Study on the extraction of high methoxyl pectin from dragon fruit (hylocereus polyrhizus) peels and its adsorption performance againts cholesterol

Pectin is a high value soluble dietary fiber which is beneficial to health. Requirement to manufacture the high yield of pectin with certain characteristic is a major challenge. Pectin characteristic is the keywords to its application. The purpose of this study is to investigate the extraction of hy...

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Main Author:	Rubaiyi, Mat Zaid
Format:	Thesis
Language:	English
Published:	2019
Subjects:	TA Engineering (General) Civil engineering (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/31081/1/Study%20on%20the%20extraction%20of%20high%20methoxyl%20pectin%20from%20dragon%20fruit%20%28hylocereus%20polyrhizus%29.pdf
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spelling	my-ump-ir.310812021-04-08T02:07:31Z Study on the extraction of high methoxyl pectin from dragon fruit (hylocereus polyrhizus) peels and its adsorption performance againts cholesterol 2019-10 Rubaiyi, Mat Zaid TA Engineering (General). Civil engineering (General) Pectin is a high value soluble dietary fiber which is beneficial to health. Requirement to manufacture the high yield of pectin with certain characteristic is a major challenge. Pectin characteristic is the keywords to its application. The purpose of this study is to investigate the extraction of hydrophobic polysaccharide which is known as high methoxyl pectin extracted from Hylocereus polyrhizus peels as potential hypolipidemic agents by in vitro sorption experiments on model molecules (cholesterol). In this study, combination of physical and chemical treatment has been applied in the extraction process. Mild ultrasonic by sonicator bath and agitation by propeller with citric acid as an extraction solvent was employed in the extraction process to achieve the high yield of high methoxyl pectin. A study on the extraction parameters (agitation rate, temperature, time, pH and liquid-solid ratio (LSR)) was performed by one-factor-at-a-time (OFAT) to facilitate the appropriate range for further optimization process. The extraction process kinetics has been done after OFAT study which would give the idea for process forecasting and optimization of the pectin yield. The kinetic analysis by Panchev’s model shows that the extraction rate was found highest at LSR 10 v/w with ymax 30.85 %. The calculated activation energy for pectin dissolution and degradation was found to be 4.532 kJ/mol and 28.054 kJ/mol, respectively. The initial screening using 25 full factorial design indicated that temperature, time, pH and LSR were significant factors in the extraction of high yield of high methoxyl pectin. All the significant factors were then optimized using response surface methodology (RSM) to achieve the high yield of high methoxyl pectin. The value of pectin yield and degree of esterification (DE) after optimization were 30.11 % and 55.00 %, respectively as compared to the initial pectin yield and DE of 21.52 % and 56.1 %, respectively. High methoxyl pectin extracted from Hylocereus polyrhizus peels (HMPHPP) were tested as potential cholesterol-lowering agent and compared with the commercial high methoxyl citrus pectin (HMPCP). The cholesterol sorption was measured quantitatively by high performance liquid chromatography (HPLC). It was found that high methoxyl pectin from Hylocereus polyrhizus peels has the affinity towards cholesterol which followed the Freundlich isotherm and pseudo-first order model. Hydrophobicity and surface structure of the HPP was characterized by contact angle analysis and field emission scanning electron microscopy (FESEM) in order understand HMPHPP surface that help the adsorption process. This study proves that high methoxyl pectin extracted from Hylocereus polyrhizus peels has affinity towards cholesterol which help the adsorption of cholesterol. 2019-10 Thesis http://umpir.ump.edu.my/id/eprint/31081/ http://umpir.ump.edu.my/id/eprint/31081/1/Study%20on%20the%20extraction%20of%20high%20methoxyl%20pectin%20from%20dragon%20fruit%20%28hylocereus%20polyrhizus%29.pdf pdf en public phd doctoral Universiti Malaysia Pahang Faculty of Civil Engineering Technology
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	TA Engineering (General) Civil engineering (General)
spellingShingle	TA Engineering (General) Civil engineering (General) Rubaiyi, Mat Zaid Study on the extraction of high methoxyl pectin from dragon fruit (hylocereus polyrhizus) peels and its adsorption performance againts cholesterol
