Optimization of PCR method for the polymorphic detection of beta-2 adrenergic receptor gene / Afriza Aini Mohamad Afzal

The aim of this study is to perform a one step direct PCR method in identifying polymorphisms of five alleles of interest of the beta-2 adrenoceptor gene (Arg 16/ Gly 16, -20T/C, Gln27/ Glu27, -47T/C and Thr164/ Ile164). This one step direct PCR is also expected to produce better results compared to...

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Main Author:	Mohamad Afzal, Afriza Aini
Format:	Thesis
Language:	English
Published:	2006
Subjects:	RM Therapeutics Pharmacology RS Pharmacy and materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/99089/1/99089.PDF
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id	my-uitm-ir.99089
record_format	uketd_dc
spelling	my-uitm-ir.990892024-07-24T03:39:29Z Optimization of PCR method for the polymorphic detection of beta-2 adrenergic receptor gene / Afriza Aini Mohamad Afzal 2006 Mohamad Afzal, Afriza Aini RM Therapeutics. Pharmacology RS Pharmacy and materia medica The aim of this study is to perform a one step direct PCR method in identifying polymorphisms of five alleles of interest of the beta-2 adrenoceptor gene (Arg 16/ Gly 16, -20T/C, Gln27/ Glu27, -47T/C and Thr164/ Ile164). This one step direct PCR is also expected to produce better results compared to a previous study with the same alleles of interest but using a different method which was the two steps PCR. The method used in the study was multiple allele specific reaction which can allow simultaneous amplification of the five beta-2 adrenoceptor alleles. Specific primers were used to complement the target sequence and produce specific bands of interest. The PCR method was optimized for reproducibility and specificity. Few parameters were frequently adjusted to produce the desired results. The parameters include annealing temperature, cycling conditions, primers and Taq polymerase concentration. At the end of the study, the results were unsuccessful due to few problems and possible causes that can be improved in future studies. 2006 Thesis https://ir.uitm.edu.my/id/eprint/99089/ https://ir.uitm.edu.my/id/eprint/99089/1/99089.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Kek, Teh Lay
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Kek, Teh Lay
topic	RM Therapeutics Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics Pharmacology RS Pharmacy and materia medica Mohamad Afzal, Afriza Aini Optimization of PCR method for the polymorphic detection of beta-2 adrenergic receptor gene / Afriza Aini Mohamad Afzal
description	The aim of this study is to perform a one step direct PCR method in identifying polymorphisms of five alleles of interest of the beta-2 adrenoceptor gene (Arg 16/ Gly 16, -20T/C, Gln27/ Glu27, -47T/C and Thr164/ Ile164). This one step direct PCR is also expected to produce better results compared to a previous study with the same alleles of interest but using a different method which was the two steps PCR. The method used in the study was multiple allele specific reaction which can allow simultaneous amplification of the five beta-2 adrenoceptor alleles. Specific primers were used to complement the target sequence and produce specific bands of interest. The PCR method was optimized for reproducibility and specificity. Few parameters were frequently adjusted to produce the desired results. The parameters include annealing temperature, cycling conditions, primers and Taq polymerase concentration. At the end of the study, the results were unsuccessful due to few problems and possible causes that can be improved in future studies.
format	Thesis
qualification_level	Bachelor degree
author	Mohamad Afzal, Afriza Aini
author_facet	Mohamad Afzal, Afriza Aini
author_sort	Mohamad Afzal, Afriza Aini
title	Optimization of PCR method for the polymorphic detection of beta-2 adrenergic receptor gene / Afriza Aini Mohamad Afzal
title_short	Optimization of PCR method for the polymorphic detection of beta-2 adrenergic receptor gene / Afriza Aini Mohamad Afzal
title_full	Optimization of PCR method for the polymorphic detection of beta-2 adrenergic receptor gene / Afriza Aini Mohamad Afzal
title_fullStr	Optimization of PCR method for the polymorphic detection of beta-2 adrenergic receptor gene / Afriza Aini Mohamad Afzal
title_full_unstemmed	Optimization of PCR method for the polymorphic detection of beta-2 adrenergic receptor gene / Afriza Aini Mohamad Afzal
title_sort	optimization of pcr method for the polymorphic detection of beta-2 adrenergic receptor gene / afriza aini mohamad afzal
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2006
url	https://ir.uitm.edu.my/id/eprint/99089/1/99089.PDF
_version_	1811768981506228224

Optimization of PCR method for the polymorphic detection of beta-2 adrenergic receptor gene / Afriza Aini Mohamad Afzal

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