Chemical constituents isolated from Pereskia bleo extracts and their antioxidant and antibacterial activities / Mas Athira Johari

Pereskia bleo is a popular medicinal plant used traditionally to treat cancer-related diseases, hypertension, diabetes, and various illnesses. Previous studies reported various interesting biological activities of different Pereskia species, especially cytotoxic activity. Based on ethnobotany uses o...

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Main Author:	Johari, Mas Athira
Format:	Thesis
Language:	English
Published:	2023
Subjects:	QK Botany
Online Access:	https://ir.uitm.edu.my/id/eprint/88750/1/88750.pdf
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spelling	my-uitm-ir.887502024-07-22T07:47:03Z Chemical constituents isolated from Pereskia bleo extracts and their antioxidant and antibacterial activities / Mas Athira Johari 2023 Johari, Mas Athira QK Botany Pereskia bleo is a popular medicinal plant used traditionally to treat cancer-related diseases, hypertension, diabetes, and various illnesses. Previous studies reported various interesting biological activities of different Pereskia species, especially cytotoxic activity. Based on ethnobotany uses of Pereskia species and some earlier data on its biological activities, there should be a unique structure of biologically active compounds that is responsible for its popularity and results that have been reported. Several known compounds have been isolated from previous studies. However, only one flavonoid, Vitexin, was reported in 2012. More work must be carried out on the isolation of pure compounds from this genus. This study aimed to isolate chemical compounds and elucidate their chemical structures using modern spectroscopic methods such as Liquid chromatography-mass spectroscopy (LC-MS), Nuclear magnetic resonance (NMR) spectroscopy, and Infrared (IR) spectroscopy. In addition, selected pure compounds and different solvent extracts were used to determine the antibacterial and antioxidant activities based on 1,2-diphynyl-2pricrylhydrazyl (DPPH), and minimal inhibitory concentrations (MIC) and minimum bacterial concentration (MBC) assays, respectively. In this study, four types of microorganisms were tested, namely Escherichia coli ATCC 10535 (EC), Pseudomonas aeruginos ATCC 9027 (PA), Staphylococcus aureus ATCC 29737 (SA), and Streptococcus pyogenes ATCC 19615 (SP). For the phytochemical study on the stem extract, one new chalcone derivative, 4-Hydroxy-2,3,6-trimethoxy chalcone (PB 2) and one known fatty acid, (Z)-16-methoxy-16-oxohexadec-7-enoic (PB 1) were elucidated through the extensive use of 1D and 2D NMR as well as LCMS. Two known phytosterols identified as beta-sitosterol (PB 3) and stigmasterol (PB 5) and one flavone derivative, namely 5,7dihydroxy-6-methoxy flavone (Oroxylin A) (PB 6), were found in the acetone stem extract. Besides, beta-sitosterol (PB 4) was also isolated from the hexane leaves extract. The methanolic leaf crude extract showed the most potent free radical scavenging activity, while all stem crude extracts showed intermediate antioxidant activity. For antibacterial activity, all chloroform and methanol extracts from its leaves and stem and acetone stem extract showed strong antibacterial activity against all tested bacteria. The antibacterial activity could be contributed by PB 2, PB 5, and PB 6, where the MBC value is less than 500 µg/mL. The result of this study is a significant contribution toward further understanding the chemotaxonomy of this genus and the Cactaceae family. The four bacteria used in this study are all related to food spoilage and food-borne diseases. Therefore, this study suggests that certain P. bleo could be used as a natural preservative in food to replace chemical preservatives. 2023 Thesis https://ir.uitm.edu.my/id/eprint/88750/ https://ir.uitm.edu.my/id/eprint/88750/1/88750.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Khong, Heng Yen
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Khong, Heng Yen
topic	QK Botany
spellingShingle	QK Botany Johari, Mas Athira Chemical constituents isolated from Pereskia bleo extracts and their antioxidant and antibacterial activities / Mas Athira Johari
