Identification and distribution of acetylcholinesterase inhibitory alkaloids from different locations in peninsular Malaysia / Nurul Ain Mohd Nor

A series of Lycopodium alkaloids, namely 6β-hydroxyhuperzine A (96), 16-hydroxyl huperzine A (318) and huperzine A (93), have been isolated from Huperzia phlegmaria. Their structure and relative stereo chemistry were elucidated on the basis of their spectral data and chemical correlations. A simple...

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Main Author:	Mohd Nor, Nurul Ain
Format:	Thesis
Language:	English
Published:	2018
Online Access:	https://ir.uitm.edu.my/id/eprint/89906/1/89906.pdf
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id	my-uitm-ir.89906
record_format	uketd_dc
spelling	my-uitm-ir.899062024-04-22T09:09:11Z Identification and distribution of acetylcholinesterase inhibitory alkaloids from different locations in peninsular Malaysia / Nurul Ain Mohd Nor 2018 Mohd Nor, Nurul Ain A series of Lycopodium alkaloids, namely 6β-hydroxyhuperzine A (96), 16-hydroxyl huperzine A (318) and huperzine A (93), have been isolated from Huperzia phlegmaria. Their structure and relative stereo chemistry were elucidated on the basis of their spectral data and chemical correlations. A simple and reliable high performance liquid chromatography (HPLC) method has been established for the analysis of the isolated compounds. The recovery rates were between 91 – 111 %. The within- and between-day precisions, expressed as R.S.D ranged from 0.5 % to 2.6 %. Good linear regression was observed, r2=0.999. The results demonstrate that this method is simple and suitable for quality control of this traditional Chinese medicinal herb. Additionally, the acetylcholinesterase inhibitory activity of isolated compounds was evaluated. The inhibition of cholinesterase activity was determined based on Ellman’s colorometric method. Colorimetric measurements were measured with a microplate reader at a wavelength of 405 nm. Galanthamine was used as a positive control. In conclusion, 6β-hydroxyhuperzine A (96) exhibited weak acetylcholinesterase inhibitory activity, while 16-hydroxyl huperzine A (318) did not show inhibitory effect (>200 μM) compared to huperzine A (93). 2018 Thesis https://ir.uitm.edu.my/id/eprint/89906/ https://ir.uitm.edu.my/id/eprint/89906/1/89906.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Choo, Chee Yan
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Choo, Chee Yan
description	A series of Lycopodium alkaloids, namely 6β-hydroxyhuperzine A (96), 16-hydroxyl huperzine A (318) and huperzine A (93), have been isolated from Huperzia phlegmaria. Their structure and relative stereo chemistry were elucidated on the basis of their spectral data and chemical correlations. A simple and reliable high performance liquid chromatography (HPLC) method has been established for the analysis of the isolated compounds. The recovery rates were between 91 – 111 %. The within- and between-day precisions, expressed as R.S.D ranged from 0.5 % to 2.6 %. Good linear regression was observed, r2=0.999. The results demonstrate that this method is simple and suitable for quality control of this traditional Chinese medicinal herb. Additionally, the acetylcholinesterase inhibitory activity of isolated compounds was evaluated. The inhibition of cholinesterase activity was determined based on Ellman’s colorometric method. Colorimetric measurements were measured with a microplate reader at a wavelength of 405 nm. Galanthamine was used as a positive control. In conclusion, 6β-hydroxyhuperzine A (96) exhibited weak acetylcholinesterase inhibitory activity, while 16-hydroxyl huperzine A (318) did not show inhibitory effect (>200 μM) compared to huperzine A (93).
format	Thesis
qualification_level	Master's degree
author	Mohd Nor, Nurul Ain
spellingShingle	Mohd Nor, Nurul Ain Identification and distribution of acetylcholinesterase inhibitory alkaloids from different locations in peninsular Malaysia / Nurul Ain Mohd Nor
author_facet	Mohd Nor, Nurul Ain
author_sort	Mohd Nor, Nurul Ain
title	Identification and distribution of acetylcholinesterase inhibitory alkaloids from different locations in peninsular Malaysia / Nurul Ain Mohd Nor
title_short	Identification and distribution of acetylcholinesterase inhibitory alkaloids from different locations in peninsular Malaysia / Nurul Ain Mohd Nor
title_full	Identification and distribution of acetylcholinesterase inhibitory alkaloids from different locations in peninsular Malaysia / Nurul Ain Mohd Nor
title_fullStr	Identification and distribution of acetylcholinesterase inhibitory alkaloids from different locations in peninsular Malaysia / Nurul Ain Mohd Nor
title_full_unstemmed	Identification and distribution of acetylcholinesterase inhibitory alkaloids from different locations in peninsular Malaysia / Nurul Ain Mohd Nor
title_sort	identification and distribution of acetylcholinesterase inhibitory alkaloids from different locations in peninsular malaysia / nurul ain mohd nor
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Pharmacy
publishDate	2018
url	https://ir.uitm.edu.my/id/eprint/89906/1/89906.pdf
_version_	1804889819643379712

Identification and distribution of acetylcholinesterase inhibitory alkaloids from different locations in peninsular Malaysia / Nurul Ain Mohd Nor

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