Association between gluten sensitivity and epilepsy based on HLA-DQ genotyping together with celiac disease related antibody / Nur Malina Mat Reffien

Introduction : Gluten sensitivity (GS) is a common autoimmune disease that has been reported to affect 1 in every 100 people worldwide. (I, 2) GS is associated with several neurological conditions including epilepsy. (3) About 95% of people diagnosed with gluten intolerance were genetically predispo...

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Main Author:	Mat Reffien, Nur Malina
Format:	Thesis
Language:	English
Published:	2023
Subjects:	Diseases of the digestive system Gastroenterology
Online Access:	https://ir.uitm.edu.my/id/eprint/95290/1/95290.PDF
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spelling	my-uitm-ir.952902024-08-21T02:50:15Z Association between gluten sensitivity and epilepsy based on HLA-DQ genotyping together with celiac disease related antibody / Nur Malina Mat Reffien 2023 Mat Reffien, Nur Malina Diseases of the digestive system. Gastroenterology Introduction : Gluten sensitivity (GS) is a common autoimmune disease that has been reported to affect 1 in every 100 people worldwide. (I, 2) GS is associated with several neurological conditions including epilepsy. (3) About 95% of people diagnosed with gluten intolerance were genetically predisposed through the human leukocyte antigen (HLA), HLA-DQ 2 and HLA-DQ 8. (4, 5) e purpose of this study is to investigate the association between epilepsy and gluten sensitivity based on HLA-DQ genotyping, alpha-gliadin antibody (AGA), and tissue transglutaminase antibody (anti-tTG). Method: A case-control study was conducted with a target sample size of 100 patients. 50 epilepsy patients and 50 non-epileptic adults matched for age, gender, and race were recruited. All patients were subjected to an interview, clinical examination and venous blood sampling. AGA and anti-tTG antibodies were detected using a commercially available ELISA kit. The blood sample were also sent for genotRefyping of HLA-DQ alleles represented by 6 single nucleotide polymorphisms (SNPs) [(i) DQ2.2 (rs2395182; rs7775228 rs4713586); (ii) DQ2.5 rs2187668; (iii) DQ7 (rs4639334) and (iv) DQ 8 (rs7454108)]. Results: The overall seroprevalence of gluten sensitivity in the study group was 40%. Out of this 21 (42%) patients were from epilepsy group. HLA-DQ 2.2 genotype was found to be strongly associated with epilepsy where 9 epileptic patients were positive for HLA-DQ 2.2 [p-value 0.003]. However, the seroprevalence of HLA-DQ 8 was 18 (36%) in the epileptic group and 19 (38%) in the control group [p-value=0.836]. The AGA was positive in 4 (8%) epilepsy patients while anti-tTG antibody was negative in both study groups. Overall, there was no association seen between gluten sensitivity seropositivity and epilepsy in our study [OR 0.85 (CI 0.380-1.885), (p-value 0.838)]. Nevertheless, we found that 8 out of 21 epileptic patients with gluten sensitivity used more than 1 anti-seizure medication. (p-value 0.014). Conclusion: Our study showed no association between GS and epilepsy. However, the seroprevalence of HLA-DQ 2.2 genotype was significantly higher in epileptic patients and those epileptic patients with GS required multiple anti-seizure medications. Faculty of Medicine 2023 Thesis https://ir.uitm.edu.my/id/eprint/95290/ https://ir.uitm.edu.my/id/eprint/95290/1/95290.PDF text en public masters Universiti Teknologi MARA Faculty of Medicine
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Diseases of the digestive system Gastroenterology
spellingShingle	Diseases of the digestive system Gastroenterology Mat Reffien, Nur Malina Association between gluten sensitivity and epilepsy based on HLA-DQ genotyping together with celiac disease related antibody / Nur Malina Mat Reffien
description	Introduction : Gluten sensitivity (GS) is a common autoimmune disease that has been reported to affect 1 in every 100 people worldwide. (I, 2) GS is associated with several neurological conditions including epilepsy. (3) About 95% of people diagnosed with gluten intolerance were genetically predisposed through the human leukocyte antigen (HLA), HLA-DQ 2 and HLA-DQ 8. (4, 5) e purpose of this study is to investigate the association between epilepsy and gluten sensitivity based on HLA-DQ genotyping, alpha-gliadin antibody (AGA), and tissue transglutaminase antibody (anti-tTG). Method: A case-control study was conducted with a target sample size of 100 patients. 50 epilepsy patients and 50 non-epileptic adults matched for age, gender, and race were recruited. All patients were subjected to an interview, clinical examination and venous blood sampling. AGA and anti-tTG antibodies were detected using a commercially available ELISA kit. The blood sample were also sent for genotRefyping of HLA-DQ alleles represented by 6 single nucleotide polymorphisms (SNPs) [(i) DQ2.2 (rs2395182; rs7775228 rs4713586); (ii) DQ2.5 rs2187668; (iii) DQ7 (rs4639334) and (iv) DQ 8 (rs7454108)]. Results: The overall seroprevalence of gluten sensitivity in the study group was 40%. Out of this 21 (42%) patients were from epilepsy group. HLA-DQ 2.2 genotype was found to be strongly associated with epilepsy where 9 epileptic patients were positive for HLA-DQ 2.2 [p-value 0.003]. However, the seroprevalence of HLA-DQ 8 was 18 (36%) in the epileptic group and 19 (38%) in the control group [p-value=0.836]. The AGA was positive in 4 (8%) epilepsy patients while anti-tTG antibody was negative in both study groups. Overall, there was no association seen between gluten sensitivity seropositivity and epilepsy in our study [OR 0.85 (CI 0.380-1.885), (p-value 0.838)]. Nevertheless, we found that 8 out of 21 epileptic patients with gluten sensitivity used more than 1 anti-seizure medication. (p-value 0.014). Conclusion: Our study showed no association between GS and epilepsy. However, the seroprevalence of HLA-DQ 2.2 genotype was significantly higher in epileptic patients and those epileptic patients with GS required multiple anti-seizure medications.
format	Thesis
qualification_level	Master's degree
author	Mat Reffien, Nur Malina
author_facet	Mat Reffien, Nur Malina
author_sort	Mat Reffien, Nur Malina
title	Association between gluten sensitivity and epilepsy based on HLA-DQ genotyping together with celiac disease related antibody / Nur Malina Mat Reffien
title_short	Association between gluten sensitivity and epilepsy based on HLA-DQ genotyping together with celiac disease related antibody / Nur Malina Mat Reffien
title_full	Association between gluten sensitivity and epilepsy based on HLA-DQ genotyping together with celiac disease related antibody / Nur Malina Mat Reffien
title_fullStr	Association between gluten sensitivity and epilepsy based on HLA-DQ genotyping together with celiac disease related antibody / Nur Malina Mat Reffien
title_full_unstemmed	Association between gluten sensitivity and epilepsy based on HLA-DQ genotyping together with celiac disease related antibody / Nur Malina Mat Reffien
title_sort	association between gluten sensitivity and epilepsy based on hla-dq genotyping together with celiac disease related antibody / nur malina mat reffien
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Medicine
publishDate	2023
url	https://ir.uitm.edu.my/id/eprint/95290/1/95290.PDF
_version_	1811768815639330816

Association between gluten sensitivity and epilepsy based on HLA-DQ genotyping together with celiac disease related antibody / Nur Malina Mat Reffien

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