Induction of apoptosis by fungal extracts HAB10R12 and HAB14R3 against colon (HCT116) cancer cell lines / Nurhayati Abd Samad

Natural products play an extremely important role as the major source of drugs used for cancer treatment. Endophytes, a new source of natural products, are microorganisms that reside in the living tissues of the host plant and may produce substances that have potential to be used in modern medicine....

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Main Author:	Abd Samad, Nurhayati
Format:	Thesis
Language:	English
Published:	2007
Subjects:	Cancer RS Pharmacy and materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/99947/1/99947.PDF
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id	my-uitm-ir.99947
record_format	uketd_dc
spelling	my-uitm-ir.999472024-08-07T15:02:17Z Induction of apoptosis by fungal extracts HAB10R12 and HAB14R3 against colon (HCT116) cancer cell lines / Nurhayati Abd Samad 2007 Abd Samad, Nurhayati Cancer RM Therapeutics. Pharmacology RS Pharmacy and materia medica Natural products play an extremely important role as the major source of drugs used for cancer treatment. Endophytes, a new source of natural products, are microorganisms that reside in the living tissues of the host plant and may produce substances that have potential to be used in modern medicine. This study aims to determine the cytotoxic activity of endophytic extracts (HAB10R12 and HAB14R3) against colon (HCT116) cancer cells. The mode of cell death elicited by HAB10R12 and HAB14R3 and apoptotic related genes involved in the cell death were also investigated. The endophytic fungi were subcultured on half strength PDA agar and extracted with ethyl acetate. The cytotoxic activities of the extracts against HCT116 were evaluated using the MIT assay. Cell death detection was carried out using Cell Death Detection ELISAPLUS. The results from present study showed that the cytotoxic activity (IC50) of the extracts were 0.055µg/ml for HAB10R12 and 0.056µg/ml for HAB14R3.The results also indicated that the mechanism of cell death elicited by extracts against HCT116 were mainly due to apoptosis. The HAB14R3 exhibited better apoptotic activity when compared to HAB10R12. The study of apoptotic related genes however, was inconclusive. PCR methods needs to be optimised to confirm the expression of gene related to the apoptotic pathway. In future, more studies also should be conducted with pure compounds of HAB10R12 and HAB14R3 in the future to discover the other potentials possess by these endophytic fungi. 2007 Thesis https://ir.uitm.edu.my/id/eprint/99947/ https://ir.uitm.edu.my/id/eprint/99947/1/99947.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Ramasamy, Kalavathy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ramasamy, Kalavathy
topic	Cancer Cancer RS Pharmacy and materia medica
spellingShingle	Cancer Cancer RS Pharmacy and materia medica Abd Samad, Nurhayati Induction of apoptosis by fungal extracts HAB10R12 and HAB14R3 against colon (HCT116) cancer cell lines / Nurhayati Abd Samad
description	Natural products play an extremely important role as the major source of drugs used for cancer treatment. Endophytes, a new source of natural products, are microorganisms that reside in the living tissues of the host plant and may produce substances that have potential to be used in modern medicine. This study aims to determine the cytotoxic activity of endophytic extracts (HAB10R12 and HAB14R3) against colon (HCT116) cancer cells. The mode of cell death elicited by HAB10R12 and HAB14R3 and apoptotic related genes involved in the cell death were also investigated. The endophytic fungi were subcultured on half strength PDA agar and extracted with ethyl acetate. The cytotoxic activities of the extracts against HCT116 were evaluated using the MIT assay. Cell death detection was carried out using Cell Death Detection ELISAPLUS. The results from present study showed that the cytotoxic activity (IC50) of the extracts were 0.055µg/ml for HAB10R12 and 0.056µg/ml for HAB14R3.The results also indicated that the mechanism of cell death elicited by extracts against HCT116 were mainly due to apoptosis. The HAB14R3 exhibited better apoptotic activity when compared to HAB10R12. The study of apoptotic related genes however, was inconclusive. PCR methods needs to be optimised to confirm the expression of gene related to the apoptotic pathway. In future, more studies also should be conducted with pure compounds of HAB10R12 and HAB14R3 in the future to discover the other potentials possess by these endophytic fungi.
format	Thesis
qualification_level	Bachelor degree
author	Abd Samad, Nurhayati
author_facet	Abd Samad, Nurhayati
author_sort	Abd Samad, Nurhayati
title	Induction of apoptosis by fungal extracts HAB10R12 and HAB14R3 against colon (HCT116) cancer cell lines / Nurhayati Abd Samad
title_short	Induction of apoptosis by fungal extracts HAB10R12 and HAB14R3 against colon (HCT116) cancer cell lines / Nurhayati Abd Samad
title_full	Induction of apoptosis by fungal extracts HAB10R12 and HAB14R3 against colon (HCT116) cancer cell lines / Nurhayati Abd Samad
title_fullStr	Induction of apoptosis by fungal extracts HAB10R12 and HAB14R3 against colon (HCT116) cancer cell lines / Nurhayati Abd Samad
title_full_unstemmed	Induction of apoptosis by fungal extracts HAB10R12 and HAB14R3 against colon (HCT116) cancer cell lines / Nurhayati Abd Samad
title_sort	induction of apoptosis by fungal extracts hab10r12 and hab14r3 against colon (hct116) cancer cell lines / nurhayati abd samad
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2007
url	https://ir.uitm.edu.my/id/eprint/99947/1/99947.PDF
_version_	1811769065038938112

Induction of apoptosis by fungal extracts HAB10R12 and HAB14R3 against colon (HCT116) cancer cell lines / Nurhayati Abd Samad

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