The optimal tissue thickness for histological diagnosis of tuberculosis / Aida Nurbaiti Arbain

Introduction. Tuberculosis is an important infectious disease caused by the bacillus Mycobacterium tuberculosis. It can cause pulmonary and extrapulmonary tuberculosis. There are several acid-fast bacilli (AFB) stains available for the formalin fixed paraffin embedded specimens. These include Kinyou...

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Main Author:	Arbain, Aida Nurbaiti
Format:	Thesis
Language:	English
Published:	2022
Subjects:	Tuberculosis
Online Access:	https://ir.uitm.edu.my/id/eprint/95202/1/95202.PDF
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id	my-uitm-ir.95202
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spelling	my-uitm-ir.952022024-08-21T04:10:51Z The optimal tissue thickness for histological diagnosis of tuberculosis / Aida Nurbaiti Arbain 2022 Arbain, Aida Nurbaiti Tuberculosis Introduction. Tuberculosis is an important infectious disease caused by the bacillus Mycobacterium tuberculosis. It can cause pulmonary and extrapulmonary tuberculosis. There are several acid-fast bacilli (AFB) stains available for the formalin fixed paraffin embedded specimens. These include Kinyoun stain, a faster and cheaper option for diagnosing tuberculosis on tissue specimens. There is no available published study on the optimal tissue thickness for AFB staining; hence this study was conducted to find the best thickness in facilitating AFB detection. Materials and methods. This is a retrospective study. A total of 84 granuloma cases were included, of which 29 cases were positive for Mycobacterium Tuberculosis culture and another 55 cases were negative. All cases were subjected to Kinyoun stain at 3 microns, 5 microns and 7 microns thick sections for AFB detection. The AFB detection was done by the researcher and followed by I 0% random screening by two pathologists. The sensitivity, specificity, positive predictive value and negative predictive value of each thickness were calculated and compared. Results. In this study, 7 microns thickness shows the highest sensitivity (48.26%) compared to the 3 microns (31.03%) and 5 microns thickness (37.93%). 7 microns thickness also shows higher specificity than 5 microns thickness (92.73%) and this result is similar to 3 microns thickness. Conclusion. The 7 microns thick section yielded the highest sensitivity in detecting AFB using Kinyoun stain. Faculty of Medicine 2022 Thesis https://ir.uitm.edu.my/id/eprint/95202/ https://ir.uitm.edu.my/id/eprint/95202/1/95202.PDF text en public masters Universiti Teknologi MARA Faculty of Medicine
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Tuberculosis
spellingShingle	Tuberculosis Arbain, Aida Nurbaiti The optimal tissue thickness for histological diagnosis of tuberculosis / Aida Nurbaiti Arbain
description	Introduction. Tuberculosis is an important infectious disease caused by the bacillus Mycobacterium tuberculosis. It can cause pulmonary and extrapulmonary tuberculosis. There are several acid-fast bacilli (AFB) stains available for the formalin fixed paraffin embedded specimens. These include Kinyoun stain, a faster and cheaper option for diagnosing tuberculosis on tissue specimens. There is no available published study on the optimal tissue thickness for AFB staining; hence this study was conducted to find the best thickness in facilitating AFB detection. Materials and methods. This is a retrospective study. A total of 84 granuloma cases were included, of which 29 cases were positive for Mycobacterium Tuberculosis culture and another 55 cases were negative. All cases were subjected to Kinyoun stain at 3 microns, 5 microns and 7 microns thick sections for AFB detection. The AFB detection was done by the researcher and followed by I 0% random screening by two pathologists. The sensitivity, specificity, positive predictive value and negative predictive value of each thickness were calculated and compared. Results. In this study, 7 microns thickness shows the highest sensitivity (48.26%) compared to the 3 microns (31.03%) and 5 microns thickness (37.93%). 7 microns thickness also shows higher specificity than 5 microns thickness (92.73%) and this result is similar to 3 microns thickness. Conclusion. The 7 microns thick section yielded the highest sensitivity in detecting AFB using Kinyoun stain.
format	Thesis
qualification_level	Master's degree
author	Arbain, Aida Nurbaiti
author_facet	Arbain, Aida Nurbaiti
author_sort	Arbain, Aida Nurbaiti
title	The optimal tissue thickness for histological diagnosis of tuberculosis / Aida Nurbaiti Arbain
title_short	The optimal tissue thickness for histological diagnosis of tuberculosis / Aida Nurbaiti Arbain
title_full	The optimal tissue thickness for histological diagnosis of tuberculosis / Aida Nurbaiti Arbain
title_fullStr	The optimal tissue thickness for histological diagnosis of tuberculosis / Aida Nurbaiti Arbain
title_full_unstemmed	The optimal tissue thickness for histological diagnosis of tuberculosis / Aida Nurbaiti Arbain
title_sort	optimal tissue thickness for histological diagnosis of tuberculosis / aida nurbaiti arbain
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Medicine
publishDate	2022
url	https://ir.uitm.edu.my/id/eprint/95202/1/95202.PDF
_version_	1811768812117164032

The optimal tissue thickness for histological diagnosis of tuberculosis / Aida Nurbaiti Arbain

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