Biosynthesis of biobutanol from oil palm frond juice by Clostridium acetobutylicum

Energy from biomass resources is becoming increasingly important, since it can be used to partly displace conventional sources of energy. The Malaysian oil palm industry generates huge quantities of lignocellulosic biomass which created a major disposal problem. Therefore, oil palm frond (OPF) juice...

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Main Author:	Nur Syazana, Muhamad Nasrah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/31112/1/Biosynthesis%20of%20biobutanol%20from%20oil%20palm%20frond%20juice%20by%20clostridium%20acetobutylicum.wm.pdf
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spelling	my-ump-ir.311122023-05-09T03:04:07Z Biosynthesis of biobutanol from oil palm frond juice by Clostridium acetobutylicum 2019-11 Nur Syazana, Muhamad Nasrah TP Chemical technology Energy from biomass resources is becoming increasingly important, since it can be used to partly displace conventional sources of energy. The Malaysian oil palm industry generates huge quantities of lignocellulosic biomass which created a major disposal problem. Therefore, oil palm frond (OPF) juice was introduced as a substrate in Acetone-Butanol-Ethanol (ABE) fermentation to produce biobutanol by Clostridium acetobutylicum ATCC 824. This study aims to investigate the potential of OPF juice as a substrate for the butanol production. During preliminary study, the production in OPF juice was compared with synthetic sugar as control experiment. Next, this study proceeds with the second and third objective to screen and optimize the factors affecting ABE fermentation by C. acetobutylicum ATCC 824. Sugar content in OPF juice was determined to identify the initial sugar concentration for the fermentation. Response Surface Methodology (RSM) was employed to screen and optimize the butanol production. The total sugar analyzed using High Performance Liquid Chromatography (HPLC) in OPF juice was 68.58 g/L, with glucose, sucrose and fructose value 48.19 g/L, 8.48 g/L and 11.91 g/L, respectively. The culture produced 9.24 g/L of biobutanol using OPF juice with 0.24 g/g biobutanol yield. Meanwhile, 10.91 g/L biobutanol produced using synthetic sugars as control experiment with 0.27 g/g biobutanol yield. The biobutanol yield produce by synthetic sugar in control experiment seem comparable to the fermentation in OPF juice, with only 11.25% higher than OPF juice. In factorial analysis, yeast extract concentration was the highest factor affecting the fermentation process with 8.20% contribution. The second and third highest contribution factors was inoculum size and incubation temperature. These three factors were then optimized using RSM. The optimum condition of the fermentation was found out at 10% inoculum size, 37°C incubation temperature and 5.5 g/L yeast extract concentration which 0.2992 g/g biobutanol yield was obtained in validation process. These experimental findings were in close agreement with the model prediction, with a difference only 9.76%. Overall, ABE fermentation to produce biobutanol using OPF juice by C. acetobutylicum ATCC 824 has high potential which later can be used as commercial substrate in biofuel industry. 2019-11 Thesis http://umpir.ump.edu.my/id/eprint/31112/ http://umpir.ump.edu.my/id/eprint/31112/1/Biosynthesis%20of%20biobutanol%20from%20oil%20palm%20frond%20juice%20by%20clostridium%20acetobutylicum.wm.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Chemical and Process Engineering Technology Mohd Zahari, Mior Ahmad Khushairi
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
advisor	Mohd Zahari, Mior Ahmad Khushairi
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nur Syazana, Muhamad Nasrah Biosynthesis of biobutanol from oil palm frond juice by Clostridium acetobutylicum
description	Energy from biomass resources is becoming increasingly important, since it can be used to partly displace conventional sources of energy. The Malaysian oil palm industry generates huge quantities of lignocellulosic biomass which created a major disposal problem. Therefore, oil palm frond (OPF) juice was introduced as a substrate in Acetone-Butanol-Ethanol (ABE) fermentation to produce biobutanol by Clostridium acetobutylicum ATCC 824. This study aims to investigate the potential of OPF juice as a substrate for the butanol production. During preliminary study, the production in OPF juice was compared with synthetic sugar as control experiment. Next, this study proceeds with the second and third objective to screen and optimize the factors affecting ABE fermentation by C. acetobutylicum ATCC 824. Sugar content in OPF juice was determined to identify the initial sugar concentration for the fermentation. Response Surface Methodology (RSM) was employed to screen and optimize the butanol production. The total sugar analyzed using High Performance Liquid Chromatography (HPLC) in OPF juice was 68.58 g/L, with glucose, sucrose and fructose value 48.19 g/L, 8.48 g/L and 11.91 g/L, respectively. The culture produced 9.24 g/L of biobutanol using OPF juice with 0.24 g/g biobutanol yield. Meanwhile, 10.91 g/L biobutanol produced using synthetic sugars as control experiment with 0.27 g/g biobutanol yield. The biobutanol yield produce by synthetic sugar in control experiment seem comparable to the fermentation in OPF juice, with only 11.25% higher than OPF juice. In factorial analysis, yeast extract concentration was the highest factor affecting the fermentation process with 8.20% contribution. The second and third highest contribution factors was inoculum size and incubation temperature. These three factors were then optimized using RSM. The optimum condition of the fermentation was found out at 10% inoculum size, 37°C incubation temperature and 5.5 g/L yeast extract concentration which 0.2992 g/g biobutanol yield was obtained in validation process. These experimental findings were in close agreement with the model prediction, with a difference only 9.76%. Overall, ABE fermentation to produce biobutanol using OPF juice by C. acetobutylicum ATCC 824 has high potential which later can be used as commercial substrate in biofuel industry.
format	Thesis
qualification_level	Master's degree
author	Nur Syazana, Muhamad Nasrah
author_facet	Nur Syazana, Muhamad Nasrah
author_sort	Nur Syazana, Muhamad Nasrah
title	Biosynthesis of biobutanol from oil palm frond juice by Clostridium acetobutylicum
title_short	Biosynthesis of biobutanol from oil palm frond juice by Clostridium acetobutylicum
title_full	Biosynthesis of biobutanol from oil palm frond juice by Clostridium acetobutylicum
title_fullStr	Biosynthesis of biobutanol from oil palm frond juice by Clostridium acetobutylicum
title_full_unstemmed	Biosynthesis of biobutanol from oil palm frond juice by Clostridium acetobutylicum
title_sort	biosynthesis of biobutanol from oil palm frond juice by clostridium acetobutylicum
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Chemical and Process Engineering Technology
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/31112/1/Biosynthesis%20of%20biobutanol%20from%20oil%20palm%20frond%20juice%20by%20clostridium%20acetobutylicum.wm.pdf
_version_	1783732154754138112

Biosynthesis of biobutanol from oil palm frond juice by Clostridium acetobutylicum

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