Reverse micelle extraction of erythromycin with mixed surfactant anionic and zwitterionic

Most of the studies on reverse micelle extraction have been performed by using single anionic surfactant bis(2-ethylhexyl) sulfosuccinate sodium salt (AOT). However, the bio-molecules hosted in AOT reverse micelle were reported to be negatively affected by strong electrostatic interactions and high...

Full description

Saved in:

Bibliographic Details
Main Author:	Siti Norazimah, Mohamad Aziz
Format:	Thesis
Language:	English
Published:	2019
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/31116/1/Reverse%20micelle%20extraction%20of%20erythromycin%20with%20mixed%20surfactant%20anionic.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-ump-ir.31116
record_format	uketd_dc
spelling	my-ump-ir.311162021-04-12T08:26:03Z Reverse micelle extraction of erythromycin with mixed surfactant anionic and zwitterionic 2019-11 Siti Norazimah, Mohamad Aziz TP Chemical technology Most of the studies on reverse micelle extraction have been performed by using single anionic surfactant bis(2-ethylhexyl) sulfosuccinate sodium salt (AOT). However, the bio-molecules hosted in AOT reverse micelle were reported to be negatively affected by strong electrostatic interactions and high consumption of surfactant needed. Therefore, the addition of zwitterionic SB3-12 surfactant to the AOT reverse micellar was proposed for the extraction of erythromycin. This study aimed to observe the stability of mixed micelle formed based on the thermodynamic parameters which can be a potential method for antibiotic extraction. The kinetic parameter in the forward transfer was investigated, where the mass transfer kinetic, adsorptions mechanisms as well as optimum erythromycin extracted were determined. The experimental data were analyzed using Two-film theory, Langmuir, Freundlich and Sips isotherm model and the best fitted isotherm model was then determined using error analysis. For the optimization of forward extraction, a systematic experimental design including One-factor-at-time (OFAT) and full factorial design was used in the initial screening process to determine the significant variables factors. The optimized condition in forward extraction was further used in backward extraction. The factor effecting of backward extraction and kinetic mass transfer during recovery have been investigated. The CMCmix show a lower value in the range of 0.7-5.7 g/L suggests a superior surface activity compared to single surfactant. The negative value of ΔGm, ΔGads and ΔGoex indicate that the adsorption process was spontaneous and mixed reverse micelle formed was thermodynamically stable. The result proved that the addition of SB3-12 increase the stability of mixed micelle formed which provided a better microenvironment for bio-molecules. The two-film theory is appropriate for the mass transfer kinetic of erythromycin in forward extraction and the mass transfer kinetic was found to be controlled by interface solubilisation and the diffusion of the erythromycin in the aqueous phase boundary layer. The best fitting isotherm for erythromycin transfer was Langmuir isotherm, which is demonstrated by the highest values of coefficient of determination and was confirmed by three types of error analysis. The results of full factorial design (FFD) indicated that the AOT concentration, zwitterion fraction and pH of the aqueous phase, are the significant factors in forward extraction. The optimum values obtained for the forward transfers were AOT concentration, 80.7 g/L; zwitterion fraction, 0.24; and pH of aqueous, 4.7 with the optimum erythromycin transfer at 95.70 % was attained. It can be concluded that the highest erythromycin solubilisation was successfully obtained even with a low AOT surfactant which reflects the synergy between AOT and SB3-12. For the backward extraction, the extraction rates generally two orders slower than forward extraction, however, equilibrium time was found to be faster than the conventional method previously reported. The backward optimum conditions namely isopropanol v/v 3.9%; NaCl concentration, 26.5 g/L; and pH of aqueous 8.4 fulfill the conditions to successfully obtain a higher erythromycin recovery (95.01%). In backward extraction, pH was found to be significant which promotes backward extraction since erythromycin was easily to release from the mixed reverse. The anionic erythromycin predominates at higher pH, causes repulsion with the surfactant which promotes backward transfer. 2019-11 Thesis http://umpir.ump.edu.my/id/eprint/31116/ http://umpir.ump.edu.my/id/eprint/31116/1/Reverse%20micelle%20extraction%20of%20erythromycin%20with%20mixed%20surfactant%20anionic.pdf pdf en public phd doctoral Universiti Malaysia Pahang Faculty of Chemical and Process Engineering Technology
