Development of brain glutamate microbiosensor using platinum (Pt) electrode and application of in vivo / Siti Kartika Hamdan

Monitoring of extracellular brain glutamate (Glu) level by microbiosensor is a promising approach for further investigation of the important role of neurotransmitter. The neurotransmitter glutamate has shown in development of brain function including cognition, memory and learning process. Glutamate...

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Main Author:	Hamdan, Siti Kartika
Format:	Thesis
Language:	English
Published:	2016
Subjects:	General
Online Access:	https://ir.uitm.edu.my/id/eprint/99136/1/99136.pdf
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id	my-uitm-ir.99136
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spelling	my-uitm-ir.991362024-12-12T02:13:00Z Development of brain glutamate microbiosensor using platinum (Pt) electrode and application of in vivo / Siti Kartika Hamdan 2016 Hamdan, Siti Kartika General Monitoring of extracellular brain glutamate (Glu) level by microbiosensor is a promising approach for further investigation of the important role of neurotransmitter. The neurotransmitter glutamate has shown in development of brain function including cognition, memory and learning process. Glutamate microbiosensor should serve high sensitivity which able to response for low concentration, high selectivity from interferences, fast and accurate result. Surface teflon encapsulated platinum (Pt) electrode disk design of internal size diameter 50 µm, electropolymerized with poly-o-phenylenediamine (PPD), layered with nafion (Naf), immobilized with glutamate oxidase (GluOx) and cross-linked glutaraldehyde (GA) (Pt50/PPD/Naf/GluOx/GA) has chosen in glutamate detection and performed promising glutamate biosensor using constant potential amperometry (CPA) where Michaelis-Menten for glutamate response, Jmax = 120 µAcm-2, KM= 60 µM and limit of detection (LOD) is 2.5 µM ± 0.5 µM were obtained. The response time was ~9s which is three times faster as compared to Pt50/PPD/GluOx/Ga. The developed glutamate microbiosensor have established an excellent in rejection against ascorbic acid (AA) and other interferences. This electrode designed configuration shows good sensitivity in neutral pH range at pH 7.5. The stability of the electrode was stable until 3 days and kept in cold temperature at 4oC. The reduced enzyme activity on the electrode was due to dissolution of the enzymatic membrane during storage and causing the sensitivity of electrode less stable along the period. In vivo studied shows that data sampling rate of electrode without enzyme (Pt50/PPD/Naf) and with enzyme (Pt50/PPD/Naf/GluOx/GA) were 0.0022 µA and 0.0039 µA, respectively. The ability of glutamate microbiosensor in monitoring the presence of glutamate in the prefrontal cortex of anaesthetized rats was proven. 2016 Thesis https://ir.uitm.edu.my/id/eprint/99136/ https://ir.uitm.edu.my/id/eprint/99136/1/99136.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Mohd Zain, Zainiharyati
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mohd Zain, Zainiharyati
topic	General
spellingShingle	General Hamdan, Siti Kartika Development of brain glutamate microbiosensor using platinum (Pt) electrode and application of in vivo / Siti Kartika Hamdan
description	Monitoring of extracellular brain glutamate (Glu) level by microbiosensor is a promising approach for further investigation of the important role of neurotransmitter. The neurotransmitter glutamate has shown in development of brain function including cognition, memory and learning process. Glutamate microbiosensor should serve high sensitivity which able to response for low concentration, high selectivity from interferences, fast and accurate result. Surface teflon encapsulated platinum (Pt) electrode disk design of internal size diameter 50 µm, electropolymerized with poly-o-phenylenediamine (PPD), layered with nafion (Naf), immobilized with glutamate oxidase (GluOx) and cross-linked glutaraldehyde (GA) (Pt50/PPD/Naf/GluOx/GA) has chosen in glutamate detection and performed promising glutamate biosensor using constant potential amperometry (CPA) where Michaelis-Menten for glutamate response, Jmax = 120 µAcm-2, KM= 60 µM and limit of detection (LOD) is 2.5 µM ± 0.5 µM were obtained. The response time was ~9s which is three times faster as compared to Pt50/PPD/GluOx/Ga. The developed glutamate microbiosensor have established an excellent in rejection against ascorbic acid (AA) and other interferences. This electrode designed configuration shows good sensitivity in neutral pH range at pH 7.5. The stability of the electrode was stable until 3 days and kept in cold temperature at 4oC. The reduced enzyme activity on the electrode was due to dissolution of the enzymatic membrane during storage and causing the sensitivity of electrode less stable along the period. In vivo studied shows that data sampling rate of electrode without enzyme (Pt50/PPD/Naf) and with enzyme (Pt50/PPD/Naf/GluOx/GA) were 0.0022 µA and 0.0039 µA, respectively. The ability of glutamate microbiosensor in monitoring the presence of glutamate in the prefrontal cortex of anaesthetized rats was proven.
format	Thesis
qualification_level	Master's degree
author	Hamdan, Siti Kartika
author_facet	Hamdan, Siti Kartika
author_sort	Hamdan, Siti Kartika
title	Development of brain glutamate microbiosensor using platinum (Pt) electrode and application of in vivo / Siti Kartika Hamdan
title_short	Development of brain glutamate microbiosensor using platinum (Pt) electrode and application of in vivo / Siti Kartika Hamdan
title_full	Development of brain glutamate microbiosensor using platinum (Pt) electrode and application of in vivo / Siti Kartika Hamdan
title_fullStr	Development of brain glutamate microbiosensor using platinum (Pt) electrode and application of in vivo / Siti Kartika Hamdan
title_full_unstemmed	Development of brain glutamate microbiosensor using platinum (Pt) electrode and application of in vivo / Siti Kartika Hamdan
title_sort	development of brain glutamate microbiosensor using platinum (pt) electrode and application of in vivo / siti kartika hamdan
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/99136/1/99136.pdf
_version_	1818588001357266944

Development of brain glutamate microbiosensor using platinum (Pt) electrode and application of in vivo / Siti Kartika Hamdan

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