Study on antiproliferative activity of hypsizygus tessellatus and flammulina velutipes extracts adsorbed on sulphated zirconia nanoparticles against breast cancer cell lines

In this study, the in vitro biological activities of water, methanol, acetone, and ethyl acetate extracts of two Hypsizygus tessellatus variants (brown Buna shimeji and white Bunapi shimeji) and Flammulina velutipes (Enoki) were investigated. The caps and stems of the mushrooms were extracted with d...

Full description

Saved in:

Bibliographic Details
Main Author:	Ishmael, Ukaegbu Chinonso
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Q Science (General) T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/29282/1/Study%20on%20antiproliferative%20activity%20of%20hypsizygus%20tessellatus%20and%20flammulina%20velutipes.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-ump-ir.29282
record_format	uketd_dc
spelling	my-ump-ir.292822020-09-10T07:54:21Z Study on antiproliferative activity of hypsizygus tessellatus and flammulina velutipes extracts adsorbed on sulphated zirconia nanoparticles against breast cancer cell lines 2019-05 Ishmael, Ukaegbu Chinonso Q Science (General) RV Botanic, Thomsonian, and eclectic medicine T Technology (General) In this study, the in vitro biological activities of water, methanol, acetone, and ethyl acetate extracts of two Hypsizygus tessellatus variants (brown Buna shimeji and white Bunapi shimeji) and Flammulina velutipes (Enoki) were investigated. The caps and stems of the mushrooms were extracted with different solvents and chemically characterized for total phenolic content (TPC), total flavonoids content (TFC), antioxidant activities (against DPPH and FRAP). Furthermore, the extracted phytochemicals from the mushrooms were identified using Ultra performance liquid chromatography quadrupole time of flight mass spectrometer (UPLC-QTOF/MS). A multiple correspondence analysis (MCA) was performed on the extracted phytochemicals from the mushrooms based on the solvents used during the extraction process. Then, the antiproliferative activity of the potent fractions were evaluated against two breast cancer cell lines (MCF-7 and MDA-MB-231) using MTT assay. Further investigations were carried out on the antiproliferative activity of the mushroom extracts adsorbed on sulphated zirconia nanoparticles (SZN). The results of this study showed the mushroom caps extracts to have more TPC and TFC compared to the stem extracts. Enoki cap extracts contained higher TPC and TFC compared to Buna shimeji and Bunapi shimeji cap extracts. The mushroom order of the observed antioxidant activity of the extracts was: Enoki > Bunapi shimeji > Buna shimeji, and in the solvent order: water > methanol > acetone > ethyl acetate. The TPC of the extracts showed a positive correlation with their antioxidant activities whole the MCA analysis showed a positive correlation between the extraction solvents and the extracted phytochemicals from the mushrooms. The antiproliferative study of the extracts using two cancer cell lines (MCF-7 and MDA-MB-231) showed a considerable level of sensitivity of the cell lines to water and methanol extracts of Enoki and Bunapi shimeji caps. Among the extracts, Enoki caps water extract showed the highest antiproliferative activity against MCF-7 (IC50 value ranged from 14.42–24.84 μg/mL) and MDA-MB-231 (IC50 value ranged from 151.57 – 227.99 μg/mL) after 72 h. SZN also showed a considerable level of antiproliferative activity against the cancer cell lines at the studied concentrations while the Normal (normal) cells were less sensitive to SZN (IC50 value range of 130.7 – 134.1 μg/mL) compared to MCF-7 cells (IC50 value range of 36.5 – 37.0 μg/mL) and MDA-MB-231 cells (IC50 value range of 68.9 – 70.9 μg/mL). The adsorption of the extracts on SZN synergistically improved their antiproliferative activities against the cancer cell lines (p < 0.05), but Enoki caps water extract showed the best antiproliferative activity after adsorption on SZN. Conclusively, water and methanol extracts of Enoki and Bunapi shimeji caps significantly inhibited the in vitro proliferation of MCF-7 and MDA-MB 231, and it can be suggested that their antiproliferative activity against the cancer cell lines was enhanced through adsorption of the phytochemicals on the sulphated zirconia nanoparticles. 2019-05 Thesis http://umpir.ump.edu.my/id/eprint/29282/ http://umpir.ump.edu.my/id/eprint/29282/1/Study%20on%20antiproliferative%20activity%20of%20hypsizygus%20tessellatus%20and%20flammulina%20velutipes.pdf pdf en public phd doctoral Universiti Malaysia Pahang Faculty of Industrial Sciences and Technology
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	Q Science (General) Q Science (General) T Technology (General)
spellingShingle	Q Science (General) Q Science (General) T Technology (General) Ishmael, Ukaegbu Chinonso Study on antiproliferative activity of hypsizygus tessellatus and flammulina velutipes extracts adsorbed on sulphated zirconia nanoparticles against breast cancer cell lines
