Synergistic ferulic acid production from banana stem waste by co-culture

Ferulic acid (FA) production using microbes has currently become extensive owing to its antioxidant properties. Both fungi and bacteria have been used in producing ferulic acid from agricultural waste. However, less work has been done on bacteria compared to fungi. Therefore, an appropriate bacteria...

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Main Author:	Kamaliah, Abdul Samad
Format:	Thesis
Language:	English
Published:	2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/19559/19/Synergistic%20ferulic%20acid%20production%20from%20banana%20stem%20waste%20by%20co-culture.pdf
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spelling	my-ump-ir.195592022-01-04T23:38:34Z Synergistic ferulic acid production from banana stem waste by co-culture 2017-05 Kamaliah, Abdul Samad TP Chemical technology Ferulic acid (FA) production using microbes has currently become extensive owing to its antioxidant properties. Both fungi and bacteria have been used in producing ferulic acid from agricultural waste. However, less work has been done on bacteria compared to fungi. Therefore, an appropriate bacterial co-culture fermentation technique using banana stem waste (BSW) as substrate was implemented to improve FA production. Soil microbes were isolated and preliminary study was performed to select the best bacterial strain. Subsequently, 26 type of bacterial co-cultures were formed and evaluated their ability dependent on FA production. Optimization of the FA production was conducted by employing response surface methodology using the selected co-culture as inoculum. A kinetic study was carried out to determine kinetic constants using Michaelis-Menten equation. Meanwhile, the enzyme assay was performed to investigate the mechanism release of FA by enzymatic hydrolysis. Ferulic acid production was accomplished through submerged fermentation and the sample was analyzed by HPLC to quantify the FA content. The result found that 5 out of 46 isolated bacterial strains were efficient in releasing FA from BSW. However, the highest production of FA was observed in co-culture A (Bacillus cereus CCM 2010, Bacillus pumilus SAFR-032 and Bacillus thuringiensis Bt407), followed by co-culture B (Bacillus cereus CCM 2010, and Bacillus thuringiensis Bt407). In improving the fermentation process, screening by fractional factorial design was performed and the result obtained presented four factors including pH, rotation rate, type of co-culture, and volume of inoculum have significant effects on FA production. Furthermore, rotation rate at 150 rpm and volume of inoculum of 5 % were found to be optimum in increasing FA yield up to 510.24 mg/kg within 24 h using bacterial co-culture B as an inoculum. Meanwhile, the kinetic constant of 2017-05 Thesis http://umpir.ump.edu.my/id/eprint/19559/ http://umpir.ump.edu.my/id/eprint/19559/19/Synergistic%20ferulic%20acid%20production%20from%20banana%20stem%20waste%20by%20co-culture.pdf pdf en public phd doctoral Universiti Malaysia Pahang Faculty of Chemical and Natural Resources Engineering
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Kamaliah, Abdul Samad Synergistic ferulic acid production from banana stem waste by co-culture
description	Ferulic acid (FA) production using microbes has currently become extensive owing to its antioxidant properties. Both fungi and bacteria have been used in producing ferulic acid from agricultural waste. However, less work has been done on bacteria compared to fungi. Therefore, an appropriate bacterial co-culture fermentation technique using banana stem waste (BSW) as substrate was implemented to improve FA production. Soil microbes were isolated and preliminary study was performed to select the best bacterial strain. Subsequently, 26 type of bacterial co-cultures were formed and evaluated their ability dependent on FA production. Optimization of the FA production was conducted by employing response surface methodology using the selected co-culture as inoculum. A kinetic study was carried out to determine kinetic constants using Michaelis-Menten equation. Meanwhile, the enzyme assay was performed to investigate the mechanism release of FA by enzymatic hydrolysis. Ferulic acid production was accomplished through submerged fermentation and the sample was analyzed by HPLC to quantify the FA content. The result found that 5 out of 46 isolated bacterial strains were efficient in releasing FA from BSW. However, the highest production of FA was observed in co-culture A (Bacillus cereus CCM 2010, Bacillus pumilus SAFR-032 and Bacillus thuringiensis Bt407), followed by co-culture B (Bacillus cereus CCM 2010, and Bacillus thuringiensis Bt407). In improving the fermentation process, screening by fractional factorial design was performed and the result obtained presented four factors including pH, rotation rate, type of co-culture, and volume of inoculum have significant effects on FA production. Furthermore, rotation rate at 150 rpm and volume of inoculum of 5 % were found to be optimum in increasing FA yield up to 510.24 mg/kg within 24 h using bacterial co-culture B as an inoculum. Meanwhile, the kinetic constant of
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Kamaliah, Abdul Samad
author_facet	Kamaliah, Abdul Samad
author_sort	Kamaliah, Abdul Samad
title	Synergistic ferulic acid production from banana stem waste by co-culture
title_short	Synergistic ferulic acid production from banana stem waste by co-culture
title_full	Synergistic ferulic acid production from banana stem waste by co-culture
title_fullStr	Synergistic ferulic acid production from banana stem waste by co-culture
title_full_unstemmed	Synergistic ferulic acid production from banana stem waste by co-culture
title_sort	synergistic ferulic acid production from banana stem waste by co-culture
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Chemical and Natural Resources Engineering
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/19559/19/Synergistic%20ferulic%20acid%20production%20from%20banana%20stem%20waste%20by%20co-culture.pdf
_version_	1783732040810627072

Synergistic ferulic acid production from banana stem waste by co-culture

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