Sensitivity and specificity testing of a PCR based pharmacogenotyping method for CYP2C9 / Abdullah Qutubuddin Raman

PCR is a special technique used to amplify the DNA sequence of interest. A successful laboratory testing for PCR depends on sensitivity and specificity of PCR method itself. CYP2C9 is an important metabolizing enzyme responsible for 16% of drugs currently available in the market. This enzyme metabol...

Full description

Saved in:

Bibliographic Details
Main Author:	Raman, Abdullah Qutubuddin
Format:	Thesis
Language:	English
Published:	2007
Subjects:	RM Therapeutics Pharmacology RS Pharmacy and materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/99685/1/99685.PDF
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.99685
record_format	uketd_dc
spelling	my-uitm-ir.996852024-08-02T10:19:28Z Sensitivity and specificity testing of a PCR based pharmacogenotyping method for CYP2C9 / Abdullah Qutubuddin Raman 2007 Raman, Abdullah Qutubuddin RM Therapeutics. Pharmacology RS Pharmacy and materia medica PCR is a special technique used to amplify the DNA sequence of interest. A successful laboratory testing for PCR depends on sensitivity and specificity of PCR method itself. CYP2C9 is an important metabolizing enzyme responsible for 16% of drugs currently available in the market. This enzyme metabolized narrow therapeutic index drug such as warfarin, phenytoin and many others. This study aimed to evaluate a PCR pharmacogenotyping test kit that have been developed by the members of Pharmacogenomic Research laboratory. Two aspects of the kit were evaluated, that was the sensitivity and specificity of the test kit. DNA samples were selected randomly from the DNA bank from the laboratory. Ten DNA samples were selected for specificity test. For sensitivity test, 3 different samples with varying concentration of 0.1 ng to 1000 ng with 10 fold dilution were used. The detection limit was found to be 10 ng of DNA concentration. The kit is able to detect genetic variants of CYP2C9 correctly. Thus, it would be useful to clinician in their attempts to personalize medicine for individual patients. 2007 Thesis https://ir.uitm.edu.my/id/eprint/99685/ https://ir.uitm.edu.my/id/eprint/99685/1/99685.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Kek, Teh Lay
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Kek, Teh Lay
topic	RM Therapeutics Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics Pharmacology RS Pharmacy and materia medica Raman, Abdullah Qutubuddin Sensitivity and specificity testing of a PCR based pharmacogenotyping method for CYP2C9 / Abdullah Qutubuddin Raman
description	PCR is a special technique used to amplify the DNA sequence of interest. A successful laboratory testing for PCR depends on sensitivity and specificity of PCR method itself. CYP2C9 is an important metabolizing enzyme responsible for 16% of drugs currently available in the market. This enzyme metabolized narrow therapeutic index drug such as warfarin, phenytoin and many others. This study aimed to evaluate a PCR pharmacogenotyping test kit that have been developed by the members of Pharmacogenomic Research laboratory. Two aspects of the kit were evaluated, that was the sensitivity and specificity of the test kit. DNA samples were selected randomly from the DNA bank from the laboratory. Ten DNA samples were selected for specificity test. For sensitivity test, 3 different samples with varying concentration of 0.1 ng to 1000 ng with 10 fold dilution were used. The detection limit was found to be 10 ng of DNA concentration. The kit is able to detect genetic variants of CYP2C9 correctly. Thus, it would be useful to clinician in their attempts to personalize medicine for individual patients.
format	Thesis
qualification_level	Bachelor degree
author	Raman, Abdullah Qutubuddin
author_facet	Raman, Abdullah Qutubuddin
author_sort	Raman, Abdullah Qutubuddin
title	Sensitivity and specificity testing of a PCR based pharmacogenotyping method for CYP2C9 / Abdullah Qutubuddin Raman
title_short	Sensitivity and specificity testing of a PCR based pharmacogenotyping method for CYP2C9 / Abdullah Qutubuddin Raman
title_full	Sensitivity and specificity testing of a PCR based pharmacogenotyping method for CYP2C9 / Abdullah Qutubuddin Raman
title_fullStr	Sensitivity and specificity testing of a PCR based pharmacogenotyping method for CYP2C9 / Abdullah Qutubuddin Raman
title_full_unstemmed	Sensitivity and specificity testing of a PCR based pharmacogenotyping method for CYP2C9 / Abdullah Qutubuddin Raman
title_sort	sensitivity and specificity testing of a pcr based pharmacogenotyping method for cyp2c9 / abdullah qutubuddin raman
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2007
url	https://ir.uitm.edu.my/id/eprint/99685/1/99685.PDF
_version_	1811769018804076544

Sensitivity and specificity testing of a PCR based pharmacogenotyping method for CYP2C9 / Abdullah Qutubuddin Raman

Similar Items