Solid state fermentation of Monascus purpureus in stirred drum bioreactor for production of red pigment from oil palm frond

Various concern have been reported regarding the effect of existing synthetic colorant, hence, mass production of natural colorant seems to be a potential alternative in fulfilling the demand of colorant in industries. This research has emphasized on the process capability of stirred drum bioreactor...

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Main Author:	Mohamad Al Aamin, Razali
Format:	Thesis
Language:	English
Published:	2019
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/31092/1/Solid%20state%20fermentation%20of%20monascus%20purpureus%20in%20stirred%20drum%20bioreactor%20for%20production%20of%20red%20pigment.pdf
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spelling	my-ump-ir.310922021-04-08T09:02:30Z Solid state fermentation of Monascus purpureus in stirred drum bioreactor for production of red pigment from oil palm frond 2019-09 Mohamad Al Aamin, Razali TP Chemical technology Various concern have been reported regarding the effect of existing synthetic colorant, hence, mass production of natural colorant seems to be a potential alternative in fulfilling the demand of colorant in industries. This research has emphasized on the process capability of stirred drum bioreactor on performing solid state fermentation (SSF) of Monascus purpureus FTC 5357 by using oil palm frond (OPF) for production of red pigment. Seven parameters, namely, initial moisture content (% v/w), inoculum size (spores/mL), peptone concentration (% w/w), initial pH, aeration rate (vvm), loading capacity (% v/v) and agitation programme (cycles/day) were evaluated for red pigment production, biomass, and glucose consumption. One Factor at A Time (OFAT) and two level factorial screening designs were used in the study. Three responses, which were red pigment production, biomass, and glucose consumption have been used as responses to identify the significant factors affecting the SSF process. In OFAT studies, the highest pigment production was recorded at aeration rate of 1 vvm (6.09 AU/g) and the lowest production was obtained at 45% of initial moisture content (0.1 AU/g). In two level factorial design experimental studies, the highest pigment production was 71.86 AU/g by incorporating 75% v/w of initial moisture content, 108 spores/mL of inoculum size, initial pH of 4, aeration rate of 1.21 vvm, loading capacity of 35 % v/v and agitation programme of 6 cycles/day. The most significant factor contributing to the red pigment production was initial moisture content, with F-value and p-value of 46.54 and 0.0002, respectively, followed by inoculum size, with F-value of 14.12 and p-value of 0.0071, respectively. Similar observation also occurred for biomass, shows that highest contribution from initial moisture content with F-value of 55.07 and p-value of 0.0051, followed by peptone with F-value of 20.47 and p-value of 0.0202, respectively. In conclusion, this work demonstrated the potential of using stirred drum bioreactor and OPF substrate in producing higher yield of red pigment production (71.86 AU/g) compared to shake flask fermentation (2.3 AU/g). 2019-09 Thesis http://umpir.ump.edu.my/id/eprint/31092/ http://umpir.ump.edu.my/id/eprint/31092/1/Solid%20state%20fermentation%20of%20monascus%20purpureus%20in%20stirred%20drum%20bioreactor%20for%20production%20of%20red%20pigment.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Chemical & Natural Resources Engineering
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Mohamad Al Aamin, Razali Solid state fermentation of Monascus purpureus in stirred drum bioreactor for production of red pigment from oil palm frond
description	Various concern have been reported regarding the effect of existing synthetic colorant, hence, mass production of natural colorant seems to be a potential alternative in fulfilling the demand of colorant in industries. This research has emphasized on the process capability of stirred drum bioreactor on performing solid state fermentation (SSF) of Monascus purpureus FTC 5357 by using oil palm frond (OPF) for production of red pigment. Seven parameters, namely, initial moisture content (% v/w), inoculum size (spores/mL), peptone concentration (% w/w), initial pH, aeration rate (vvm), loading capacity (% v/v) and agitation programme (cycles/day) were evaluated for red pigment production, biomass, and glucose consumption. One Factor at A Time (OFAT) and two level factorial screening designs were used in the study. Three responses, which were red pigment production, biomass, and glucose consumption have been used as responses to identify the significant factors affecting the SSF process. In OFAT studies, the highest pigment production was recorded at aeration rate of 1 vvm (6.09 AU/g) and the lowest production was obtained at 45% of initial moisture content (0.1 AU/g). In two level factorial design experimental studies, the highest pigment production was 71.86 AU/g by incorporating 75% v/w of initial moisture content, 108 spores/mL of inoculum size, initial pH of 4, aeration rate of 1.21 vvm, loading capacity of 35 % v/v and agitation programme of 6 cycles/day. The most significant factor contributing to the red pigment production was initial moisture content, with F-value and p-value of 46.54 and 0.0002, respectively, followed by inoculum size, with F-value of 14.12 and p-value of 0.0071, respectively. Similar observation also occurred for biomass, shows that highest contribution from initial moisture content with F-value of 55.07 and p-value of 0.0051, followed by peptone with F-value of 20.47 and p-value of 0.0202, respectively. In conclusion, this work demonstrated the potential of using stirred drum bioreactor and OPF substrate in producing higher yield of red pigment production (71.86 AU/g) compared to shake flask fermentation (2.3 AU/g).
format	Thesis
qualification_level	Master's degree
author	Mohamad Al Aamin, Razali
author_facet	Mohamad Al Aamin, Razali
author_sort	Mohamad Al Aamin, Razali
title	Solid state fermentation of Monascus purpureus in stirred drum bioreactor for production of red pigment from oil palm frond
title_short	Solid state fermentation of Monascus purpureus in stirred drum bioreactor for production of red pigment from oil palm frond
title_full	Solid state fermentation of Monascus purpureus in stirred drum bioreactor for production of red pigment from oil palm frond
title_fullStr	Solid state fermentation of Monascus purpureus in stirred drum bioreactor for production of red pigment from oil palm frond
title_full_unstemmed	Solid state fermentation of Monascus purpureus in stirred drum bioreactor for production of red pigment from oil palm frond
title_sort	solid state fermentation of monascus purpureus in stirred drum bioreactor for production of red pigment from oil palm frond
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Chemical & Natural Resources Engineering
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/31092/1/Solid%20state%20fermentation%20of%20monascus%20purpureus%20in%20stirred%20drum%20bioreactor%20for%20production%20of%20red%20pigment.pdf
_version_	1783732152507039744

Solid state fermentation of Monascus purpureus in stirred drum bioreactor for production of red pigment from oil palm frond

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