Studies on isolated crude bioactive compound(s) from ficus deltoidea for smooth muscle contractility potential / Muhammad Nasrul Hadi Azhar

Studies on isolated crude bioactive compound(s) from ficus deltoidea for smooth muscle contractility potential / Muhammad Nasrul Hadi Azhar

The isolation of the bioactive compound(s) from Ficus deltoidea was done by performing two different methods; traditional extraction technique using boiling step and conventional chemistry method. The former produced the crude water extract which will then partitioned with ethyl acetate producing wa...

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Main Author: Azhar, Muhammad Nasrul Hadi
Format: Thesis
Language:English
Published: 2019
Online Access:https://ir.uitm.edu.my/id/eprint/93116/1/93116.pdf
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spelling my-uitm-ir.931162024-09-09T07:25:54Z Studies on isolated crude bioactive compound(s) from ficus deltoidea for smooth muscle contractility potential / Muhammad Nasrul Hadi Azhar 2019 Azhar, Muhammad Nasrul Hadi The isolation of the bioactive compound(s) from Ficus deltoidea was done by performing two different methods; traditional extraction technique using boiling step and conventional chemistry method. The former produced the crude water extract which will then partitioned with ethyl acetate producing water and ethyl acetate fractions. The latter involved sequential partioning of the crude methanol extract with solvent of different polarity (hexane, chloroform, ethyl acetate and distilled water). Angiotensin converting enzyme (ACE) and acetylcholinesterase (AChE) enzyme assays were used as bio-guided assays to search for the bioactive compound(s). Samples that have good activity were further separated and purified using size exclusion-, silica gel- and Celite® gel- column chromatography. Samples with good activity were also subjected to protein identification,using SDS - PAGE and LC/QToF MS approaches. From the traditional extraction method, water fraction (WF) and water fraction subfraction 2 (WFSF2) showed good inhibitory activity towards ACE (60.360±0.508% and 72.646±15.012% respectively). Both samples have very minimal inhibitory activity towards AChE (5.535±0.849% and 13.835±3.249% respectively). Water fractions showed the presence of rubber elongation factor protein, cdc2 kinase and non-specific serine/threonine protein kinase. Meanwhile, water fraction subfraction 2 only showed the presence of actin. The extract and fractions from the conventional solvent based method did not show good response towards the assays. Hexane soluble extract fraction 5 subfraction 1 (HSEF5SF1) and chloroform soluble extract fraction 1 subfraction l(CSEFlSFl) were sent for GCMS analysis. 2019 Thesis https://ir.uitm.edu.my/id/eprint/93116/ https://ir.uitm.edu.my/id/eprint/93116/1/93116.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Yasin, Yamin Aminudin, Norhaniza
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Yasin, Yamin
Aminudin, Norhaniza
description The isolation of the bioactive compound(s) from Ficus deltoidea was done by performing two different methods; traditional extraction technique using boiling step and conventional chemistry method. The former produced the crude water extract which will then partitioned with ethyl acetate producing water and ethyl acetate fractions. The latter involved sequential partioning of the crude methanol extract with solvent of different polarity (hexane, chloroform, ethyl acetate and distilled water). Angiotensin converting enzyme (ACE) and acetylcholinesterase (AChE) enzyme assays were used as bio-guided assays to search for the bioactive compound(s). Samples that have good activity were further separated and purified using size exclusion-, silica gel- and Celite® gel- column chromatography. Samples with good activity were also subjected to protein identification,using SDS - PAGE and LC/QToF MS approaches. From the traditional extraction method, water fraction (WF) and water fraction subfraction 2 (WFSF2) showed good inhibitory activity towards ACE (60.360±0.508% and 72.646±15.012% respectively). Both samples have very minimal inhibitory activity towards AChE (5.535±0.849% and 13.835±3.249% respectively). Water fractions showed the presence of rubber elongation factor protein, cdc2 kinase and non-specific serine/threonine protein kinase. Meanwhile, water fraction subfraction 2 only showed the presence of actin. The extract and fractions from the conventional solvent based method did not show good response towards the assays. Hexane soluble extract fraction 5 subfraction 1 (HSEF5SF1) and chloroform soluble extract fraction 1 subfraction l(CSEFlSFl) were sent for GCMS analysis.
format Thesis
qualification_level Master's degree
author Azhar, Muhammad Nasrul Hadi
spellingShingle Azhar, Muhammad Nasrul Hadi
Studies on isolated crude bioactive compound(s) from ficus deltoidea for smooth muscle contractility potential / Muhammad Nasrul Hadi Azhar
author_facet Azhar, Muhammad Nasrul Hadi
author_sort Azhar, Muhammad Nasrul Hadi
title Studies on isolated crude bioactive compound(s) from ficus deltoidea for smooth muscle contractility potential / Muhammad Nasrul Hadi Azhar
title_short Studies on isolated crude bioactive compound(s) from ficus deltoidea for smooth muscle contractility potential / Muhammad Nasrul Hadi Azhar
title_full Studies on isolated crude bioactive compound(s) from ficus deltoidea for smooth muscle contractility potential / Muhammad Nasrul Hadi Azhar
title_fullStr Studies on isolated crude bioactive compound(s) from ficus deltoidea for smooth muscle contractility potential / Muhammad Nasrul Hadi Azhar
title_full_unstemmed Studies on isolated crude bioactive compound(s) from ficus deltoidea for smooth muscle contractility potential / Muhammad Nasrul Hadi Azhar
title_sort studies on isolated crude bioactive compound(s) from ficus deltoidea for smooth muscle contractility potential / muhammad nasrul hadi azhar
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Applied Sciences
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/93116/1/93116.pdf
_version_ 1811768766539759616

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