Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357

Monascus sp. produce useful Monascus pigments that widely used in food industry. Extensive study available on Monascus sp. in solid-state fermentation (SSF) using shake flask, however, cultivation of Monascus sp. in stirred drum bioreactor is insufficiently reported. Thus, this study aims to optimiz...

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Main Author:	Nur Fathin Shamirah, Daud
Format:	Thesis
Language:	English
Published:	2021
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/35302/1/Optimization%20and%20kinetic%20study%20of%20pigments%20production%20on%20solid%20state%20fermentation%20using%20oil%20palm%20frond.ir.pdf
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spelling	my-ump-ir.353022022-10-14T01:55:03Z Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357 2021-09 Nur Fathin Shamirah, Daud QD Chemistry TA Engineering (General). Civil engineering (General) Monascus sp. produce useful Monascus pigments that widely used in food industry. Extensive study available on Monascus sp. in solid-state fermentation (SSF) using shake flask, however, cultivation of Monascus sp. in stirred drum bioreactor is insufficiently reported. Thus, this study aims to optimize pigments production (i.e. yellow, orange and red) from Monascus purpureus FTC 5357 using oil palm frond (OPF) in 5 L stirred-drum bioreactor. Prior to the SSF process, hydrothermal treatment (121 °C, 15 min) was done. A three-level Box–Behnken design (BBD) was used for the optimization, with threeparameters such as initial moisture content (IMC), aeration rate (AR) and peptone concentration (PC). The dried fermented OPF were extracted and analysed for pigments production (i.e. yellow, orange and red), biomass and glucose concentration. Next, the kinetic profile of the optimize condition and kinetic parameters were also been evaluated. Based on the ANOVA analysis results, IMC, AR and PC contributed significantly to the pigments production. The optimal fermentation conditions resulted IMC at 70 % w/w, AR at 1.30 vvm and PC at 4.40 % w/w. Under these conditions, the pigments production were obtained at 22.03, 20.11 and 18.59 Au/g.d for yellow, orange and red, respectively. Validation experiment was done to confirm the adequacy of the models. The fermentation profile at optimized condition indicated that Monascus purpureus FTC 5357 reached maximum pigments and biomass at day 9. Results obtained from this study showed the importance of IMC, AR and PC for pigments production and biomass. Meanwhile, the production kinetic parameters such as maximum specific growth rate, the growth yield, pigment yield, cell productivity, pigments productivity for yellow, orange and red were 0.707 day-1, 0.15 mg/mg, 0.193 Au/mg cell and 12.514 mg/(g.day), 2.447 Au/(g.d.day), 2.234 Au/(g.d.day) and 2.065 Au/(g.d.day), respectively. The pigments produced through SSF process using OPF as a substrate by Monascus purpureus FTC 5357 has a great potential to be utilized as a source of pigment for food in future. 2021-09 Thesis http://umpir.ump.edu.my/id/eprint/35302/ http://umpir.ump.edu.my/id/eprint/35302/1/Optimization%20and%20kinetic%20study%20of%20pigments%20production%20on%20solid%20state%20fermentation%20using%20oil%20palm%20frond.ir.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Chemical and Process Engineering Technology
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	QD Chemistry QD Chemistry
spellingShingle	QD Chemistry QD Chemistry Nur Fathin Shamirah, Daud Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357
description	Monascus sp. produce useful Monascus pigments that widely used in food industry. Extensive study available on Monascus sp. in solid-state fermentation (SSF) using shake flask, however, cultivation of Monascus sp. in stirred drum bioreactor is insufficiently reported. Thus, this study aims to optimize pigments production (i.e. yellow, orange and red) from Monascus purpureus FTC 5357 using oil palm frond (OPF) in 5 L stirred-drum bioreactor. Prior to the SSF process, hydrothermal treatment (121 °C, 15 min) was done. A three-level Box–Behnken design (BBD) was used for the optimization, with threeparameters such as initial moisture content (IMC), aeration rate (AR) and peptone concentration (PC). The dried fermented OPF were extracted and analysed for pigments production (i.e. yellow, orange and red), biomass and glucose concentration. Next, the kinetic profile of the optimize condition and kinetic parameters were also been evaluated. Based on the ANOVA analysis results, IMC, AR and PC contributed significantly to the pigments production. The optimal fermentation conditions resulted IMC at 70 % w/w, AR at 1.30 vvm and PC at 4.40 % w/w. Under these conditions, the pigments production were obtained at 22.03, 20.11 and 18.59 Au/g.d for yellow, orange and red, respectively. Validation experiment was done to confirm the adequacy of the models. The fermentation profile at optimized condition indicated that Monascus purpureus FTC 5357 reached maximum pigments and biomass at day 9. Results obtained from this study showed the importance of IMC, AR and PC for pigments production and biomass. Meanwhile, the production kinetic parameters such as maximum specific growth rate, the growth yield, pigment yield, cell productivity, pigments productivity for yellow, orange and red were 0.707 day-1, 0.15 mg/mg, 0.193 Au/mg cell and 12.514 mg/(g.day), 2.447 Au/(g.d.day), 2.234 Au/(g.d.day) and 2.065 Au/(g.d.day), respectively. The pigments produced through SSF process using OPF as a substrate by Monascus purpureus FTC 5357 has a great potential to be utilized as a source of pigment for food in future.
format	Thesis
qualification_level	Master's degree
author	Nur Fathin Shamirah, Daud
author_facet	Nur Fathin Shamirah, Daud
author_sort	Nur Fathin Shamirah, Daud
title	Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357
title_short	Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357
title_full	Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357
title_fullStr	Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357
title_full_unstemmed	Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357
title_sort	optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Chemical and Process Engineering Technology
publishDate	2021
url	http://umpir.ump.edu.my/id/eprint/35302/1/Optimization%20and%20kinetic%20study%20of%20pigments%20production%20on%20solid%20state%20fermentation%20using%20oil%20palm%20frond.ir.pdf
_version_	1783732232598323200

Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357

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