Genomic epidemiology of sporadic human Brucella melitensis cases in Malaysia through whole genome analysis / Jama’ayah Mohamed Zahidi @ Ahmad Bakri

Brucella melitensis is the most pathogenic and genetically diverged species within the genus Brucella. In Malaysia, human infections by B. melitensis are reported yearly and the cases are primarily sporadic. Whole genome sequencing (WGS) analysis of B. melitensis strains can provide high-quality inf...

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Main Author:	Mohamed Zahidi @ Ahmad Bakri, Jama’ayah
Format:	Thesis
Language:	English
Published:	2023
Online Access:	https://ir.uitm.edu.my/id/eprint/88773/1/88773.pdf
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id	my-uitm-ir.88773
record_format	uketd_dc
spelling	my-uitm-ir.887732023-12-26T07:48:21Z Genomic epidemiology of sporadic human Brucella melitensis cases in Malaysia through whole genome analysis / Jama’ayah Mohamed Zahidi @ Ahmad Bakri 2023 Mohamed Zahidi @ Ahmad Bakri, Jama’ayah Brucella melitensis is the most pathogenic and genetically diverged species within the genus Brucella. In Malaysia, human infections by B. melitensis are reported yearly and the cases are primarily sporadic. Whole genome sequencing (WGS) analysis of B. melitensis strains can provide high-quality information for genotyping, monitoring and trace-back purposes in epidemiological studies. This research aim is to determine the genetic relatedness of local B. melitensis strains compared to strains from other geographical locations and to study the genetic variation of these local B. melitensis strains isolated from sporadic human infections. In this study, whole genomic analysis of 25 sporadic human B. melitensis cases in Malaysia and 75 genome sequences of B. melitensis retrieved from the NCBI database. From a total of 100 genome sequences, thirty-four Malaysian strains (25 sporadic isolates in this study, six human isolates from other studies and three animal isolates) can be grouped into genotype II and segregated into a distinct lineage clade of South-East Asian (SEA). Phylogenetics analysis showed that 24 of these 25 strains clustered within a single clade supported by 95% bootstrap. One of the isolate was clustered closely with the Indian isolates strain Bm IND1. 2023 Thesis https://ir.uitm.edu.my/id/eprint/88773/ https://ir.uitm.edu.my/id/eprint/88773/1/88773.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Abdullah, Mohd Faiz Foong
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abdullah, Mohd Faiz Foong
description	Brucella melitensis is the most pathogenic and genetically diverged species within the genus Brucella. In Malaysia, human infections by B. melitensis are reported yearly and the cases are primarily sporadic. Whole genome sequencing (WGS) analysis of B. melitensis strains can provide high-quality information for genotyping, monitoring and trace-back purposes in epidemiological studies. This research aim is to determine the genetic relatedness of local B. melitensis strains compared to strains from other geographical locations and to study the genetic variation of these local B. melitensis strains isolated from sporadic human infections. In this study, whole genomic analysis of 25 sporadic human B. melitensis cases in Malaysia and 75 genome sequences of B. melitensis retrieved from the NCBI database. From a total of 100 genome sequences, thirty-four Malaysian strains (25 sporadic isolates in this study, six human isolates from other studies and three animal isolates) can be grouped into genotype II and segregated into a distinct lineage clade of South-East Asian (SEA). Phylogenetics analysis showed that 24 of these 25 strains clustered within a single clade supported by 95% bootstrap. One of the isolate was clustered closely with the Indian isolates strain Bm IND1.
format	Thesis
qualification_level	Master's degree
author	Mohamed Zahidi @ Ahmad Bakri, Jama’ayah
spellingShingle	Mohamed Zahidi @ Ahmad Bakri, Jama’ayah Genomic epidemiology of sporadic human Brucella melitensis cases in Malaysia through whole genome analysis / Jama’ayah Mohamed Zahidi @ Ahmad Bakri
author_facet	Mohamed Zahidi @ Ahmad Bakri, Jama’ayah
author_sort	Mohamed Zahidi @ Ahmad Bakri, Jama’ayah
title	Genomic epidemiology of sporadic human Brucella melitensis cases in Malaysia through whole genome analysis / Jama’ayah Mohamed Zahidi @ Ahmad Bakri
title_short	Genomic epidemiology of sporadic human Brucella melitensis cases in Malaysia through whole genome analysis / Jama’ayah Mohamed Zahidi @ Ahmad Bakri
title_full	Genomic epidemiology of sporadic human Brucella melitensis cases in Malaysia through whole genome analysis / Jama’ayah Mohamed Zahidi @ Ahmad Bakri
title_fullStr	Genomic epidemiology of sporadic human Brucella melitensis cases in Malaysia through whole genome analysis / Jama’ayah Mohamed Zahidi @ Ahmad Bakri
title_full_unstemmed	Genomic epidemiology of sporadic human Brucella melitensis cases in Malaysia through whole genome analysis / Jama’ayah Mohamed Zahidi @ Ahmad Bakri
title_sort	genomic epidemiology of sporadic human brucella melitensis cases in malaysia through whole genome analysis / jama’ayah mohamed zahidi @ ahmad bakri
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2023
url	https://ir.uitm.edu.my/id/eprint/88773/1/88773.pdf
_version_	1794192158683561984

Genomic epidemiology of sporadic human Brucella melitensis cases in Malaysia through whole genome analysis / Jama’ayah Mohamed Zahidi @ Ahmad Bakri

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