Antibacterial and antihypertensive properties of crude extract of in vitro callus culture of muskmelon (cucumis melo l.)

Muskmelon or Cucumis melo L. is one of the melon families that are widely consumed in many countries. The plant’s ability to adapt to many climate condition makes it available in the market throughout the year. Phytochemicals is one of the important components in plant. In this study, the methanolic...

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Main Author:	Nurul Ashikin, Abdul Karim
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QK Botany
Online Access:	http://umpir.ump.edu.my/id/eprint/10763/1/CD8267_ANTIBACTERIAL%20AND%20ANTIHYPERTENSIVE%20PROPERTIES%20OF%20CRUDE%20EXTRACT%20OF%20IN%20VITRO.pdf
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spelling	my-ump-ir.107632023-04-04T02:56:16Z Antibacterial and antihypertensive properties of crude extract of in vitro callus culture of muskmelon (cucumis melo l.) 2014-04 Nurul Ashikin, Abdul Karim QK Botany Muskmelon or Cucumis melo L. is one of the melon families that are widely consumed in many countries. The plant’s ability to adapt to many climate condition makes it available in the market throughout the year. Phytochemicals is one of the important components in plant. In this study, the methanolic extract of callus from muskmelon were studied and tested for antihypertensive and antibacterial activities. The explants were chosen from plant part; leaf, stem and fruit according to the highest total phenolic content where the leaf part showed the highest content. The calli were induced from the explant in the medium with plant growth regulators (PGRs) at a concentration ranging from 0 to 2.0 mg/L. Murashige and Skoog (MS) medium supplemented with PGRs: Zeatin (Z) 1.5 mg/L with 2,4-Dichlorophenoxyaceticacid (2,4-D) 1.5 mg/L, 6-Benzylaminopurine (BAP) 0.5 mg/L with 2,4D 1.5 mg/L, Indole-3-Butyricacid (IBA) alone 1.5 mg/L and 2,4-D alone 1.5 mg/L have successfully induced viable callus for extraction. The four weeks old calli were chosen for methanolic extraction because of its high total phenolic (TP) and total flavonoid (TF) content. It was found that C4 contained the highest amount of TP and C3 showed highest TF. The methanolic extracts were tested for antibacterial and antihypertensive activity. Three Gram Negative bacteria Escherichia coli (ATCC 10536), Salmonella typhi (ATCC 1331), Pseudomonas aeroginosa (ATCC 1542), and three Gram Positive bacteria Enterococcus faecalis (ATCC 14506), Staphylococcus aureus (ATCC baa1026), , and Bacillus subtilis (ATCC 11774), were used for antibacterial test in this study. Only the C3 and C4 extracts were able to inhibit bacterial growth as measured by inhibition zone and minimal inhibitory concentration. While in antihypertensive test, the C4 extract showed the highest inhibition rate (70.32%) against angiotensin converting enzyme and the extract from C3 showed the lowest (50.78 %) inhibition. The phytochemical profile of the extracts was determined by HPLC where the presence of phenolics and flavonoids were detected in some of the extracts 2014-04 Thesis http://umpir.ump.edu.my/id/eprint/10763/ http://umpir.ump.edu.my/id/eprint/10763/1/CD8267_ANTIBACTERIAL%20AND%20ANTIHYPERTENSIVE%20PROPERTIES%20OF%20CRUDE%20EXTRACT%20OF%20IN%20VITRO.pdf pdf en public masters Universiti Malaysia Pahang Faculty Of Industrial Science and Technology Govindan, Natanamurugaraj
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
advisor	Govindan, Natanamurugaraj
topic	QK Botany
spellingShingle	QK Botany Nurul Ashikin, Abdul Karim Antibacterial and antihypertensive properties of crude extract of in vitro callus culture of muskmelon (cucumis melo l.)
description	Muskmelon or Cucumis melo L. is one of the melon families that are widely consumed in many countries. The plant’s ability to adapt to many climate condition makes it available in the market throughout the year. Phytochemicals is one of the important components in plant. In this study, the methanolic extract of callus from muskmelon were studied and tested for antihypertensive and antibacterial activities. The explants were chosen from plant part; leaf, stem and fruit according to the highest total phenolic content where the leaf part showed the highest content. The calli were induced from the explant in the medium with plant growth regulators (PGRs) at a concentration ranging from 0 to 2.0 mg/L. Murashige and Skoog (MS) medium supplemented with PGRs: Zeatin (Z) 1.5 mg/L with 2,4-Dichlorophenoxyaceticacid (2,4-D) 1.5 mg/L, 6-Benzylaminopurine (BAP) 0.5 mg/L with 2,4D 1.5 mg/L, Indole-3-Butyricacid (IBA) alone 1.5 mg/L and 2,4-D alone 1.5 mg/L have successfully induced viable callus for extraction. The four weeks old calli were chosen for methanolic extraction because of its high total phenolic (TP) and total flavonoid (TF) content. It was found that C4 contained the highest amount of TP and C3 showed highest TF. The methanolic extracts were tested for antibacterial and antihypertensive activity. Three Gram Negative bacteria Escherichia coli (ATCC 10536), Salmonella typhi (ATCC 1331), Pseudomonas aeroginosa (ATCC 1542), and three Gram Positive bacteria Enterococcus faecalis (ATCC 14506), Staphylococcus aureus (ATCC baa1026), , and Bacillus subtilis (ATCC 11774), were used for antibacterial test in this study. Only the C3 and C4 extracts were able to inhibit bacterial growth as measured by inhibition zone and minimal inhibitory concentration. While in antihypertensive test, the C4 extract showed the highest inhibition rate (70.32%) against angiotensin converting enzyme and the extract from C3 showed the lowest (50.78 %) inhibition. The phytochemical profile of the extracts was determined by HPLC where the presence of phenolics and flavonoids were detected in some of the extracts
format	Thesis
qualification_level	Master's degree
author	Nurul Ashikin, Abdul Karim
author_facet	Nurul Ashikin, Abdul Karim
author_sort	Nurul Ashikin, Abdul Karim
title	Antibacterial and antihypertensive properties of crude extract of in vitro callus culture of muskmelon (cucumis melo l.)
title_short	Antibacterial and antihypertensive properties of crude extract of in vitro callus culture of muskmelon (cucumis melo l.)
title_full	Antibacterial and antihypertensive properties of crude extract of in vitro callus culture of muskmelon (cucumis melo l.)
title_fullStr	Antibacterial and antihypertensive properties of crude extract of in vitro callus culture of muskmelon (cucumis melo l.)
title_full_unstemmed	Antibacterial and antihypertensive properties of crude extract of in vitro callus culture of muskmelon (cucumis melo l.)
title_sort	antibacterial and antihypertensive properties of crude extract of in vitro callus culture of muskmelon (cucumis melo l.)
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty Of Industrial Science and Technology
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/10763/1/CD8267_ANTIBACTERIAL%20AND%20ANTIHYPERTENSIVE%20PROPERTIES%20OF%20CRUDE%20EXTRACT%20OF%20IN%20VITRO.pdf
_version_	1783731932688809984

Antibacterial and antihypertensive properties of crude extract of in vitro callus culture of muskmelon (cucumis melo l.)

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