Studies on local mango- and noniderived milk curds and their biological activities

The dairy market has grown favourably with constant growth rates in Malaysia in the past years. However, certain controversies surrounding the use of calf rennet as milk coagulant has encouraged the search for its alternatives, especially of plant origins. Hence, the study aims to investigate local...

وصف كامل

محفوظ في:

التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Norliana, Munir
التنسيق:	أطروحة
اللغة:	English
منشور في:	2021
الموضوعات:	HD Industries Land use Labor T Technology (General)
الوصول للمادة أونلاين:	http://umpir.ump.edu.my/id/eprint/35261/1/Studies%20on%20local%20mango-%20and%20noniderived%20milk%20curds%20and%20their%20biological%20activities.ir.pdf
الوسوم:	إضافة وسم لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!

id	my-ump-ir.35261
record_format	uketd_dc
spelling	my-ump-ir.352612022-10-14T02:05:45Z Studies on local mango- and noniderived milk curds and their biological activities 2021-02 Norliana, Munir HD Industries. Land use. Labor T Technology (General) The dairy market has grown favourably with constant growth rates in Malaysia in the past years. However, certain controversies surrounding the use of calf rennet as milk coagulant has encouraged the search for its alternatives, especially of plant origins. Hence, the study aims to investigate local plants having a dual effect of coagulating and fortifying milk simultaneously. In this study, ten samples from nine plants have been screened for the best two potential rapid milk coagulant. A total of three plants exhibited positive results on milk coagulation, with M. citrifolia juice extract showed the fastest coagulation time (10.8 ± 0.36 s) followed by M. indica seed kernel extract (28.8 ± 0.61 s) and T. catappa leaf extract (40.73 ± 1.91 s). Two plants proceeded with the further tests of determining the coagulant action by investigating the protein presence within extract through size exclusion chromatography, Bradford protein assay, SDS-PAGE analysis, and LCMS analysis. Both plants were proven to coagulate milk by enzyme action, with M. citrifolia was having definite proof of possessing protease-like enzyme (cysteine protease) with a molecular size of approximately 30 kDa. Then, the milk curd derived from both extracts of M. indica and M. citrifolia were further tested on their antioxidant and antidiabetic properties. The assays of TPC, DPPH, and FRAP were performed in determining the antioxidant activity of the milk curd. The result showed M. indica-added milk curd was having higher total phenolic content (34.7 ± 0.49 mg GAE/g) and antioxidant capacity of FRAP assay (317.16 ± 0.99 mg GAE/g), and the lower IC50 value for DPPH assay (12.31 ± 0.66 µg/mL) compared to M. citrifolia-added milk curd. Antidiabetic properties were assessed via α-amylase and α-glucosidase in vitro inhibitory activity assays. M. indica-added milk curd shown to have lower IC50 value in both assays compared to M. citrifolia-added milk curd, with 354.6 ± 20.26 µg/mL for α-amylase inhibitory assay and 4.86 ± 0.16 mg/mL for α-glucosidase inhibitory assay. Based on overall evaluations, M. indica was a better coagulant and fortifier of biological activities than M. citrifolia, and both plants found with the added advantage of being a fortifier compared to the rennet of only able to coagulate milk. The study showed the potential of local plants as milk coagulants as well as having benefits of biological activities and if developed into yogurt or cheese products, able to encourage the local dairy industry. 2021-02 Thesis http://umpir.ump.edu.my/id/eprint/35261/ http://umpir.ump.edu.my/id/eprint/35261/1/Studies%20on%20local%20mango-%20and%20noniderived%20milk%20curds%20and%20their%20biological%20activities.ir.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Industrial Sciences and Technology
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	HD Industries Land use Labor T Technology (General)
spellingShingle	HD Industries Land use Labor T Technology (General) Norliana, Munir Studies on local mango- and noniderived milk curds and their biological activities
description	The dairy market has grown favourably with constant growth rates in Malaysia in the past years. However, certain controversies surrounding the use of calf rennet as milk coagulant has encouraged the search for its alternatives, especially of plant origins. Hence, the study aims to investigate local plants having a dual effect of coagulating and fortifying milk simultaneously. In this study, ten samples from nine plants have been screened for the best two potential rapid milk coagulant. A total of three plants exhibited positive results on milk coagulation, with M. citrifolia juice extract showed the fastest coagulation time (10.8 ± 0.36 s) followed by M. indica seed kernel extract (28.8 ± 0.61 s) and T. catappa leaf extract (40.73 ± 1.91 s). Two plants proceeded with the further tests of determining the coagulant action by investigating the protein presence within extract through size exclusion chromatography, Bradford protein assay, SDS-PAGE analysis, and LCMS analysis. Both plants were proven to coagulate milk by enzyme action, with M. citrifolia was having definite proof of possessing protease-like enzyme (cysteine protease) with a molecular size of approximately 30 kDa. Then, the milk curd derived from both extracts of M. indica and M. citrifolia were further tested on their antioxidant and antidiabetic properties. The assays of TPC, DPPH, and FRAP were performed in determining the antioxidant activity of the milk curd. The result showed M. indica-added milk curd was having higher total phenolic content (34.7 ± 0.49 mg GAE/g) and antioxidant capacity of FRAP assay (317.16 ± 0.99 mg GAE/g), and the lower IC50 value for DPPH assay (12.31 ± 0.66 µg/mL) compared to M. citrifolia-added milk curd. Antidiabetic properties were assessed via α-amylase and α-glucosidase in vitro inhibitory activity assays. M. indica-added milk curd shown to have lower IC50 value in both assays compared to M. citrifolia-added milk curd, with 354.6 ± 20.26 µg/mL for α-amylase inhibitory assay and 4.86 ± 0.16 mg/mL for α-glucosidase inhibitory assay. Based on overall evaluations, M. indica was a better coagulant and fortifier of biological activities than M. citrifolia, and both plants found with the added advantage of being a fortifier compared to the rennet of only able to coagulate milk. The study showed the potential of local plants as milk coagulants as well as having benefits of biological activities and if developed into yogurt or cheese products, able to encourage the local dairy industry.
format	Thesis
qualification_level	Master's degree
author	Norliana, Munir
author_facet	Norliana, Munir
author_sort	Norliana, Munir
title	Studies on local mango- and noniderived milk curds and their biological activities
title_short	Studies on local mango- and noniderived milk curds and their biological activities
title_full	Studies on local mango- and noniderived milk curds and their biological activities
title_fullStr	Studies on local mango- and noniderived milk curds and their biological activities
title_full_unstemmed	Studies on local mango- and noniderived milk curds and their biological activities
title_sort	studies on local mango- and noniderived milk curds and their biological activities
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Industrial Sciences and Technology
publishDate	2021
url	http://umpir.ump.edu.my/id/eprint/35261/1/Studies%20on%20local%20mango-%20and%20noniderived%20milk%20curds%20and%20their%20biological%20activities.ir.pdf
_version_	1783732227064987648

Studies on local mango- and noniderived milk curds and their biological activities

مواد مشابهة