Chemical constituents and biological activities of Artocarpus communis and artocarpus odoratissimus leaves extracts / Clifford Junaidi Kutoi

Chemical constituents and biological activities of Artocarpus communis and artocarpus odoratissimus leaves extracts / Clifford Junaidi Kutoi

Previous studies revealed that Artocarpus species contained several bioactive compounds with potential to provide relief in treatment of diarrhea, wound healing and liver cirrhosis but never been tested for its cytotoxicity properties against liver cell lines to confirm this traditionally medical us...

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Main Author: Junaidi Kutoi, Clifford
Format: Thesis
Language:English
Published: 2016
Online Access:https://ir.uitm.edu.my/id/eprint/99572/1/99572.pdf
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spelling my-uitm-ir.995722024-09-17T03:55:58Z Chemical constituents and biological activities of Artocarpus communis and artocarpus odoratissimus leaves extracts / Clifford Junaidi Kutoi 2016 Junaidi Kutoi, Clifford Previous studies revealed that Artocarpus species contained several bioactive compounds with potential to provide relief in treatment of diarrhea, wound healing and liver cirrhosis but never been tested for its cytotoxicity properties against liver cell lines to confirm this traditionally medical uses with modern scientific test. Therefore it would be interesting to study the cytotoxicity properties of isolated pure compounds against liver cell line such as Hep-G2 together with other bioassays such as antioxidant and antimicrobial. The dried leaves of A. communis and A. odoratissimus were extracted using cold extraction in methanol. Various chromatographic methods such as vacuum liquid chromatography, thin layer chromatography and radial chromatography were used to isolate and purify compounds from the methanolic extracts of both plants. Structural elucidation was accomplished using spectroscopic methods such as ultraviolet, infrared, mass spectroscopy, ID and 2D nuclear magnetic resonance. This study resulted in identifying three flavonoids and three triterpenoids namely squalene (130), l-(2,4- dihydroxyphenyl)-3-[8-hydroxy-2-methyl-2-(4-methyl-3-pentenyl)-2H-l-benzopyran5-yl]-l-propanone (9), 8-(3,6-dimethyl-2-heptenyl)-4',5,7-trihydroxyflavanone (28), 2-geranyl-2',3,4,4'-tetrahydroxydihydrochalcone (11), stigmasterol (132) and ^-sitosterol (131). The methanolic leaves extracts for both A. communis and A. odoratissimus and some of the isolated pure compounds were tested for its antioxidant and antimicrobial activities. Some of these samples demonstarted interesting results in free radical scavenging activity assay; 2-geranyl-2',3,4,4'- tetrahydroxydihydrochalcone showed the most interesting free radical scavenging activity with IC50 value of 5.84 ug/mL as compared to positive control, ascorbic acid with IC50 value of 10.24 ug/mL while, 8-geranyl-4',5,7-trihydroxyflavanone is comparable with control as IC50 recorded at 12.93 ug/mL. Antimicrobial activities based on MIC and MBC revealed some of the tested compounds exhibited strong inhibition activity against Gram-positive bacteria, Staphylococcus aureus, Streptococcus pyogenes and Gram-negative bacteria, Pseudomonas aeruginosa and Escherichia coli with MIC value between 7.03 to 56.25 ug/mL. Pure compound 2- geranyl-2',3,4,4'-tetrahydroxydihydrochalcone demonstrated the most promising inhibition activity against both Gram-positive with MIC value of 7.03 ug/mL 2016 Thesis https://ir.uitm.edu.my/id/eprint/99572/ https://ir.uitm.edu.my/id/eprint/99572/1/99572.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Khong, Heng Yen Syah, Yana Maolana
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Khong, Heng Yen
Syah, Yana Maolana
description Previous studies revealed that Artocarpus species contained several bioactive compounds with potential to provide relief in treatment of diarrhea, wound healing and liver cirrhosis but never been tested for its cytotoxicity properties against liver cell lines to confirm this traditionally medical uses with modern scientific test. Therefore it would be interesting to study the cytotoxicity properties of isolated pure compounds against liver cell line such as Hep-G2 together with other bioassays such as antioxidant and antimicrobial. The dried leaves of A. communis and A. odoratissimus were extracted using cold extraction in methanol. Various chromatographic methods such as vacuum liquid chromatography, thin layer chromatography and radial chromatography were used to isolate and purify compounds from the methanolic extracts of both plants. Structural elucidation was accomplished using spectroscopic methods such as ultraviolet, infrared, mass spectroscopy, ID and 2D nuclear magnetic resonance. This study resulted in identifying three flavonoids and three triterpenoids namely squalene (130), l-(2,4- dihydroxyphenyl)-3-[8-hydroxy-2-methyl-2-(4-methyl-3-pentenyl)-2H-l-benzopyran5-yl]-l-propanone (9), 8-(3,6-dimethyl-2-heptenyl)-4',5,7-trihydroxyflavanone (28), 2-geranyl-2',3,4,4'-tetrahydroxydihydrochalcone (11), stigmasterol (132) and ^-sitosterol (131). The methanolic leaves extracts for both A. communis and A. odoratissimus and some of the isolated pure compounds were tested for its antioxidant and antimicrobial activities. Some of these samples demonstarted interesting results in free radical scavenging activity assay; 2-geranyl-2',3,4,4'- tetrahydroxydihydrochalcone showed the most interesting free radical scavenging activity with IC50 value of 5.84 ug/mL as compared to positive control, ascorbic acid with IC50 value of 10.24 ug/mL while, 8-geranyl-4',5,7-trihydroxyflavanone is comparable with control as IC50 recorded at 12.93 ug/mL. Antimicrobial activities based on MIC and MBC revealed some of the tested compounds exhibited strong inhibition activity against Gram-positive bacteria, Staphylococcus aureus, Streptococcus pyogenes and Gram-negative bacteria, Pseudomonas aeruginosa and Escherichia coli with MIC value between 7.03 to 56.25 ug/mL. Pure compound 2- geranyl-2',3,4,4'-tetrahydroxydihydrochalcone demonstrated the most promising inhibition activity against both Gram-positive with MIC value of 7.03 ug/mL
format Thesis
qualification_level Master's degree
author Junaidi Kutoi, Clifford
spellingShingle Junaidi Kutoi, Clifford
Chemical constituents and biological activities of Artocarpus communis and artocarpus odoratissimus leaves extracts / Clifford Junaidi Kutoi
author_facet Junaidi Kutoi, Clifford
author_sort Junaidi Kutoi, Clifford
title Chemical constituents and biological activities of Artocarpus communis and artocarpus odoratissimus leaves extracts / Clifford Junaidi Kutoi
title_short Chemical constituents and biological activities of Artocarpus communis and artocarpus odoratissimus leaves extracts / Clifford Junaidi Kutoi
title_full Chemical constituents and biological activities of Artocarpus communis and artocarpus odoratissimus leaves extracts / Clifford Junaidi Kutoi
title_fullStr Chemical constituents and biological activities of Artocarpus communis and artocarpus odoratissimus leaves extracts / Clifford Junaidi Kutoi
title_full_unstemmed Chemical constituents and biological activities of Artocarpus communis and artocarpus odoratissimus leaves extracts / Clifford Junaidi Kutoi
title_sort chemical constituents and biological activities of artocarpus communis and artocarpus odoratissimus leaves extracts / clifford junaidi kutoi
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Applied Sciences
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/99572/1/99572.pdf
_version_ 1811769002195681280

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