description	In this study, the in vitro biological activities of water, methanol, acetone, and ethyl acetate extracts of two Hypsizygus tessellatus variants (brown Buna shimeji and white Bunapi shimeji) and Flammulina velutipes (Enoki) were investigated. The caps and stems of the mushrooms were extracted with different solvents and chemically characterized for total phenolic content (TPC), total flavonoids content (TFC), antioxidant activities (against DPPH and FRAP). Furthermore, the extracted phytochemicals from the mushrooms were identified using Ultra performance liquid chromatography quadrupole time of flight mass spectrometer (UPLC-QTOF/MS). A multiple correspondence analysis (MCA) was performed on the extracted phytochemicals from the mushrooms based on the solvents used during the extraction process. Then, the antiproliferative activity of the potent fractions were evaluated against two breast cancer cell lines (MCF-7 and MDA-MB-231) using MTT assay. Further investigations were carried out on the antiproliferative activity of the mushroom extracts adsorbed on sulphated zirconia nanoparticles (SZN). The results of this study showed the mushroom caps extracts to have more TPC and TFC compared to the stem extracts. Enoki cap extracts contained higher TPC and TFC compared to Buna shimeji and Bunapi shimeji cap extracts. The mushroom order of the observed antioxidant activity of the extracts was: Enoki > Bunapi shimeji > Buna shimeji, and in the solvent order: water > methanol > acetone > ethyl acetate. The TPC of the extracts showed a positive correlation with their antioxidant activities whole the MCA analysis showed a positive correlation between the extraction solvents and the extracted phytochemicals from the mushrooms. The antiproliferative study of the extracts using two cancer cell lines (MCF-7 and MDA-MB-231) showed a considerable level of sensitivity of the cell lines to water and methanol extracts of Enoki and Bunapi shimeji caps. Among the extracts, Enoki caps water extract showed the highest antiproliferative activity against MCF-7 (IC50 value ranged from 14.42–24.84 μg/mL) and MDA-MB-231 (IC50 value ranged from 151.57 – 227.99 μg/mL) after 72 h. SZN also showed a considerable level of antiproliferative activity against the cancer cell lines at the studied concentrations while the Normal (normal) cells were less sensitive to SZN (IC50 value range of 130.7 – 134.1 μg/mL) compared to MCF-7 cells (IC50 value range of 36.5 – 37.0 μg/mL) and MDA-MB-231 cells (IC50 value range of 68.9 – 70.9 μg/mL). The adsorption of the extracts on SZN synergistically improved their antiproliferative activities against the cancer cell lines (p < 0.05), but Enoki caps water extract showed the best antiproliferative activity after adsorption on SZN. Conclusively, water and methanol extracts of Enoki and Bunapi shimeji caps significantly inhibited the in vitro proliferation of MCF-7 and MDA-MB 231, and it can be suggested that their antiproliferative activity against the cancer cell lines was enhanced through adsorption of the phytochemicals on the sulphated zirconia nanoparticles.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ishmael, Ukaegbu Chinonso
author_facet	Ishmael, Ukaegbu Chinonso
author_sort	Ishmael, Ukaegbu Chinonso
title	Study on antiproliferative activity of hypsizygus tessellatus and flammulina velutipes extracts adsorbed on sulphated zirconia nanoparticles against breast cancer cell lines
title_short	Study on antiproliferative activity of hypsizygus tessellatus and flammulina velutipes extracts adsorbed on sulphated zirconia nanoparticles against breast cancer cell lines
title_full	Study on antiproliferative activity of hypsizygus tessellatus and flammulina velutipes extracts adsorbed on sulphated zirconia nanoparticles against breast cancer cell lines
title_fullStr	Study on antiproliferative activity of hypsizygus tessellatus and flammulina velutipes extracts adsorbed on sulphated zirconia nanoparticles against breast cancer cell lines
title_full_unstemmed	Study on antiproliferative activity of hypsizygus tessellatus and flammulina velutipes extracts adsorbed on sulphated zirconia nanoparticles against breast cancer cell lines
title_sort	study on antiproliferative activity of hypsizygus tessellatus and flammulina velutipes extracts adsorbed on sulphated zirconia nanoparticles against breast cancer cell lines
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Industrial Sciences and Technology
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/29282/1/Study%20on%20antiproliferative%20activity%20of%20hypsizygus%20tessellatus%20and%20flammulina%20velutipes.pdf
_version_	1783732121289883648

Study on antiproliferative activity of hypsizygus tessellatus and flammulina velutipes extracts adsorbed on sulphated zirconia nanoparticles against breast cancer cell lines

Similar Items