sanitary engineering This study aims to evaluate the potential of selected bacteria in caffeine and carbamazepine degradation. The objectives of this study are to screen potential bacteria in degradation of caffeine and carbamazepine, to optimise degradation parameters of caffeine and carbamazepine as well as to evaluate degrading enzymes for prediction of degradation pathways. Methodologies included screening of bacteria resistance towards caffeine and carbamazepine and optimisation parameters of initial concentrations, initial pH and temperature for degradation of caffeine and carbamazepine. Then, mixed culture study was conducted at the selected optimum condition. At the same time, bacteria growth for Rhodococcus zopfii and Leifsonia shinshuensis was determined to support the degradation result. In addition, enzyme assays for oxygen oxidoreductase, aldehyde oxidase, catechol 1,2 dioxygenase and catechol 2,3 dioxygenase were also conducted. The result demonstrated that bacteria R. zopfii and L. shinshuensis were selected due to growth and potential in utilising caffeine and carbamazepine as nutrient source for degradation. For single culture degradation study, the 10 mgL-1, pH 7 and 40 °C were selected as optimum condition for degradation of caffeine and carbamazepine. Optimum normalised degradation percentage of caffeine by R. zopfii was 72.53 ± 0.10 and L. shinshuensis was only 38.49 ± 0.01. For carbamazepine, both R. zopfii and L.shinshuensis achieved high normalised degradation percentage of 99.99 ± 0.00. Degradation of caffeine was not correlated to bacteria growth meanwhile, degradation of carbamazepine was linearly correlated to the bacteria growth. The result also revealed antagonistic interaction between R. zopfii and L. shinshuensis in mixed culture. Furthermore, enzyme assay for bacteria in caffeine showed presence of enzymes oxygen oxidoreductase and aldehyde oxidase, meanwhile, oxygen oxidoreductase, aldehyde oxidase, catechol 1,2 dioxygenase and catechol 2,3 dioxygenase were determined during carbamazepine degradation. 2019 Thesis https://ir.uitm.edu.my/id/eprint/84335/ https://ir.uitm.edu.my/id/eprint/84335/1/84335.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Tay, Chia Chay
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Tay, Chia Chay
topic	TD Environmental technology Sanitary engineering
spellingShingle	TD Environmental technology Sanitary engineering Mohamad Nasir, Najihah Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
description	This study aims to evaluate the potential of selected bacteria in caffeine and carbamazepine degradation. The objectives of this study are to screen potential bacteria in degradation of caffeine and carbamazepine, to optimise degradation parameters of caffeine and carbamazepine as well as to evaluate degrading enzymes for prediction of degradation pathways. Methodologies included screening of bacteria resistance towards caffeine and carbamazepine and optimisation parameters of initial concentrations, initial pH and temperature for degradation of caffeine and carbamazepine. Then, mixed culture study was conducted at the selected optimum condition. At the same time, bacteria growth for Rhodococcus zopfii and Leifsonia shinshuensis was determined to support the degradation result. In addition, enzyme assays for oxygen oxidoreductase, aldehyde oxidase, catechol 1,2 dioxygenase and catechol 2,3 dioxygenase were also conducted. The result demonstrated that bacteria R. zopfii and L. shinshuensis were selected due to growth and potential in utilising caffeine and carbamazepine as nutrient source for degradation. For single culture degradation study, the 10 mgL-1, pH 7 and 40 °C were selected as optimum condition for degradation of caffeine and carbamazepine. Optimum normalised degradation percentage of caffeine by R. zopfii was 72.53 ± 0.10 and L. shinshuensis was only 38.49 ± 0.01. For carbamazepine, both R. zopfii and L.shinshuensis achieved high normalised degradation percentage of 99.99 ± 0.00. Degradation of caffeine was not correlated to bacteria growth meanwhile, degradation of carbamazepine was linearly correlated to the bacteria growth. The result also revealed antagonistic interaction between R. zopfii and L. shinshuensis in mixed culture. Furthermore, enzyme assay for bacteria in caffeine showed presence of enzymes oxygen oxidoreductase and aldehyde oxidase, meanwhile, oxygen oxidoreductase, aldehyde oxidase, catechol 1,2 dioxygenase and catechol 2,3 dioxygenase were determined during carbamazepine degradation.
format	Thesis
qualification_level	Master's degree
author	Mohamad Nasir, Najihah
author_facet	Mohamad Nasir, Najihah
author_sort	Mohamad Nasir, Najihah
title	Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
title_short	Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
title_full	Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
title_fullStr	Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
title_full_unstemmed	Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
title_sort	biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / najihah mohamad nasir
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/84335/1/84335.pdf
_version_	1811768699894366208

Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir

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