Genotyping of CYP2C9 using DNA from hair roots, saliva, fingernails, buccal cell swabs and blood / Maznuraini Zainuddin

Background: Blood is an excellent source of genomic DNA. However, blood sampling is invasive, painful and requires complicated handling and transport. Therefore, we intend to obtain DNA from other sources such as hairs, saliva, fingernails and buccal cell as an alternative to blood. The samplings of...

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Main Author:	Zainuddin, Maznuraini
Format:	Thesis
Language:	English
Published:	2005
Subjects:	DNA Deoxyribonucleic acids Extraction (Chemistry)
Online Access:	https://ir.uitm.edu.my/id/eprint/98478/1/98478.PDF
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id	my-uitm-ir.98478
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spelling	my-uitm-ir.984782024-08-27T18:12:30Z Genotyping of CYP2C9 using DNA from hair roots, saliva, fingernails, buccal cell swabs and blood / Maznuraini Zainuddin 2005 Zainuddin, Maznuraini DNA. Deoxyribonucleic acids Extraction (Chemistry) Background: Blood is an excellent source of genomic DNA. However, blood sampling is invasive, painful and requires complicated handling and transport. Therefore, we intend to obtain DNA from other sources such as hairs, saliva, fingernails and buccal cell as an alternative to blood. The samplings of these non-blood specimens are painless, non-invasive, convenient and accessible as compared to blood as a source of DNA for genotyping test. Aim: The aim of the study was to develop a simple DNA extraction method from sources other than blood. These DNA could thus be used for genotyping test of the polymorphic CYP enzymes whereby CYP2C93 was used as prototype. Method: Hairs, saliva, fingernails, buccal cell swabs and blood were taken from 5 healthy unrelated volunteers. They are students of Faculty of Pharmacy, Universiti Teknologi MARA. The samples were subjected to DNA extraction. DNA were extracted from hair roots, saliva, fingernails, buccal cell swabs by using 2 different extraction methods which were one step DNA extraction method with and without Chelex. The blood samples were extracted by using Quick Gene DNA whole blood kit S (DB-S). The extracted DNA was used for subsequent PCR based genotyping of CYP2C93. Results: DNA was successfully extracted from hair roots, saliva, fingernails and buccal cell swabs by using both extraction methods. However, the amount of DNA extracted was not as high yield as compared to DNA extracted from blood. The DNA obtained from these different sources were able to be amplified by PCR and thus useful for genotyping of CYP2C93 in comparison to DNA extracted from blood. Conclusion: The DNA extracted from hair roots, saliva, fingernails and buccal cell swabs were useful sources of genomic DNA and thus could be used in genotyping of CYP2C93. 2005 Thesis https://ir.uitm.edu.my/id/eprint/98478/ https://ir.uitm.edu.my/id/eprint/98478/1/98478.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Kek, Teh Lay
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Kek, Teh Lay
topic	DNA Deoxyribonucleic acids Extraction (Chemistry)
spellingShingle	DNA Deoxyribonucleic acids Extraction (Chemistry) Zainuddin, Maznuraini Genotyping of CYP2C9 using DNA from hair roots, saliva, fingernails, buccal cell swabs and blood / Maznuraini Zainuddin
description	Background: Blood is an excellent source of genomic DNA. However, blood sampling is invasive, painful and requires complicated handling and transport. Therefore, we intend to obtain DNA from other sources such as hairs, saliva, fingernails and buccal cell as an alternative to blood. The samplings of these non-blood specimens are painless, non-invasive, convenient and accessible as compared to blood as a source of DNA for genotyping test. Aim: The aim of the study was to develop a simple DNA extraction method from sources other than blood. These DNA could thus be used for genotyping test of the polymorphic CYP enzymes whereby CYP2C93 was used as prototype. Method: Hairs, saliva, fingernails, buccal cell swabs and blood were taken from 5 healthy unrelated volunteers. They are students of Faculty of Pharmacy, Universiti Teknologi MARA. The samples were subjected to DNA extraction. DNA were extracted from hair roots, saliva, fingernails, buccal cell swabs by using 2 different extraction methods which were one step DNA extraction method with and without Chelex. The blood samples were extracted by using Quick Gene DNA whole blood kit S (DB-S). The extracted DNA was used for subsequent PCR based genotyping of CYP2C93. Results: DNA was successfully extracted from hair roots, saliva, fingernails and buccal cell swabs by using both extraction methods. However, the amount of DNA extracted was not as high yield as compared to DNA extracted from blood. The DNA obtained from these different sources were able to be amplified by PCR and thus useful for genotyping of CYP2C93 in comparison to DNA extracted from blood. Conclusion: The DNA extracted from hair roots, saliva, fingernails and buccal cell swabs were useful sources of genomic DNA and thus could be used in genotyping of CYP2C93.
format	Thesis
qualification_level	Bachelor degree
author	Zainuddin, Maznuraini
author_facet	Zainuddin, Maznuraini
author_sort	Zainuddin, Maznuraini
title	Genotyping of CYP2C9 using DNA from hair roots, saliva, fingernails, buccal cell swabs and blood / Maznuraini Zainuddin
title_short	Genotyping of CYP2C9 using DNA from hair roots, saliva, fingernails, buccal cell swabs and blood / Maznuraini Zainuddin
title_full	Genotyping of CYP2C9 using DNA from hair roots, saliva, fingernails, buccal cell swabs and blood / Maznuraini Zainuddin
title_fullStr	Genotyping of CYP2C9 using DNA from hair roots, saliva, fingernails, buccal cell swabs and blood / Maznuraini Zainuddin
title_full_unstemmed	Genotyping of CYP2C9 using DNA from hair roots, saliva, fingernails, buccal cell swabs and blood / Maznuraini Zainuddin
title_sort	genotyping of cyp2c9 using dna from hair roots, saliva, fingernails, buccal cell swabs and blood / maznuraini zainuddin
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2005
url	https://ir.uitm.edu.my/id/eprint/98478/1/98478.PDF
_version_	1811768924401827840

Genotyping of CYP2C9 using DNA from hair roots, saliva, fingernails, buccal cell swabs and blood / Maznuraini Zainuddin

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