The impact of bisphenol an exposure in utero and postnatal trans-fat diet on small intestine global DNA methylation and gut microbiome of sprague dawley rats / Sarah Zulkifli

The impact of bisphenol an exposure in utero and postnatal trans-fat diet on small intestine global DNA methylation and gut microbiome of sprague dawley rats / Sarah Zulkifli

A multitude of evidence has pointed out the repercussions of prenatal bisphenol A (BPA) exposure with a postnatal high fat diet on offspring’s health. However, the information on how the interaction between these two variables affects the small intestinal cells and the gut microbiome is rather limit...

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Main Author: Zulkifli, Sarah
Format: Thesis
Language:English
Published: 2023
Online Access:https://ir.uitm.edu.my/id/eprint/88722/1/88722.pdf
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spelling my-uitm-ir.887222024-01-17T00:40:56Z The impact of bisphenol an exposure in utero and postnatal trans-fat diet on small intestine global DNA methylation and gut microbiome of sprague dawley rats / Sarah Zulkifli 2023 Zulkifli, Sarah A multitude of evidence has pointed out the repercussions of prenatal bisphenol A (BPA) exposure with a postnatal high fat diet on offspring’s health. However, the information on how the interaction between these two variables affects the small intestinal cells and the gut microbiome is rather limited. Hence, we investigated the impact of postnatal trans fat diet (TFD) on the small intestinal morphology and global DNA methylation, as well as the gut microbiome of offspring exposed and non-exposed to prenatal BPA. Eighteen pregnant rats were administered with either unspiked control (CTL), vehicle control (P80) or BPA drinking water throughout pregnancy. Twelve weaned pups from each pregnancy group were given either normal diet (ND) or TFD from the postnatal week (PNW) 3 till PNW14; divided into CTLND, CTLTFD, P80ND, P80TFD, BPAND, and BPATFD groups. Body weight (BW), waist circumference (WC), food and water intake were measured weekly in offspring. At PNW14, small intestines were collected for global DNA methylation and histological analyses. 2023 Thesis https://ir.uitm.edu.my/id/eprint/88722/ https://ir.uitm.edu.my/id/eprint/88722/1/88722.pdf text en public phd doctoral Universiti Teknologi MARA (UiTM) Faculty of Medicine Mohd Nor, Noor Shafina
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Mohd Nor, Noor Shafina
description A multitude of evidence has pointed out the repercussions of prenatal bisphenol A (BPA) exposure with a postnatal high fat diet on offspring’s health. However, the information on how the interaction between these two variables affects the small intestinal cells and the gut microbiome is rather limited. Hence, we investigated the impact of postnatal trans fat diet (TFD) on the small intestinal morphology and global DNA methylation, as well as the gut microbiome of offspring exposed and non-exposed to prenatal BPA. Eighteen pregnant rats were administered with either unspiked control (CTL), vehicle control (P80) or BPA drinking water throughout pregnancy. Twelve weaned pups from each pregnancy group were given either normal diet (ND) or TFD from the postnatal week (PNW) 3 till PNW14; divided into CTLND, CTLTFD, P80ND, P80TFD, BPAND, and BPATFD groups. Body weight (BW), waist circumference (WC), food and water intake were measured weekly in offspring. At PNW14, small intestines were collected for global DNA methylation and histological analyses.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Zulkifli, Sarah
spellingShingle Zulkifli, Sarah
The impact of bisphenol an exposure in utero and postnatal trans-fat diet on small intestine global DNA methylation and gut microbiome of sprague dawley rats / Sarah Zulkifli
author_facet Zulkifli, Sarah
author_sort Zulkifli, Sarah
title The impact of bisphenol an exposure in utero and postnatal trans-fat diet on small intestine global DNA methylation and gut microbiome of sprague dawley rats / Sarah Zulkifli
title_short The impact of bisphenol an exposure in utero and postnatal trans-fat diet on small intestine global DNA methylation and gut microbiome of sprague dawley rats / Sarah Zulkifli
title_full The impact of bisphenol an exposure in utero and postnatal trans-fat diet on small intestine global DNA methylation and gut microbiome of sprague dawley rats / Sarah Zulkifli
title_fullStr The impact of bisphenol an exposure in utero and postnatal trans-fat diet on small intestine global DNA methylation and gut microbiome of sprague dawley rats / Sarah Zulkifli
title_full_unstemmed The impact of bisphenol an exposure in utero and postnatal trans-fat diet on small intestine global DNA methylation and gut microbiome of sprague dawley rats / Sarah Zulkifli
title_sort impact of bisphenol an exposure in utero and postnatal trans-fat diet on small intestine global dna methylation and gut microbiome of sprague dawley rats / sarah zulkifli
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Medicine
publishDate 2023
url https://ir.uitm.edu.my/id/eprint/88722/1/88722.pdf
_version_ 1794192147366281216

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