Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus

Benzyl acetone, a carboxylic derivative constitute a considerable percentage of the overall yield of essential oil extracted from Aquilaria malaccensis (agarwood) and thus, considered as one of the major constituents of agarwood oil. This study aimed at isolating benzyl acetone from essential oil of...

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Main Author:	Norul Amalina, Sabri
Format:	Thesis
Language:	English
Published:	2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/20522/1/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus.wm.pdf
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spelling	my-ump-ir.205222023-05-09T07:30:02Z Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus 2017 Norul Amalina, Sabri TP Chemical technology Benzyl acetone, a carboxylic derivative constitute a considerable percentage of the overall yield of essential oil extracted from Aquilaria malaccensis (agarwood) and thus, considered as one of the major constituents of agarwood oil. This study aimed at isolating benzyl acetone from essential oil of A. malaccensis using preparative GC (prep-GC); studying the fate of benzyl acetone when transformed using selected microorganisms commonly found in environment; developing efficient biotransformation system by optimizing parameters namely initial substrate and glucose concentration, initial pH and temperature of reaction. Essential oil of A. malaccensis was extracted using Microwave-Assisted Extraction (MAE) and preparative GC (prep-GC) was used to isolate benzyl acetone. Identity of isolated benzyl acetone was confirmed by comparing the peak to that of commercial benzyl acetone using GC-MS. The whole cells of Saccharomyces cerevisiae, Aspergillus niger, Pseudomonas aeruginosa, Enterococcusfaecalis and Bacillus cereus were used for biotransformation of commercial benzyl acetone in this study. GC-MS was used to identify and quantify biotransformation product. Reduction of benzyl acetone to 4-phenyl-2-butanol occurred in all experiments using these five microorganisms. The optimum condition for the highest biotransformation activity using S. cerevisiae: 34.01 % conversion in 0.1 g/L benzyl acetone, 20 g/L glucose, pH 7.5 and 30°C; A. niger: 94.33 % conversion in 0.15 g/L benzyl acetone, 50 g/L glucose, pH 7.5 and 27°C; P. aeruginosa: 98.27 % conversion in 0.1 g/L benzyl acetone, 20 g/L glucose, pH 7.0 and 30°C; E.faecalis: 7.17 % conversion in 0.15 g/L benzyl acetone, 40 g/L glucose, pH 7.0 and 37°C; B. cereus: 9.84 % conversion in 0.15 gIL, 40 g/L glucose, pH 7.0 and 40°C. Biotransformation of benzyl acetone resulted in formation of 4-phenyl-2-butanol which is not naturally found in agarwood oil. P. aeruginosa was proven to be the most efficient biocatalyst for biotransformation of benzyl acetone to 4-phenyl-2-butanol, followed by A. niger, S. cerevisiae, E. faecalis and B. cereus. 2017 Thesis http://umpir.ump.edu.my/id/eprint/20522/ http://umpir.ump.edu.my/id/eprint/20522/1/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus.wm.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Industrial Sciences and Technology
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Norul Amalina, Sabri Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
description	Benzyl acetone, a carboxylic derivative constitute a considerable percentage of the overall yield of essential oil extracted from Aquilaria malaccensis (agarwood) and thus, considered as one of the major constituents of agarwood oil. This study aimed at isolating benzyl acetone from essential oil of A. malaccensis using preparative GC (prep-GC); studying the fate of benzyl acetone when transformed using selected microorganisms commonly found in environment; developing efficient biotransformation system by optimizing parameters namely initial substrate and glucose concentration, initial pH and temperature of reaction. Essential oil of A. malaccensis was extracted using Microwave-Assisted Extraction (MAE) and preparative GC (prep-GC) was used to isolate benzyl acetone. Identity of isolated benzyl acetone was confirmed by comparing the peak to that of commercial benzyl acetone using GC-MS. The whole cells of Saccharomyces cerevisiae, Aspergillus niger, Pseudomonas aeruginosa, Enterococcusfaecalis and Bacillus cereus were used for biotransformation of commercial benzyl acetone in this study. GC-MS was used to identify and quantify biotransformation product. Reduction of benzyl acetone to 4-phenyl-2-butanol occurred in all experiments using these five microorganisms. The optimum condition for the highest biotransformation activity using S. cerevisiae: 34.01 % conversion in 0.1 g/L benzyl acetone, 20 g/L glucose, pH 7.5 and 30°C; A. niger: 94.33 % conversion in 0.15 g/L benzyl acetone, 50 g/L glucose, pH 7.5 and 27°C; P. aeruginosa: 98.27 % conversion in 0.1 g/L benzyl acetone, 20 g/L glucose, pH 7.0 and 30°C; E.faecalis: 7.17 % conversion in 0.15 g/L benzyl acetone, 40 g/L glucose, pH 7.0 and 37°C; B. cereus: 9.84 % conversion in 0.15 gIL, 40 g/L glucose, pH 7.0 and 40°C. Biotransformation of benzyl acetone resulted in formation of 4-phenyl-2-butanol which is not naturally found in agarwood oil. P. aeruginosa was proven to be the most efficient biocatalyst for biotransformation of benzyl acetone to 4-phenyl-2-butanol, followed by A. niger, S. cerevisiae, E. faecalis and B. cereus.
format	Thesis
qualification_level	Master's degree
author	Norul Amalina, Sabri
author_facet	Norul Amalina, Sabri
author_sort	Norul Amalina, Sabri
title	Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
title_short	Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
title_full	Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
title_fullStr	Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
title_full_unstemmed	Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
title_sort	biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Industrial Sciences and Technology
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/20522/1/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus.wm.pdf
_version_	1783732051898269696

Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus

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