Proteomic characterization of Corynebacterium pseudotuberculosis biofilm / Syaida Anati Abd Rashid

Corynebacterium pseudotuberculosis is a pathogenic intracellular parasite causing caseous lymphadenitis (CLA) mostly in sheep and goats, thus, leading to significant economic losses. The morphology, biochemical composition, and antimicrobial susceptibility pattern of C. pseudotuberculosis biofilm ha...

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Main Author:	Abd Rashid, Syaida Anati
Format:	Thesis
Language:	English
Published:	2023
Online Access:	https://ir.uitm.edu.my/id/eprint/88941/1/88941.pdf
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id	my-uitm-ir.88941
record_format	uketd_dc
spelling	my-uitm-ir.889412024-01-03T06:38:10Z Proteomic characterization of Corynebacterium pseudotuberculosis biofilm / Syaida Anati Abd Rashid 2023 Abd Rashid, Syaida Anati Corynebacterium pseudotuberculosis is a pathogenic intracellular parasite causing caseous lymphadenitis (CLA) mostly in sheep and goats, thus, leading to significant economic losses. The morphology, biochemical composition, and antimicrobial susceptibility pattern of C. pseudotuberculosis biofilm have previously been addressed. However, little is known about the whole-cell proteome expression in C. pseudotuberculosis biofilm. The present study was performed to determine proteome profiles of planktonic and biofilm fractions of C. pseudotuberculosis using 1D-SDSPAGE and to identify differentially expressed proteins during the biofilm formation by C. pseudotuberculosis using LC-MS/MS. The FESEM image showed that heterogenous C. pseudotuberculosis biofilm was successfully formed within 24h. The treatment with antimicrobials substantially inhibited the viability of the C. pseudotuberculosis biofilm. The percentage of biofilm inhibition was found to be in the range between 12.92% to 87.44%. Both planktonic and biofilm fractions showed the expression of nine protein bands ranging between 33.7 kDa and 150 kDa. However, a protein band of 48.3 kDa appeared in the planktonic fraction but not in the biofilm fraction. 2023 Thesis https://ir.uitm.edu.my/id/eprint/88941/ https://ir.uitm.edu.my/id/eprint/88941/1/88941.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Raja Yahya, Mohd Fakharul Zaman
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Raja Yahya, Mohd Fakharul Zaman
description	Corynebacterium pseudotuberculosis is a pathogenic intracellular parasite causing caseous lymphadenitis (CLA) mostly in sheep and goats, thus, leading to significant economic losses. The morphology, biochemical composition, and antimicrobial susceptibility pattern of C. pseudotuberculosis biofilm have previously been addressed. However, little is known about the whole-cell proteome expression in C. pseudotuberculosis biofilm. The present study was performed to determine proteome profiles of planktonic and biofilm fractions of C. pseudotuberculosis using 1D-SDSPAGE and to identify differentially expressed proteins during the biofilm formation by C. pseudotuberculosis using LC-MS/MS. The FESEM image showed that heterogenous C. pseudotuberculosis biofilm was successfully formed within 24h. The treatment with antimicrobials substantially inhibited the viability of the C. pseudotuberculosis biofilm. The percentage of biofilm inhibition was found to be in the range between 12.92% to 87.44%. Both planktonic and biofilm fractions showed the expression of nine protein bands ranging between 33.7 kDa and 150 kDa. However, a protein band of 48.3 kDa appeared in the planktonic fraction but not in the biofilm fraction.
format	Thesis
qualification_level	Master's degree
author	Abd Rashid, Syaida Anati
spellingShingle	Abd Rashid, Syaida Anati Proteomic characterization of Corynebacterium pseudotuberculosis biofilm / Syaida Anati Abd Rashid
author_facet	Abd Rashid, Syaida Anati
author_sort	Abd Rashid, Syaida Anati
title	Proteomic characterization of Corynebacterium pseudotuberculosis biofilm / Syaida Anati Abd Rashid
title_short	Proteomic characterization of Corynebacterium pseudotuberculosis biofilm / Syaida Anati Abd Rashid
title_full	Proteomic characterization of Corynebacterium pseudotuberculosis biofilm / Syaida Anati Abd Rashid
title_fullStr	Proteomic characterization of Corynebacterium pseudotuberculosis biofilm / Syaida Anati Abd Rashid
title_full_unstemmed	Proteomic characterization of Corynebacterium pseudotuberculosis biofilm / Syaida Anati Abd Rashid
title_sort	proteomic characterization of corynebacterium pseudotuberculosis biofilm / syaida anati abd rashid
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2023
url	https://ir.uitm.edu.my/id/eprint/88941/1/88941.pdf
_version_	1794192178575048704

Proteomic characterization of Corynebacterium pseudotuberculosis biofilm / Syaida Anati Abd Rashid

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