description	Pectin is a high value soluble dietary fiber which is beneficial to health. Requirement to manufacture the high yield of pectin with certain characteristic is a major challenge. Pectin characteristic is the keywords to its application. The purpose of this study is to investigate the extraction of hydrophobic polysaccharide which is known as high methoxyl pectin extracted from Hylocereus polyrhizus peels as potential hypolipidemic agents by in vitro sorption experiments on model molecules (cholesterol). In this study, combination of physical and chemical treatment has been applied in the extraction process. Mild ultrasonic by sonicator bath and agitation by propeller with citric acid as an extraction solvent was employed in the extraction process to achieve the high yield of high methoxyl pectin. A study on the extraction parameters (agitation rate, temperature, time, pH and liquid-solid ratio (LSR)) was performed by one-factor-at-a-time (OFAT) to facilitate the appropriate range for further optimization process. The extraction process kinetics has been done after OFAT study which would give the idea for process forecasting and optimization of the pectin yield. The kinetic analysis by Panchev’s model shows that the extraction rate was found highest at LSR 10 v/w with ymax 30.85 %. The calculated activation energy for pectin dissolution and degradation was found to be 4.532 kJ/mol and 28.054 kJ/mol, respectively. The initial screening using 25 full factorial design indicated that temperature, time, pH and LSR were significant factors in the extraction of high yield of high methoxyl pectin. All the significant factors were then optimized using response surface methodology (RSM) to achieve the high yield of high methoxyl pectin. The value of pectin yield and degree of esterification (DE) after optimization were 30.11 % and 55.00 %, respectively as compared to the initial pectin yield and DE of 21.52 % and 56.1 %, respectively. High methoxyl pectin extracted from Hylocereus polyrhizus peels (HMPHPP) were tested as potential cholesterol-lowering agent and compared with the commercial high methoxyl citrus pectin (HMPCP). The cholesterol sorption was measured quantitatively by high performance liquid chromatography (HPLC). It was found that high methoxyl pectin from Hylocereus polyrhizus peels has the affinity towards cholesterol which followed the Freundlich isotherm and pseudo-first order model. Hydrophobicity and surface structure of the HPP was characterized by contact angle analysis and field emission scanning electron microscopy (FESEM) in order understand HMPHPP surface that help the adsorption process. This study proves that high methoxyl pectin extracted from Hylocereus polyrhizus peels has affinity towards cholesterol which help the adsorption of cholesterol.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Rubaiyi, Mat Zaid
author_facet	Rubaiyi, Mat Zaid
author_sort	Rubaiyi, Mat Zaid
title	Study on the extraction of high methoxyl pectin from dragon fruit (hylocereus polyrhizus) peels and its adsorption performance againts cholesterol
title_short	Study on the extraction of high methoxyl pectin from dragon fruit (hylocereus polyrhizus) peels and its adsorption performance againts cholesterol
title_full	Study on the extraction of high methoxyl pectin from dragon fruit (hylocereus polyrhizus) peels and its adsorption performance againts cholesterol
title_fullStr	Study on the extraction of high methoxyl pectin from dragon fruit (hylocereus polyrhizus) peels and its adsorption performance againts cholesterol
title_full_unstemmed	Study on the extraction of high methoxyl pectin from dragon fruit (hylocereus polyrhizus) peels and its adsorption performance againts cholesterol
title_sort	study on the extraction of high methoxyl pectin from dragon fruit (hylocereus polyrhizus) peels and its adsorption performance againts cholesterol
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Civil Engineering Technology
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/31081/1/Study%20on%20the%20extraction%20of%20high%20methoxyl%20pectin%20from%20dragon%20fruit%20%28hylocereus%20polyrhizus%29.pdf
_version_	1783732150884892672

Study on the extraction of high methoxyl pectin from dragon fruit (hylocereus polyrhizus) peels and its adsorption performance againts cholesterol

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