Determination of antioxidant activities, total phenolic content, total flavonoid content and ascorbic acid in pomegranate (punica granatum) and passion (passiflora edulis) fruit peel, seed and juice / Fara Syazana Ahmad Murshid

The aim of this study is to determine the total phenolic content, total flavonoid content, ascorbic acid content and their antioxidant activities by DPPH and FRAP assays on six samples which are the pomegranate fruit peel, seed, juice, passion fruit peel, seed, and juice. The samples are all extrac...

Full description

Saved in:

Bibliographic Details
Main Author:	Ahmad Murshid, Fara Syazana
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Plant anatomy
Online Access:	https://ir.uitm.edu.my/id/eprint/8922/1/FARA%20SYAZANA%20BT%20AHMAD%20MURSHID%2013_24.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.8922
record_format	uketd_dc
spelling	my-uitm-ir.89222022-07-05T06:16:06Z Determination of antioxidant activities, total phenolic content, total flavonoid content and ascorbic acid in pomegranate (punica granatum) and passion (passiflora edulis) fruit peel, seed and juice / Fara Syazana Ahmad Murshid 2013 Ahmad Murshid, Fara Syazana Plant anatomy The aim of this study is to determine the total phenolic content, total flavonoid content, ascorbic acid content and their antioxidant activities by DPPH and FRAP assays on six samples which are the pomegranate fruit peel, seed, juice, passion fruit peel, seed, and juice. The samples are all extracted by using ethanol to obtain the extracts before the determination of antioxidant content. For total phenolic content, it was determined using Folin-Cioclateu’s reagent with gallic acid as standard and measured at 750 nm. The highest amount of total phenolic content was in pomegranate peel with 182.71 ± 0.57 mg GAE/100 g sample and the lowest was in passion juice with 16.05 ± 0.00 mg GAE/100 g sample. The total flavonoid content was determined at 415 nm with rutin as standard and expressed as mg RE/100 g sample. The highest flavonoid content was in passion peel with 100.94 ± 0.00 mg RE/100 g sample and the lowest was in pomegranate seed with 5.00 ± 0/00 mg RE/100 g sample. Ascorbic acid content was determined according to AOAC method. The highest amount of ascorbic acid was in pomegranate peel with 62.84 ± 10.88 mg/100 g sample and the lowest was in passion seed with 15.71 ± 5.44 mg/100 g. Each sample was treated with DPPH and FRAP assay for the determination of antioxidant activities in each components and all the samples exhibited good antioxidant activities indicates the presence of antioxidant in the samples. All the samples values have significant difference at (p<0.05). Among the three components in pomegranate fruit, the peels shows the highest antioxidant capacity and activity, while for passion fruit, the peel also shows the highest antioxidant capacity and activities compared to its seed and juice 2013 Thesis https://ir.uitm.edu.my/id/eprint/8922/ https://ir.uitm.edu.my/id/eprint/8922/1/FARA%20SYAZANA%20BT%20AHMAD%20MURSHID%2013_24.pdf text en public degree Universiti Teknologi MARA Perpustakaan Tun Abdul Razak
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Plant anatomy
spellingShingle	Plant anatomy Ahmad Murshid, Fara Syazana Determination of antioxidant activities, total phenolic content, total flavonoid content and ascorbic acid in pomegranate (punica granatum) and passion (passiflora edulis) fruit peel, seed and juice / Fara Syazana Ahmad Murshid
description	The aim of this study is to determine the total phenolic content, total flavonoid content, ascorbic acid content and their antioxidant activities by DPPH and FRAP assays on six samples which are the pomegranate fruit peel, seed, juice, passion fruit peel, seed, and juice. The samples are all extracted by using ethanol to obtain the extracts before the determination of antioxidant content. For total phenolic content, it was determined using Folin-Cioclateu’s reagent with gallic acid as standard and measured at 750 nm. The highest amount of total phenolic content was in pomegranate peel with 182.71 ± 0.57 mg GAE/100 g sample and the lowest was in passion juice with 16.05 ± 0.00 mg GAE/100 g sample. The total flavonoid content was determined at 415 nm with rutin as standard and expressed as mg RE/100 g sample. The highest flavonoid content was in passion peel with 100.94 ± 0.00 mg RE/100 g sample and the lowest was in pomegranate seed with 5.00 ± 0/00 mg RE/100 g sample. Ascorbic acid content was determined according to AOAC method. The highest amount of ascorbic acid was in pomegranate peel with 62.84 ± 10.88 mg/100 g sample and the lowest was in passion seed with 15.71 ± 5.44 mg/100 g. Each sample was treated with DPPH and FRAP assay for the determination of antioxidant activities in each components and all the samples exhibited good antioxidant activities indicates the presence of antioxidant in the samples. All the samples values have significant difference at (p<0.05). Among the three components in pomegranate fruit, the peels shows the highest antioxidant capacity and activity, while for passion fruit, the peel also shows the highest antioxidant capacity and activities compared to its seed and juice
format	Thesis
qualification_level	Bachelor degree
author	Ahmad Murshid, Fara Syazana
author_facet	Ahmad Murshid, Fara Syazana
author_sort	Ahmad Murshid, Fara Syazana
title	Determination of antioxidant activities, total phenolic content, total flavonoid content and ascorbic acid in pomegranate (punica granatum) and passion (passiflora edulis) fruit peel, seed and juice / Fara Syazana Ahmad Murshid
title_short	Determination of antioxidant activities, total phenolic content, total flavonoid content and ascorbic acid in pomegranate (punica granatum) and passion (passiflora edulis) fruit peel, seed and juice / Fara Syazana Ahmad Murshid
title_full	Determination of antioxidant activities, total phenolic content, total flavonoid content and ascorbic acid in pomegranate (punica granatum) and passion (passiflora edulis) fruit peel, seed and juice / Fara Syazana Ahmad Murshid
title_fullStr	Determination of antioxidant activities, total phenolic content, total flavonoid content and ascorbic acid in pomegranate (punica granatum) and passion (passiflora edulis) fruit peel, seed and juice / Fara Syazana Ahmad Murshid
title_full_unstemmed	Determination of antioxidant activities, total phenolic content, total flavonoid content and ascorbic acid in pomegranate (punica granatum) and passion (passiflora edulis) fruit peel, seed and juice / Fara Syazana Ahmad Murshid
title_sort	determination of antioxidant activities, total phenolic content, total flavonoid content and ascorbic acid in pomegranate (punica granatum) and passion (passiflora edulis) fruit peel, seed and juice / fara syazana ahmad murshid
granting_institution	Universiti Teknologi MARA
granting_department	Perpustakaan Tun Abdul Razak
publishDate	2013
url	https://ir.uitm.edu.my/id/eprint/8922/1/FARA%20SYAZANA%20BT%20AHMAD%20MURSHID%2013_24.pdf
_version_	1783733147290042368

Determination of antioxidant activities, total phenolic content, total flavonoid content and ascorbic acid in pomegranate (punica granatum) and passion (passiflora edulis) fruit peel, seed and juice / Fara Syazana Ahmad Murshid

Similar Items