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Siti Norazimah, Mohamad Aziz Reverse micelle extraction of erythromycin with mixed surfactant anionic and zwitterionic
description	Most of the studies on reverse micelle extraction have been performed by using single anionic surfactant bis(2-ethylhexyl) sulfosuccinate sodium salt (AOT). However, the bio-molecules hosted in AOT reverse micelle were reported to be negatively affected by strong electrostatic interactions and high consumption of surfactant needed. Therefore, the addition of zwitterionic SB3-12 surfactant to the AOT reverse micellar was proposed for the extraction of erythromycin. This study aimed to observe the stability of mixed micelle formed based on the thermodynamic parameters which can be a potential method for antibiotic extraction. The kinetic parameter in the forward transfer was investigated, where the mass transfer kinetic, adsorptions mechanisms as well as optimum erythromycin extracted were determined. The experimental data were analyzed using Two-film theory, Langmuir, Freundlich and Sips isotherm model and the best fitted isotherm model was then determined using error analysis. For the optimization of forward extraction, a systematic experimental design including One-factor-at-time (OFAT) and full factorial design was used in the initial screening process to determine the significant variables factors. The optimized condition in forward extraction was further used in backward extraction. The factor effecting of backward extraction and kinetic mass transfer during recovery have been investigated. The CMCmix show a lower value in the range of 0.7-5.7 g/L suggests a superior surface activity compared to single surfactant. The negative value of ΔGm, ΔGads and ΔGoex indicate that the adsorption process was spontaneous and mixed reverse micelle formed was thermodynamically stable. The result proved that the addition of SB3-12 increase the stability of mixed micelle formed which provided a better microenvironment for bio-molecules. The two-film theory is appropriate for the mass transfer kinetic of erythromycin in forward extraction and the mass transfer kinetic was found to be controlled by interface solubilisation and the diffusion of the erythromycin in the aqueous phase boundary layer. The best fitting isotherm for erythromycin transfer was Langmuir isotherm, which is demonstrated by the highest values of coefficient of determination and was confirmed by three types of error analysis. The results of full factorial design (FFD) indicated that the AOT concentration, zwitterion fraction and pH of the aqueous phase, are the significant factors in forward extraction. The optimum values obtained for the forward transfers were AOT concentration, 80.7 g/L; zwitterion fraction, 0.24; and pH of aqueous, 4.7 with the optimum erythromycin transfer at 95.70 % was attained. It can be concluded that the highest erythromycin solubilisation was successfully obtained even with a low AOT surfactant which reflects the synergy between AOT and SB3-12. For the backward extraction, the extraction rates generally two orders slower than forward extraction, however, equilibrium time was found to be faster than the conventional method previously reported. The backward optimum conditions namely isopropanol v/v 3.9%; NaCl concentration, 26.5 g/L; and pH of aqueous 8.4 fulfill the conditions to successfully obtain a higher erythromycin recovery (95.01%). In backward extraction, pH was found to be significant which promotes backward extraction since erythromycin was easily to release from the mixed reverse. The anionic erythromycin predominates at higher pH, causes repulsion with the surfactant which promotes backward transfer.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Siti Norazimah, Mohamad Aziz
author_facet	Siti Norazimah, Mohamad Aziz
author_sort	Siti Norazimah, Mohamad Aziz
title	Reverse micelle extraction of erythromycin with mixed surfactant anionic and zwitterionic
title_short	Reverse micelle extraction of erythromycin with mixed surfactant anionic and zwitterionic
title_full	Reverse micelle extraction of erythromycin with mixed surfactant anionic and zwitterionic
title_fullStr	Reverse micelle extraction of erythromycin with mixed surfactant anionic and zwitterionic
title_full_unstemmed	Reverse micelle extraction of erythromycin with mixed surfactant anionic and zwitterionic
title_sort	reverse micelle extraction of erythromycin with mixed surfactant anionic and zwitterionic
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Chemical and Process Engineering Technology
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/31116/1/Reverse%20micelle%20extraction%20of%20erythromycin%20with%20mixed%20surfactant%20anionic.pdf
_version_	1783732155939028992

Reverse micelle extraction of erythromycin with mixed surfactant anionic and zwitterionic

Similar Items