Microwave assisted extraction (MAE) of beta-sitosterol from plant legumes pod

Archidendron pauciflorum or Jering, Petai or Parkia speciosa, and Leucaena leucocephala or Petai Belalang are all known as a local delicacy to many countries in Asia and yet also have the beneficial aspect in health such as an antimicrobial, antioxidant, hypoglycaemic, antiulcer and anti-hypertensio...

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Main Author:	Norlaili, Hashim
Format:	Thesis
Language:	English
Published:	2019
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/29024/1/Microwave%20assisted%20extraction%20%28MAE%29%20of%20beta-sitosterol%20from%20plant%20legumes%20pod.wm.pdf
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spelling	my-ump-ir.290242023-05-17T03:22:57Z Microwave assisted extraction (MAE) of beta-sitosterol from plant legumes pod 2019-03 Norlaili, Hashim TP Chemical technology Archidendron pauciflorum or Jering, Petai or Parkia speciosa, and Leucaena leucocephala or Petai Belalang are all known as a local delicacy to many countries in Asia and yet also have the beneficial aspect in health such as an antimicrobial, antioxidant, hypoglycaemic, antiulcer and anti-hypertension. Present studies only shows on the present of phytosteroid in the plant but not its concentration. The extraction process of beta-sitosterol performed by using Microwave-Assisted Extraction process. After the extraction process, the characterization process was done by using ultraviolet-visible spectroscopy (UV-VIS), Fourier transform infrared (FTIR), high-performance liquid chromatographic analysis (HPLC) and scanning electron microscope (SEM). UV-Vis analysis analyse concentration of beta-sitosterol content in the legume pod while the FTIR will give the functional group of the legume pod. SEM shows the condition of plant surface before and after the extraction process and to ensure the extraction process at desired parameter condition. In order to optimize the maximum yield extraction of beta-sitosterol, the extraction parameter was optimize by using response surface method (RSM) by using Design Expert 7.0. The optimization process was done by using one factor at a time (OFAT) for the screening process where the research will be continued with legume pod that content with the highest yield of beta-sitosterol, fractional factorial design (FFD) for screening extraction process parameter and central composite design (CCD) for the optimization of the extraction process will be required in order to plan and investigate the most required processes. OFAT process was done by using four parameters, which are extraction duration, irradiation power, solvent concentration and extraction temperature with all three types of plant. From OFAT study result, FFD was continued with time and solvent concentration with only L. leucocephala legume. CCD result was optimized with 62.5% ethanol solvent, 7.45 min of extraction duration, 600W irradiation power and 75ºC irradiation temperature to give the highest yield of beta-sitosterol which is 0.31 mg/mL. 2019-03 Thesis http://umpir.ump.edu.my/id/eprint/29024/ http://umpir.ump.edu.my/id/eprint/29024/1/Microwave%20assisted%20extraction%20%28MAE%29%20of%20beta-sitosterol%20from%20plant%20legumes%20pod.wm.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Chemical & Natural Resources Engineering Noormazlinah, Ahmad
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
advisor	Noormazlinah, Ahmad
topic	TP Chemical technology
spellingShingle	TP Chemical technology Norlaili, Hashim Microwave assisted extraction (MAE) of beta-sitosterol from plant legumes pod
description	Archidendron pauciflorum or Jering, Petai or Parkia speciosa, and Leucaena leucocephala or Petai Belalang are all known as a local delicacy to many countries in Asia and yet also have the beneficial aspect in health such as an antimicrobial, antioxidant, hypoglycaemic, antiulcer and anti-hypertension. Present studies only shows on the present of phytosteroid in the plant but not its concentration. The extraction process of beta-sitosterol performed by using Microwave-Assisted Extraction process. After the extraction process, the characterization process was done by using ultraviolet-visible spectroscopy (UV-VIS), Fourier transform infrared (FTIR), high-performance liquid chromatographic analysis (HPLC) and scanning electron microscope (SEM). UV-Vis analysis analyse concentration of beta-sitosterol content in the legume pod while the FTIR will give the functional group of the legume pod. SEM shows the condition of plant surface before and after the extraction process and to ensure the extraction process at desired parameter condition. In order to optimize the maximum yield extraction of beta-sitosterol, the extraction parameter was optimize by using response surface method (RSM) by using Design Expert 7.0. The optimization process was done by using one factor at a time (OFAT) for the screening process where the research will be continued with legume pod that content with the highest yield of beta-sitosterol, fractional factorial design (FFD) for screening extraction process parameter and central composite design (CCD) for the optimization of the extraction process will be required in order to plan and investigate the most required processes. OFAT process was done by using four parameters, which are extraction duration, irradiation power, solvent concentration and extraction temperature with all three types of plant. From OFAT study result, FFD was continued with time and solvent concentration with only L. leucocephala legume. CCD result was optimized with 62.5% ethanol solvent, 7.45 min of extraction duration, 600W irradiation power and 75ºC irradiation temperature to give the highest yield of beta-sitosterol which is 0.31 mg/mL.
format	Thesis
qualification_level	Master's degree
author	Norlaili, Hashim
author_facet	Norlaili, Hashim
author_sort	Norlaili, Hashim
title	Microwave assisted extraction (MAE) of beta-sitosterol from plant legumes pod
title_short	Microwave assisted extraction (MAE) of beta-sitosterol from plant legumes pod
title_full	Microwave assisted extraction (MAE) of beta-sitosterol from plant legumes pod
title_fullStr	Microwave assisted extraction (MAE) of beta-sitosterol from plant legumes pod
title_full_unstemmed	Microwave assisted extraction (MAE) of beta-sitosterol from plant legumes pod
title_sort	microwave assisted extraction (mae) of beta-sitosterol from plant legumes pod
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Chemical & Natural Resources Engineering
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/29024/1/Microwave%20assisted%20extraction%20%28MAE%29%20of%20beta-sitosterol%20from%20plant%20legumes%20pod.wm.pdf
_version_	1783732114417516544

Microwave assisted extraction (MAE) of beta-sitosterol from plant legumes pod

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