Construction of pBAD/TOPO® thio fusion-pla2 gene cassette in escherichia coli / Hanis Hanum Zulkifly

Aim - To construct pBAD/TOPO® Thio Fusion-p/a1 gene cassette in Escherichia coli Method - The method of this study started of with conformation of the original clone (p/a1) synthesized by First BASE Laboratories followed by the amplification of the gene (pla2) using PCR. Then, the amplified gene w...

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Main Author:	Zulkifly, Hanis Hanum
Format:	Thesis
Language:	English
Published:	2006
Subjects:	RM Therapeutics Pharmacology RS Pharmacy and materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/99130/1/99130.PDF
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id	my-uitm-ir.99130
record_format	uketd_dc
spelling	my-uitm-ir.991302024-07-24T03:39:09Z Construction of pBAD/TOPO® thio fusion-pla2 gene cassette in escherichia coli / Hanis Hanum Zulkifly 2006 Zulkifly, Hanis Hanum RM Therapeutics. Pharmacology RS Pharmacy and materia medica Aim - To construct pBAD/TOPO® Thio Fusion-p/a1 gene cassette in Escherichia coli Method - The method of this study started of with conformation of the original clone (p/a1) synthesized by First BASE Laboratories followed by the amplification of the gene (pla2) using PCR. Then, the amplified gene was ligated into a vector which is pBAD/TOPO® Thio Fusion. The entire plasmid was then transformed into a bacterial host, £. coli strain TOP I 0. Analysis of positive recombinant was done by restriction digestion. analyzing PCR and DNA sequencing. Prior to performing restriction digestion, PCR and DNA sequencing, the plasmid which carries the gene of interest (GOI) must be extracted from the bacterial host. Extraction was done using commercial DNA extraction kit (Wizard'" Plus Minipreps DNA Purification System, Promega). Conclusion - This study represent the construction of bacterial expression system for heterologous expression of pla, in £. coli. It can be concluded that it is a success to construct pBAD/TOPOl!, Thio Fusion-o/a, plasmid in £. coli. It has been proven that the nucleotide sequence of pla2 gene exhibits high homology to the corresponding region of the porcine PLA2 sequence. 2006 Thesis https://ir.uitm.edu.my/id/eprint/99130/ https://ir.uitm.edu.my/id/eprint/99130/1/99130.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Ahmad, Norazlina
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ahmad, Norazlina
topic	RM Therapeutics Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics Pharmacology RS Pharmacy and materia medica Zulkifly, Hanis Hanum Construction of pBAD/TOPO® thio fusion-pla2 gene cassette in escherichia coli / Hanis Hanum Zulkifly
description	Aim - To construct pBAD/TOPO® Thio Fusion-p/a1 gene cassette in Escherichia coli Method - The method of this study started of with conformation of the original clone (p/a1) synthesized by First BASE Laboratories followed by the amplification of the gene (pla2) using PCR. Then, the amplified gene was ligated into a vector which is pBAD/TOPO® Thio Fusion. The entire plasmid was then transformed into a bacterial host, £. coli strain TOP I 0. Analysis of positive recombinant was done by restriction digestion. analyzing PCR and DNA sequencing. Prior to performing restriction digestion, PCR and DNA sequencing, the plasmid which carries the gene of interest (GOI) must be extracted from the bacterial host. Extraction was done using commercial DNA extraction kit (Wizard'" Plus Minipreps DNA Purification System, Promega). Conclusion - This study represent the construction of bacterial expression system for heterologous expression of pla, in £. coli. It can be concluded that it is a success to construct pBAD/TOPOl!, Thio Fusion-o/a, plasmid in £. coli. It has been proven that the nucleotide sequence of pla2 gene exhibits high homology to the corresponding region of the porcine PLA2 sequence.
format	Thesis
qualification_level	Bachelor degree
author	Zulkifly, Hanis Hanum
author_facet	Zulkifly, Hanis Hanum
author_sort	Zulkifly, Hanis Hanum
title	Construction of pBAD/TOPO® thio fusion-pla2 gene cassette in escherichia coli / Hanis Hanum Zulkifly
title_short	Construction of pBAD/TOPO® thio fusion-pla2 gene cassette in escherichia coli / Hanis Hanum Zulkifly
title_full	Construction of pBAD/TOPO® thio fusion-pla2 gene cassette in escherichia coli / Hanis Hanum Zulkifly
title_fullStr	Construction of pBAD/TOPO® thio fusion-pla2 gene cassette in escherichia coli / Hanis Hanum Zulkifly
title_full_unstemmed	Construction of pBAD/TOPO® thio fusion-pla2 gene cassette in escherichia coli / Hanis Hanum Zulkifly
title_sort	construction of pbad/topo® thio fusion-pla2 gene cassette in escherichia coli / hanis hanum zulkifly
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2006
url	https://ir.uitm.edu.my/id/eprint/99130/1/99130.PDF
_version_	1811768990067851264

Construction of pBAD/TOPO® thio fusion-pla2 gene cassette in escherichia coli / Hanis Hanum Zulkifly

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