Application of enzyme-producing bacteria for municipal solid waste biodegradation

Population growth with increasing consumption levels leads to abundant waste in Kuantan. Jabor landfill, commonly known as Kuantan landfill, receives more than 500 tons of waste per day with a composition of 60% of domestic waste and 40% of commercial waste. The composition of domestic waste were or...

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Main Author:	Nailah, Sa’adah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/12615/1/Application%20of%20enzyme-producing%20bacteria%20for%20municipal%20solid%20waste%20biodegradation.pdf
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spelling	my-ump-ir.126152023-04-10T07:25:46Z Application of enzyme-producing bacteria for municipal solid waste biodegradation 2015-08 Nailah, Sa’adah Q Science (General) Population growth with increasing consumption levels leads to abundant waste in Kuantan. Jabor landfill, commonly known as Kuantan landfill, receives more than 500 tons of waste per day with a composition of 60% of domestic waste and 40% of commercial waste. The composition of domestic waste were organic waste, green waste, mixed paper, plastic, textile, ferrous, glass, rubber and leather, and others. Meanwhile, the compositions of commercial waste are food, plastic, yard waste, paper, cardboard, textile, glass, non-ferrous, rubber, wood, ferrous metals. Landfill system always produces leachate. This waste contains many types of bacteria with the potential to degrade the waste compound. Screening tests with selective media demonstrated the ability of bacteria to produce amylase, lipase, protease and cellulase enzymes. These enzymes are needed to accelerate the molecules breakdown of municipal solid waste in the biodegradation process. Samples for isolation of bacteria were taken from different places, namely landfill soil and leachate. Identification of bacteria was conducted using Gen III microplate BIOLOG microbial identification system. They were Bacillus amyloliquefaciens, Bacillus ruris, Bacillus licheniformis, Bacillus subtilis and Kocuria varians. There were 4 different treatments: composting pile without bacteria (T0), composting pile with inoculation of amylase and protease producing bacteria (T1), composting with inoculation of lipase and cellulase producing bacteria (T2), and composting with inoculation all enzyme-producing bacteria (T3). The result of biodegradation process of T3 treatment reached the highest temperature (53° C) with the longest thermophilic phase compared to other treatments. The lower value of C/N ratio, the more stable the level of maturity of compost. The lowest of C/N ratio value was T3 (10%). T3 treatment compared with other treatments can increase as much as 27% content of nitrogen, 67% of phosphorus and 33% of potassium. All the treatments with bacterial inoculation (T1, T2, and T3) are able to reduce the content of heavy metals (Fe, Zn, Cu) on municipal solid waste biodegradation. It can be concluded that the inoculation of potential enzyme-producing of bacteria on municipal solid waste biodegradation is effective to increase the nutrient content and decrease the heavy metals. 2015-08 Thesis http://umpir.ump.edu.my/id/eprint/12615/ http://umpir.ump.edu.my/id/eprint/12615/1/Application%20of%20enzyme-producing%20bacteria%20for%20municipal%20solid%20waste%20biodegradation.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Industrial Science and Technology Wan Ishak, Wan Mohd Faizal
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
advisor	Wan Ishak, Wan Mohd Faizal
topic	Q Science (General)
spellingShingle	Q Science (General) Nailah, Sa’adah Application of enzyme-producing bacteria for municipal solid waste biodegradation
description	Population growth with increasing consumption levels leads to abundant waste in Kuantan. Jabor landfill, commonly known as Kuantan landfill, receives more than 500 tons of waste per day with a composition of 60% of domestic waste and 40% of commercial waste. The composition of domestic waste were organic waste, green waste, mixed paper, plastic, textile, ferrous, glass, rubber and leather, and others. Meanwhile, the compositions of commercial waste are food, plastic, yard waste, paper, cardboard, textile, glass, non-ferrous, rubber, wood, ferrous metals. Landfill system always produces leachate. This waste contains many types of bacteria with the potential to degrade the waste compound. Screening tests with selective media demonstrated the ability of bacteria to produce amylase, lipase, protease and cellulase enzymes. These enzymes are needed to accelerate the molecules breakdown of municipal solid waste in the biodegradation process. Samples for isolation of bacteria were taken from different places, namely landfill soil and leachate. Identification of bacteria was conducted using Gen III microplate BIOLOG microbial identification system. They were Bacillus amyloliquefaciens, Bacillus ruris, Bacillus licheniformis, Bacillus subtilis and Kocuria varians. There were 4 different treatments: composting pile without bacteria (T0), composting pile with inoculation of amylase and protease producing bacteria (T1), composting with inoculation of lipase and cellulase producing bacteria (T2), and composting with inoculation all enzyme-producing bacteria (T3). The result of biodegradation process of T3 treatment reached the highest temperature (53° C) with the longest thermophilic phase compared to other treatments. The lower value of C/N ratio, the more stable the level of maturity of compost. The lowest of C/N ratio value was T3 (10%). T3 treatment compared with other treatments can increase as much as 27% content of nitrogen, 67% of phosphorus and 33% of potassium. All the treatments with bacterial inoculation (T1, T2, and T3) are able to reduce the content of heavy metals (Fe, Zn, Cu) on municipal solid waste biodegradation. It can be concluded that the inoculation of potential enzyme-producing of bacteria on municipal solid waste biodegradation is effective to increase the nutrient content and decrease the heavy metals.
format	Thesis
qualification_level	Master's degree
author	Nailah, Sa’adah
author_facet	Nailah, Sa’adah
author_sort	Nailah, Sa’adah
title	Application of enzyme-producing bacteria for municipal solid waste biodegradation
title_short	Application of enzyme-producing bacteria for municipal solid waste biodegradation
title_full	Application of enzyme-producing bacteria for municipal solid waste biodegradation
title_fullStr	Application of enzyme-producing bacteria for municipal solid waste biodegradation
title_full_unstemmed	Application of enzyme-producing bacteria for municipal solid waste biodegradation
title_sort	application of enzyme-producing bacteria for municipal solid waste biodegradation
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Industrial Science and Technology
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/12615/1/Application%20of%20enzyme-producing%20bacteria%20for%20municipal%20solid%20waste%20biodegradation.pdf
_version_	1783731967934595072

Application of enzyme-producing bacteria for municipal solid waste biodegradation

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