Antimicrobial activities of the crude extracts and electrospun nanofibers containing rhodomyrtus tomentosa (kemunting) roots

Traditional medicinal plants can be used to treat chronic and infectious diseases. As a result, the practice of incorporating medicinal plant extracts into polymeric solutions to produce bioactive electrospun nanofibers is rising progressively. In this study Rhodomyrtus tomentosa roots were extracte...

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Main Author:	Zeyohanness, Senait Sileshi
Format:	Thesis
Language:	English
Published:	2018
Subjects:	RV Botanic Thomsonian and eclectic medicine
Online Access:	http://umpir.ump.edu.my/id/eprint/24714/1/Antimicrobial%20activities%20of%20the%20crude%20extracts%20and%20electrospun%20nanofibers%20containing%20rhodomyrtus%20tomentosa%20%28kemunting%29%20roots.wm.pdf
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spelling	my-ump-ir.247142023-04-10T07:09:22Z Antimicrobial activities of the crude extracts and electrospun nanofibers containing rhodomyrtus tomentosa (kemunting) roots 2018-05 Zeyohanness, Senait Sileshi RV Botanic, Thomsonian, and eclectic medicine Traditional medicinal plants can be used to treat chronic and infectious diseases. As a result, the practice of incorporating medicinal plant extracts into polymeric solutions to produce bioactive electrospun nanofibers is rising progressively. In this study Rhodomyrtus tomentosa roots were extracted with hexane, chloroform, ethyl acetate and methanol. Preliminary phytochemical analysis was done by using ultrahighperformance liquid chromatographyquadrupole time-off-light/mass spectrometry (UPLC QToF/MS) method and antimicrobial activity was evaluated against common human pathogenic microorganisms, two Gramnegative bacterial strains (Escherichia coli, Pseudomonas aeruginosa), two Grampositive bacterial strains (Bacillus subtilis, Enterococcus faecalis.) and one fungal strain (Aspergillus niger) by using disk diffusion method and zone of inhibition was determined. The extract with better activity, ethyl acetate extract (EAE), was incorporated with 10% Polyvinyl alcohol (PVA) (W/V) solution at different concentrations (0.25, 0.5, 1.5 and 2.5% W/V) and Ethyl acetate extract of Rhodomyrtus tomentosa roots incorporated in 10% PVA (RTE/PVA) nanofiber were fabricated using electrospinning process. Morphology of the extract loaded nanofibers were characterized using FESEM, FTIR and XRD spectrums. Antimicrobial activity of the electrospun nanofibers was evaluated using same procedures followed during the analysis of all extracts. Based on the UPLC QToF/MS analysis, it is confirmed that the root extract of Rhodomyrtus tomentosa contains flavonoids, tannins, xanthenes, phenols, glycosides, pheromones, chromones, triterpenes and pyranes. Ethyl acetate extract has been found to have good antibacterial activity with zone of inhibition ranging from 9.33 ± 0.21 mm to 13.67 ± 0.32 mm. Antimicrobial activity assay of ethyl acetate extract loaded PVA nanofiber was examined and showed good antibacterial activity. All the four solvent extracts of the plant material and the extract loaded PVA nanofiber have shown not to have any antifungal activity. Rhodomyrtone, known as a natural antibiotic, must contribute to the antibiotic activity against the above bacterial strains. Characterization of nanofibers indicates all weight ratios of the extract loaded PVA slution and the 10% PVA solution has made a nanofiber with a diameter in a range of 76.3 nm and 388.6 nm, respectively and the extracts were well incorporated with in the fibers. 2018-05 Thesis http://umpir.ump.edu.my/id/eprint/24714/ http://umpir.ump.edu.my/id/eprint/24714/1/Antimicrobial%20activities%20of%20the%20crude%20extracts%20and%20electrospun%20nanofibers%20containing%20rhodomyrtus%20tomentosa%20%28kemunting%29%20roots.wm.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Industrial Sciences and Technology Abd Hamid, Hazrulrizawati
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
advisor	Abd Hamid, Hazrulrizawati
topic	RV Botanic Thomsonian and eclectic medicine
