Development of a PCR method for detection of UGTJAJ polymorphism / Khairul Muhaimin Othman

Irinotecan is a camptothecin analog with potent antitumor activity resulting from the inhibition of topoisomerase. Dose-limiting toxicity of irinotecan includes severe leukopenia, neutropenia and diarrhea. Genetic polymorphisms of UDP glucuronosyltransferase (UGT) lAl, a key metabolizing enzyme of...

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Main Author:	Othman, Khairul Muhaimin
Format:	Thesis
Language:	English
Published:	2007
Subjects:	RM Therapeutics Pharmacology RS Pharmacy and materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/99883/1/99883.PDF
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id	my-uitm-ir.99883
record_format	uketd_dc
spelling	my-uitm-ir.998832024-08-06T17:50:29Z Development of a PCR method for detection of UGTJAJ polymorphism / Khairul Muhaimin Othman 2007 Othman, Khairul Muhaimin RM Therapeutics. Pharmacology RS Pharmacy and materia medica Irinotecan is a camptothecin analog with potent antitumor activity resulting from the inhibition of topoisomerase. Dose-limiting toxicity of irinotecan includes severe leukopenia, neutropenia and diarrhea. Genetic polymorphisms of UDP glucuronosyltransferase (UGT) lAl, a key metabolizing enzyme of irinotecan, are important determinants of individual variations in susceptibility to toxicity. Pharmacogenomic studies of irinotecan toxicity have therefore focused on genetic polymorphisms of the UGTJAJ gene. This project aimed to develop a simple PCR test to identify genetic variants of UGTJAI and mainly focus on UGTJAJ 6 and UGTJAJ 27, single nucleotide polymorphisms in exon 1 of the UGTJAJ gene that are found mainly in Asian. The allele specific PCR amplification was chosen to be studied because it is suitable for the detection of single nucleotide polymorphism within the gene. In this study, method was optimized only for detection of 27 allele but not for 6 allele. Problem with the primer designed for amplification of 6 allele was thought to be the possible reason for not able to optimize the method for 6 allele. Pipetting error also could be one of the reason for unsuccessful amplification process. Further study should be carried out to continue this beneficial research. This PCR method can also be commercialized as a tool for individualization of pharmacotherapy especially anti-cancer therapy once the method is fully optimized and validated as it is cost and time saving in patient management due to reduced effort spent in trial and error procedure. 2007 Thesis https://ir.uitm.edu.my/id/eprint/99883/ https://ir.uitm.edu.my/id/eprint/99883/1/99883.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Salleh, Mohd Zaki
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Salleh, Mohd Zaki
topic	RM Therapeutics Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics Pharmacology RS Pharmacy and materia medica Othman, Khairul Muhaimin Development of a PCR method for detection of UGTJAJ polymorphism / Khairul Muhaimin Othman
description	Irinotecan is a camptothecin analog with potent antitumor activity resulting from the inhibition of topoisomerase. Dose-limiting toxicity of irinotecan includes severe leukopenia, neutropenia and diarrhea. Genetic polymorphisms of UDP glucuronosyltransferase (UGT) lAl, a key metabolizing enzyme of irinotecan, are important determinants of individual variations in susceptibility to toxicity. Pharmacogenomic studies of irinotecan toxicity have therefore focused on genetic polymorphisms of the UGTJAJ gene. This project aimed to develop a simple PCR test to identify genetic variants of UGTJAI and mainly focus on UGTJAJ 6 and UGTJAJ 27, single nucleotide polymorphisms in exon 1 of the UGTJAJ gene that are found mainly in Asian. The allele specific PCR amplification was chosen to be studied because it is suitable for the detection of single nucleotide polymorphism within the gene. In this study, method was optimized only for detection of 27 allele but not for 6 allele. Problem with the primer designed for amplification of 6 allele was thought to be the possible reason for not able to optimize the method for 6 allele. Pipetting error also could be one of the reason for unsuccessful amplification process. Further study should be carried out to continue this beneficial research. This PCR method can also be commercialized as a tool for individualization of pharmacotherapy especially anti-cancer therapy once the method is fully optimized and validated as it is cost and time saving in patient management due to reduced effort spent in trial and error procedure.
format	Thesis
qualification_level	Bachelor degree
author	Othman, Khairul Muhaimin
author_facet	Othman, Khairul Muhaimin
author_sort	Othman, Khairul Muhaimin
title	Development of a PCR method for detection of UGTJAJ polymorphism / Khairul Muhaimin Othman
title_short	Development of a PCR method for detection of UGTJAJ polymorphism / Khairul Muhaimin Othman
title_full	Development of a PCR method for detection of UGTJAJ polymorphism / Khairul Muhaimin Othman
title_fullStr	Development of a PCR method for detection of UGTJAJ polymorphism / Khairul Muhaimin Othman
title_full_unstemmed	Development of a PCR method for detection of UGTJAJ polymorphism / Khairul Muhaimin Othman
title_sort	development of a pcr method for detection of ugtjaj polymorphism / khairul muhaimin othman
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2007
url	https://ir.uitm.edu.my/id/eprint/99883/1/99883.PDF
_version_	1811769042926567424

Development of a PCR method for detection of UGTJAJ polymorphism / Khairul Muhaimin Othman

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