description	Pereskia bleo is a popular medicinal plant used traditionally to treat cancer-related diseases, hypertension, diabetes, and various illnesses. Previous studies reported various interesting biological activities of different Pereskia species, especially cytotoxic activity. Based on ethnobotany uses of Pereskia species and some earlier data on its biological activities, there should be a unique structure of biologically active compounds that is responsible for its popularity and results that have been reported. Several known compounds have been isolated from previous studies. However, only one flavonoid, Vitexin, was reported in 2012. More work must be carried out on the isolation of pure compounds from this genus. This study aimed to isolate chemical compounds and elucidate their chemical structures using modern spectroscopic methods such as Liquid chromatography-mass spectroscopy (LC-MS), Nuclear magnetic resonance (NMR) spectroscopy, and Infrared (IR) spectroscopy. In addition, selected pure compounds and different solvent extracts were used to determine the antibacterial and antioxidant activities based on 1,2-diphynyl-2pricrylhydrazyl (DPPH), and minimal inhibitory concentrations (MIC) and minimum bacterial concentration (MBC) assays, respectively. In this study, four types of microorganisms were tested, namely Escherichia coli ATCC 10535 (EC), Pseudomonas aeruginos ATCC 9027 (PA), Staphylococcus aureus ATCC 29737 (SA), and Streptococcus pyogenes ATCC 19615 (SP). For the phytochemical study on the stem extract, one new chalcone derivative, 4-Hydroxy-2,3,6-trimethoxy chalcone (PB 2) and one known fatty acid, (Z)-16-methoxy-16-oxohexadec-7-enoic (PB 1) were elucidated through the extensive use of 1D and 2D NMR as well as LCMS. Two known phytosterols identified as beta-sitosterol (PB 3) and stigmasterol (PB 5) and one flavone derivative, namely 5,7dihydroxy-6-methoxy flavone (Oroxylin A) (PB 6), were found in the acetone stem extract. Besides, beta-sitosterol (PB 4) was also isolated from the hexane leaves extract. The methanolic leaf crude extract showed the most potent free radical scavenging activity, while all stem crude extracts showed intermediate antioxidant activity. For antibacterial activity, all chloroform and methanol extracts from its leaves and stem and acetone stem extract showed strong antibacterial activity against all tested bacteria. The antibacterial activity could be contributed by PB 2, PB 5, and PB 6, where the MBC value is less than 500 µg/mL. The result of this study is a significant contribution toward further understanding the chemotaxonomy of this genus and the Cactaceae family. The four bacteria used in this study are all related to food spoilage and food-borne diseases. Therefore, this study suggests that certain P. bleo could be used as a natural preservative in food to replace chemical preservatives.
format	Thesis
qualification_level	Master's degree
author	Johari, Mas Athira
author_facet	Johari, Mas Athira
author_sort	Johari, Mas Athira
title	Chemical constituents isolated from Pereskia bleo extracts and their antioxidant and antibacterial activities / Mas Athira Johari
title_short	Chemical constituents isolated from Pereskia bleo extracts and their antioxidant and antibacterial activities / Mas Athira Johari
title_full	Chemical constituents isolated from Pereskia bleo extracts and their antioxidant and antibacterial activities / Mas Athira Johari
title_fullStr	Chemical constituents isolated from Pereskia bleo extracts and their antioxidant and antibacterial activities / Mas Athira Johari
title_full_unstemmed	Chemical constituents isolated from Pereskia bleo extracts and their antioxidant and antibacterial activities / Mas Athira Johari
title_sort	chemical constituents isolated from pereskia bleo extracts and their antioxidant and antibacterial activities / mas athira johari
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2023
url	https://ir.uitm.edu.my/id/eprint/88750/1/88750.pdf
_version_	1811768728808849408

Chemical constituents isolated from Pereskia bleo extracts and their antioxidant and antibacterial activities / Mas Athira Johari

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