Antimicrobial activity of endophytic fungal extracts (11-L1A, 11-L3, 11-S3) / Mohd Hafiz Ibrahim
The main objective of this project is to identify fungal strains producing biologically active secondary metabolites. The study was performed by growing and extracting endophytic fungal isolates and determining the antimicrobial activity of these isolates for three different conditions (agar. broth...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2007
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/99942/1/99942.PDF |
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| Summary: | The main objective of this project is to identify fungal strains producing biologically active secondary metabolites. The study was performed by growing and extracting endophytic fungal isolates and determining the antimicrobial activity of these isolates for three different conditions (agar. broth static and broth shaking). Three fungal strains (11-L1A, 11-S3 and 11-L3) were cultivated and the cultivation is repeated until pure cultures were obtained. Mycelium from the pure cultures were subjected to the extraction process by using two solvents which is ethyl acetate and butanol. The pure extracts obtained were subjected to antimicrobial assay by using disc diffusion method. Several bacteria and fungi have been used to test against the extracts and the result had been recorded. Data on the fungi (microscopic images) and extracts were collected for their inclusion in a database developed at the Faculty of Pharmacy, UiTM. |
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