spellingShingle	RV Botanic Thomsonian and eclectic medicine Zeyohanness, Senait Sileshi Antimicrobial activities of the crude extracts and electrospun nanofibers containing rhodomyrtus tomentosa (kemunting) roots
description	Traditional medicinal plants can be used to treat chronic and infectious diseases. As a result, the practice of incorporating medicinal plant extracts into polymeric solutions to produce bioactive electrospun nanofibers is rising progressively. In this study Rhodomyrtus tomentosa roots were extracted with hexane, chloroform, ethyl acetate and methanol. Preliminary phytochemical analysis was done by using ultrahighperformance liquid chromatographyquadrupole time-off-light/mass spectrometry (UPLC QToF/MS) method and antimicrobial activity was evaluated against common human pathogenic microorganisms, two Gramnegative bacterial strains (Escherichia coli, Pseudomonas aeruginosa), two Grampositive bacterial strains (Bacillus subtilis, Enterococcus faecalis.) and one fungal strain (Aspergillus niger) by using disk diffusion method and zone of inhibition was determined. The extract with better activity, ethyl acetate extract (EAE), was incorporated with 10% Polyvinyl alcohol (PVA) (W/V) solution at different concentrations (0.25, 0.5, 1.5 and 2.5% W/V) and Ethyl acetate extract of Rhodomyrtus tomentosa roots incorporated in 10% PVA (RTE/PVA) nanofiber were fabricated using electrospinning process. Morphology of the extract loaded nanofibers were characterized using FESEM, FTIR and XRD spectrums. Antimicrobial activity of the electrospun nanofibers was evaluated using same procedures followed during the analysis of all extracts. Based on the UPLC QToF/MS analysis, it is confirmed that the root extract of Rhodomyrtus tomentosa contains flavonoids, tannins, xanthenes, phenols, glycosides, pheromones, chromones, triterpenes and pyranes. Ethyl acetate extract has been found to have good antibacterial activity with zone of inhibition ranging from 9.33 ± 0.21 mm to 13.67 ± 0.32 mm. Antimicrobial activity assay of ethyl acetate extract loaded PVA nanofiber was examined and showed good antibacterial activity. All the four solvent extracts of the plant material and the extract loaded PVA nanofiber have shown not to have any antifungal activity. Rhodomyrtone, known as a natural antibiotic, must contribute to the antibiotic activity against the above bacterial strains. Characterization of nanofibers indicates all weight ratios of the extract loaded PVA slution and the 10% PVA solution has made a nanofiber with a diameter in a range of 76.3 nm and 388.6 nm, respectively and the extracts were well incorporated with in the fibers.
format	Thesis
qualification_level	Master's degree
author	Zeyohanness, Senait Sileshi
author_facet	Zeyohanness, Senait Sileshi
author_sort	Zeyohanness, Senait Sileshi
title	Antimicrobial activities of the crude extracts and electrospun nanofibers containing rhodomyrtus tomentosa (kemunting) roots
title_short	Antimicrobial activities of the crude extracts and electrospun nanofibers containing rhodomyrtus tomentosa (kemunting) roots
title_full	Antimicrobial activities of the crude extracts and electrospun nanofibers containing rhodomyrtus tomentosa (kemunting) roots
title_fullStr	Antimicrobial activities of the crude extracts and electrospun nanofibers containing rhodomyrtus tomentosa (kemunting) roots
title_full_unstemmed	Antimicrobial activities of the crude extracts and electrospun nanofibers containing rhodomyrtus tomentosa (kemunting) roots
title_sort	antimicrobial activities of the crude extracts and electrospun nanofibers containing rhodomyrtus tomentosa (kemunting) roots
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Industrial Sciences and Technology
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/24714/1/Antimicrobial%20activities%20of%20the%20crude%20extracts%20and%20electrospun%20nanofibers%20containing%20rhodomyrtus%20tomentosa%20%28kemunting%29%20roots.wm.pdf
_version_	1783732078340210688

Antimicrobial activities of the crude extracts and electrospun nanofibers containing rhodomyrtus tomentosa (kemunting) roots

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