Study on antioxidant and antidiabetic properties of phloroglucinol from brown macroalgae Padina australis Hauck

Diabetes is a metabolic disorder characterized by high levels of blood glucose. It is caused by the pancreatic insufficiency or by insulin resistance. Marine macroalgae extracts have been established to have strong antidiabetic and antioxidant properties. In the present study, the antidiabetic and a...

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Main Author:	Murugan, Amarchand Chordia
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Q Science (General) T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/25515/1/Study%20on%20antioxidant%20and%20antidiabetic%20properties%20of%20phloroglucinol.pdf
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spelling	my-ump-ir.255152021-08-12T05:13:12Z Study on antioxidant and antidiabetic properties of phloroglucinol from brown macroalgae Padina australis Hauck 2018-08 Murugan, Amarchand Chordia Q Science (General) T Technology (General) Diabetes is a metabolic disorder characterized by high levels of blood glucose. It is caused by the pancreatic insufficiency or by insulin resistance. Marine macroalgae extracts have been established to have strong antidiabetic and antioxidant properties. In the present study, the antidiabetic and antioxidant potential of polyphenolic extract from a brown seaweed Padina australis was evaluated. Methanolic (80:20 % of MeOH: Deionized Water v/v) extract of P. australis was partitioned with five different solvents. The carbolytic enzyme inhibiting functions, radical scavenging activity assay against 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), ferrous ion chelating (FIC) activity, beta-carotene bleaching properties are evaluated. The antiradical and antidiabetic inhibition assays were statistically significant as the different extracts of P. australis inhibited the enzymes in vitro and in vivo. Among the five different fractions, n-butanol fraction showed significantly higher antiradical and antidiabetic inhibitory activity. The methanolic extract was subjected to TLC and HPLC analysis to identify the bioactive molecules present in the mixture of extract. Further LC-MS analysis of n-butanol 5th fraction revealed the presence of three Phlorotannin compounds namely Phloroglucinol, Eckol, and Phlorofucofuroeckol A. Sephadex LH-20, column chromatography technique, was employed to isolate Phloroglucinol compound for further study. Phloroglucinol isolated and purified from the active fraction was evaluated for antidiabetic effect in Streptozotocin-induced diabetic rats. Among the tested portions, n-butanol fraction had significant α- glucosidase and α-amylase inhibitory activity with IC50 value 2.06 ± 0.14 and 2.90 ± 0.08 mg/mL respectively, as compared to Acarbose. Acute toxicity of the isolated pure compound at 20 and 40 mg/kg doses in Swiss Albino mice was found to have potent antidiabetic activity by ameliorating glucose, lipids, and antioxidant parameters. The toxicology studies also showed that the pure compound Phloroglucinol did not have any toxic effects in the internal organs; biochemical and hematological parameters. These findings suggest that the dietary use of Padina australis and the isolated compound Phloroglucinol can be used as medicine for the treatment of diabetes. This work could help to develop new oral antihyperglycemic agent in the management of diabetes mellitus. 2018-08 Thesis http://umpir.ump.edu.my/id/eprint/25515/ http://umpir.ump.edu.my/id/eprint/25515/1/Study%20on%20antioxidant%20and%20antidiabetic%20properties%20of%20phloroglucinol.pdf pdf en public phd doctoral Universiti Malaysia Pahang Faculty of Industrial Sciences and Technology
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	Q Science (General) T Technology (General)
spellingShingle	Q Science (General) T Technology (General) Murugan, Amarchand Chordia Study on antioxidant and antidiabetic properties of phloroglucinol from brown macroalgae Padina australis Hauck
description	Diabetes is a metabolic disorder characterized by high levels of blood glucose. It is caused by the pancreatic insufficiency or by insulin resistance. Marine macroalgae extracts have been established to have strong antidiabetic and antioxidant properties. In the present study, the antidiabetic and antioxidant potential of polyphenolic extract from a brown seaweed Padina australis was evaluated. Methanolic (80:20 % of MeOH: Deionized Water v/v) extract of P. australis was partitioned with five different solvents. The carbolytic enzyme inhibiting functions, radical scavenging activity assay against 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), ferrous ion chelating (FIC) activity, beta-carotene bleaching properties are evaluated. The antiradical and antidiabetic inhibition assays were statistically significant as the different extracts of P. australis inhibited the enzymes in vitro and in vivo. Among the five different fractions, n-butanol fraction showed significantly higher antiradical and antidiabetic inhibitory activity. The methanolic extract was subjected to TLC and HPLC analysis to identify the bioactive molecules present in the mixture of extract. Further LC-MS analysis of n-butanol 5th fraction revealed the presence of three Phlorotannin compounds namely Phloroglucinol, Eckol, and Phlorofucofuroeckol A. Sephadex LH-20, column chromatography technique, was employed to isolate Phloroglucinol compound for further study. Phloroglucinol isolated and purified from the active fraction was evaluated for antidiabetic effect in Streptozotocin-induced diabetic rats. Among the tested portions, n-butanol fraction had significant α- glucosidase and α-amylase inhibitory activity with IC50 value 2.06 ± 0.14 and 2.90 ± 0.08 mg/mL respectively, as compared to Acarbose. Acute toxicity of the isolated pure compound at 20 and 40 mg/kg doses in Swiss Albino mice was found to have potent antidiabetic activity by ameliorating glucose, lipids, and antioxidant parameters. The toxicology studies also showed that the pure compound Phloroglucinol did not have any toxic effects in the internal organs; biochemical and hematological parameters. These findings suggest that the dietary use of Padina australis and the isolated compound Phloroglucinol can be used as medicine for the treatment of diabetes. This work could help to develop new oral antihyperglycemic agent in the management of diabetes mellitus.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Murugan, Amarchand Chordia
author_facet	Murugan, Amarchand Chordia
author_sort	Murugan, Amarchand Chordia
title	Study on antioxidant and antidiabetic properties of phloroglucinol from brown macroalgae Padina australis Hauck
title_short	Study on antioxidant and antidiabetic properties of phloroglucinol from brown macroalgae Padina australis Hauck
title_full	Study on antioxidant and antidiabetic properties of phloroglucinol from brown macroalgae Padina australis Hauck
title_fullStr	Study on antioxidant and antidiabetic properties of phloroglucinol from brown macroalgae Padina australis Hauck
title_full_unstemmed	Study on antioxidant and antidiabetic properties of phloroglucinol from brown macroalgae Padina australis Hauck
title_sort	study on antioxidant and antidiabetic properties of phloroglucinol from brown macroalgae padina australis hauck
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Industrial Sciences and Technology
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/25515/1/Study%20on%20antioxidant%20and%20antidiabetic%20properties%20of%20phloroglucinol.pdf
_version_	1783732096091553792

Study on antioxidant and antidiabetic properties of phloroglucinol from brown macroalgae Padina australis Hauck

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