Chemical composition, antioxidant and antibacterial activity of essential oil from leaf of syzygium polyanthum (wight) walp.

Syzygium polyanthum (Wight) Walp. is a plant widely consumed as raw vegetables or used in culinary in Malaysia. This study was performed to determine the chemical composition, antioxidant and antibacterial activity of Syzygium polyanthum essential oil from Malaysia. The essential oil was extracted f...

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Main Author: Nur Amalina, Ahmad
Format: Thesis
Language:English
Published: 2014
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Online Access:http://umpir.ump.edu.my/id/eprint/10767/1/CD8270_CHEMICAL%20COMPOSITION%2C%20ANTIOXIDANT%20AND%20ANTIBACTERIAL%20ACTIVITY%20OF%20ESSENTIAL%20OIL%20FROM%20LEAF%20OF%20SYZYGIUM.pdf
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spelling my-ump-ir.107672023-05-17T07:27:36Z Chemical composition, antioxidant and antibacterial activity of essential oil from leaf of syzygium polyanthum (wight) walp. 2014-04 Nur Amalina, Ahmad QP Physiology Syzygium polyanthum (Wight) Walp. is a plant widely consumed as raw vegetables or used in culinary in Malaysia. This study was performed to determine the chemical composition, antioxidant and antibacterial activity of Syzygium polyanthum essential oil from Malaysia. The essential oil was extracted from the leaf by hydrodistillation method. The percentage of the essential oils obtained, for the fresh and the dried leaf were 0.67 % and 1.50 %, respectively. Analysis by GCMS revealed a total of 26 and 29 compounds, representing 71.71 % and 90.92 % of the overall compositions, were detected in the fresh and the dried leaf essential oils. Both oils were characterized by high amount of monoterpene hydrocarbons with α-pinene was the major compounds for the fresh leaf (28.78 %) and the dried leaf (34.15 %). The antioxidant activities of the fresh and dried leaf oils were evaluated using three different assay; DPPH free radical scavenging, β-carotene bleaching and ferrous-ion chelating. Results showed that the oils exhibited a potential antioxidant activity. The antibacterial activity was evaluated using the disc diffusion and microdilution methods against three gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis) and three gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhii). The oils exhibited antibacterial activity against all of the tested bacteria. The minimum inhibitory concentration (MIC) values for the fresh leaf oil were in the range of 0.03 mg/ml to 0.25 mg/ml while the MIC values for the dried leaf oil were in the range of 0.03 mg/ml to 0.12 mg/ml. The chemical composition, antioxidant and antibacterial activity of essential oil of S. polyanthum from Malaysia is presented here for the first time 2014-04 Thesis http://umpir.ump.edu.my/id/eprint/10767/ http://umpir.ump.edu.my/id/eprint/10767/1/CD8270_CHEMICAL%20COMPOSITION%2C%20ANTIOXIDANT%20AND%20ANTIBACTERIAL%20ACTIVITY%20OF%20ESSENTIAL%20OIL%20FROM%20LEAF%20OF%20SYZYGIUM.pdf pdf en public masters Universiti Malaysia Pahang Faculty Of Industrial Science and Technology Govindan, Natanamurugaraj
institution Universiti Malaysia Pahang Al-Sultan Abdullah
collection UMPSA Institutional Repository
language English
advisor Govindan, Natanamurugaraj
topic QP Physiology
spellingShingle QP Physiology
Nur Amalina, Ahmad
Chemical composition, antioxidant and antibacterial activity of essential oil from leaf of syzygium polyanthum (wight) walp.
description Syzygium polyanthum (Wight) Walp. is a plant widely consumed as raw vegetables or used in culinary in Malaysia. This study was performed to determine the chemical composition, antioxidant and antibacterial activity of Syzygium polyanthum essential oil from Malaysia. The essential oil was extracted from the leaf by hydrodistillation method. The percentage of the essential oils obtained, for the fresh and the dried leaf were 0.67 % and 1.50 %, respectively. Analysis by GCMS revealed a total of 26 and 29 compounds, representing 71.71 % and 90.92 % of the overall compositions, were detected in the fresh and the dried leaf essential oils. Both oils were characterized by high amount of monoterpene hydrocarbons with α-pinene was the major compounds for the fresh leaf (28.78 %) and the dried leaf (34.15 %). The antioxidant activities of the fresh and dried leaf oils were evaluated using three different assay; DPPH free radical scavenging, β-carotene bleaching and ferrous-ion chelating. Results showed that the oils exhibited a potential antioxidant activity. The antibacterial activity was evaluated using the disc diffusion and microdilution methods against three gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis) and three gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhii). The oils exhibited antibacterial activity against all of the tested bacteria. The minimum inhibitory concentration (MIC) values for the fresh leaf oil were in the range of 0.03 mg/ml to 0.25 mg/ml while the MIC values for the dried leaf oil were in the range of 0.03 mg/ml to 0.12 mg/ml. The chemical composition, antioxidant and antibacterial activity of essential oil of S. polyanthum from Malaysia is presented here for the first time
format Thesis
qualification_level Master's degree
author Nur Amalina, Ahmad
author_facet Nur Amalina, Ahmad
author_sort Nur Amalina, Ahmad
title Chemical composition, antioxidant and antibacterial activity of essential oil from leaf of syzygium polyanthum (wight) walp.
title_short Chemical composition, antioxidant and antibacterial activity of essential oil from leaf of syzygium polyanthum (wight) walp.
title_full Chemical composition, antioxidant and antibacterial activity of essential oil from leaf of syzygium polyanthum (wight) walp.
title_fullStr Chemical composition, antioxidant and antibacterial activity of essential oil from leaf of syzygium polyanthum (wight) walp.
title_full_unstemmed Chemical composition, antioxidant and antibacterial activity of essential oil from leaf of syzygium polyanthum (wight) walp.
title_sort chemical composition, antioxidant and antibacterial activity of essential oil from leaf of syzygium polyanthum (wight) walp.
granting_institution Universiti Malaysia Pahang
granting_department Faculty Of Industrial Science and Technology
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/10767/1/CD8270_CHEMICAL%20COMPOSITION%2C%20ANTIOXIDANT%20AND%20ANTIBACTERIAL%20ACTIVITY%20OF%20ESSENTIAL%20OIL%20FROM%20LEAF%20OF%20SYZYGIUM.pdf
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