Screening method for initiation and maturation of somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 / Siti Hidayatul Umairah Bahrom

The establishment of an improved protocol for direct and indirect somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 is reported. Two types of explant used in the experiment were taken from rubber plantation at Felda Kemendor, Jasin, Melaka. Induction of direct somatic embryogenes...

Full description

Saved in:

Bibliographic Details
Main Author:	Bahrom, Siti Hidayatul Umairah
Format:	Thesis
Language:	English
Published:	2019
Online Access:	https://ir.uitm.edu.my/id/eprint/89115/1/89115.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.89115
record_format	uketd_dc
spelling	my-uitm-ir.891152024-04-15T08:38:58Z Screening method for initiation and maturation of somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 / Siti Hidayatul Umairah Bahrom 2019 Bahrom, Siti Hidayatul Umairah The establishment of an improved protocol for direct and indirect somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 is reported. Two types of explant used in the experiment were taken from rubber plantation at Felda Kemendor, Jasin, Melaka. Induction of direct somatic embryogenesis was achieved by using red young leaf explants cultured on MS media supplemented with 0.8 mg/L 2,4-D and 0.5 mg/L KN. Maturation of embryo from direct somatic embryogenesis was achieved by cultured the globular and early heart-shape embryo into MS media supplemented with 0.3 mg/L GA3, 0.3 mg/L BA, 0.1 mg/L IAA and 500 mg/L casein hydrolysates. For indirect somatic embryogenesis, the callus was induced by using red young petiole and red young leaf explants cultured on MS media supplemented with (1.0 mg/L – 2.0 mg/L) 2,4-D together with (0.5 and 1.0 mg/L) KN in 24 hours of darkness. The induction of somatic embryos from callus was achieved when red young petiole callus cultured on MS media supplemented with 0.3 mg/L GA3, 0.2 mg/L BA and 500 mg/L casein hydrolysates. The cultured were maintained in 24 hours of darkness. Maturation of somatic embryos was achieved when the embryogenic callus cultured on MS media supplemented with 0.3 mg/L GA3, 0.2 mg/L BA, 0.1 mg/L IAA and 500 mg/L casein hydrolysates in 16 hours of light and 8 hours of darkness. Future work in germination, regeneration and mass production of somatic embryos from young red leaf and young red petiole explants of H. brasiliensis Muell. Arg. var. RRIM 600 will be suitable for this species. 2019 Thesis https://ir.uitm.edu.my/id/eprint/89115/ https://ir.uitm.edu.my/id/eprint/89115/1/89115.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Plantation and Agrotechnology Awal, Asmah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Awal, Asmah
description	The establishment of an improved protocol for direct and indirect somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 is reported. Two types of explant used in the experiment were taken from rubber plantation at Felda Kemendor, Jasin, Melaka. Induction of direct somatic embryogenesis was achieved by using red young leaf explants cultured on MS media supplemented with 0.8 mg/L 2,4-D and 0.5 mg/L KN. Maturation of embryo from direct somatic embryogenesis was achieved by cultured the globular and early heart-shape embryo into MS media supplemented with 0.3 mg/L GA3, 0.3 mg/L BA, 0.1 mg/L IAA and 500 mg/L casein hydrolysates. For indirect somatic embryogenesis, the callus was induced by using red young petiole and red young leaf explants cultured on MS media supplemented with (1.0 mg/L – 2.0 mg/L) 2,4-D together with (0.5 and 1.0 mg/L) KN in 24 hours of darkness. The induction of somatic embryos from callus was achieved when red young petiole callus cultured on MS media supplemented with 0.3 mg/L GA3, 0.2 mg/L BA and 500 mg/L casein hydrolysates. The cultured were maintained in 24 hours of darkness. Maturation of somatic embryos was achieved when the embryogenic callus cultured on MS media supplemented with 0.3 mg/L GA3, 0.2 mg/L BA, 0.1 mg/L IAA and 500 mg/L casein hydrolysates in 16 hours of light and 8 hours of darkness. Future work in germination, regeneration and mass production of somatic embryos from young red leaf and young red petiole explants of H. brasiliensis Muell. Arg. var. RRIM 600 will be suitable for this species.
format	Thesis
qualification_level	Master's degree
author	Bahrom, Siti Hidayatul Umairah
spellingShingle	Bahrom, Siti Hidayatul Umairah Screening method for initiation and maturation of somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 / Siti Hidayatul Umairah Bahrom
author_facet	Bahrom, Siti Hidayatul Umairah
author_sort	Bahrom, Siti Hidayatul Umairah
title	Screening method for initiation and maturation of somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 / Siti Hidayatul Umairah Bahrom
title_short	Screening method for initiation and maturation of somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 / Siti Hidayatul Umairah Bahrom
title_full	Screening method for initiation and maturation of somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 / Siti Hidayatul Umairah Bahrom
title_fullStr	Screening method for initiation and maturation of somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 / Siti Hidayatul Umairah Bahrom
title_full_unstemmed	Screening method for initiation and maturation of somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 / Siti Hidayatul Umairah Bahrom
title_sort	screening method for initiation and maturation of somatic embryogenesis of hevea brasiliensis muell. arg. var. rrim 600 / siti hidayatul umairah bahrom
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Plantation and Agrotechnology
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/89115/1/89115.pdf
_version_	1804889800289812480

Screening method for initiation and maturation of somatic embryogenesis of Hevea brasiliensis Muell. Arg. var. RRIM 600 / Siti Hidayatul Umairah Bahrom

Similar Items