The effect of torbangun plant (Coleus amboinicus Lour) active substances on lactation in mice

The main purpose of this research is to investigate the effects of active ingredients in the Torbangun plant to the mice lactation milk glands production. Torbangun leaves were extracted using soxhlet. The quantitative analysis of the extracted Torbangun leaves were analyzed by Gas Chromatography-Ma...

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Main Author:	Risch, Awalludin
Format:	Thesis
Language:	English
Published:	2013
Subjects:	RS Pharmacy and materia medica TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/37234/1/The%20effect%20of%20torbangun%20plant%20%28Coleus%20amboinicus%20Lour%29%20active%20substances%20on%20lactation%20in%20mice.pdf
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spelling	my-ump-ir.372342023-03-09T02:30:10Z The effect of torbangun plant (Coleus amboinicus Lour) active substances on lactation in mice 2013-02 Risch, Awalludin RS Pharmacy and materia medica TP Chemical technology The main purpose of this research is to investigate the effects of active ingredients in the Torbangun plant to the mice lactation milk glands production. Torbangun leaves were extracted using soxhlet. The quantitative analysis of the extracted Torbangun leaves were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS).The Fourier Transform Infra Red spectroscopy (FTIR) was applied to examine the functional group of the extracted samples; antibiotic screening tests was performed by the paper disk method; total phenolic content, total flavonoid content and antioxidant activity were analyzed using UV Vis-Spectrophotometer; heavy metals content was determined using ICPMS; Clinical trials were carried out on 36 mice which consisted of six control mice and each six mice were treated with 1%, 5%, 10, 15% and 20% of extracted Torbangun leave and mammary glands of mice were observed by histological analysis. The results indicated that the compounds in extracted Torbangun leave were phenol (PHE), gamma sitosterol, campesterol, Isocholesteryl methyl ether, Octadecanoic acid, Stigmasterol, Benzoic acid, 1,2-Benzenediol, phenol, 3-methoxy-2,4,5-trimethyl and alpha-amyrin; the functional group of extracted Torbangun leave were alcohol, ether, carboxylic acids and esters, aromatic rings and alkenes; antibiotic families determined in extracted Torbangun leaves were penicillin, tetracycline, aminoglycoside and macrolide; total phenolic content, total flavonoid content and antioxidant activity were 206.087 mg/L, 82.814 mg/L and 3.68 mg/L, respectively and heavy metals content in extracted Torbangun leaves were As (0.1 ppm), Pb (2.39 ppm), Cd (0.07), Mg (23.38 ppm) and Cu (15.42 ppm). The results of clinical trials showed that the differences between control mice and mice consumed with extracted Torbangun leave (1%, 5%, and 10%) for milk production were not significant, while the differences of the control mice and mice consumed with extracted Torbangun leave (15%, control and 20%) for milk production were significant. The histology of mammary glands for mice treated with 1%, 5% and 10% of extracted Torbangun leave exhibited equivalent to control mice.. While the mice treated with 15% and 20% of extracted Torbangun leave showed a lot of milk in the lobules (mammary gland). In conclusion, the active substances in extracted Torbangun leave influenced the function of lactating mammary gland, which consequently may increase milk production and it was proven safe for human consumption. 2013-02 Thesis http://umpir.ump.edu.my/id/eprint/37234/ http://umpir.ump.edu.my/id/eprint/37234/1/The%20effect%20of%20torbangun%20plant%20%28Coleus%20amboinicus%20Lour%29%20active%20substances%20on%20lactation%20in%20mice.pdf pdf en public phd doctoral Universiti Malaysia Pahang Faculty of Chemical & Natural Resources Engineering Mimi Sakinah, Abdul Munaim
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
advisor	Mimi Sakinah, Abdul Munaim
topic	RS Pharmacy and materia medica TP Chemical technology
spellingShingle	RS Pharmacy and materia medica TP Chemical technology Risch, Awalludin The effect of torbangun plant (Coleus amboinicus Lour) active substances on lactation in mice
description	The main purpose of this research is to investigate the effects of active ingredients in the Torbangun plant to the mice lactation milk glands production. Torbangun leaves were extracted using soxhlet. The quantitative analysis of the extracted Torbangun leaves were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS).The Fourier Transform Infra Red spectroscopy (FTIR) was applied to examine the functional group of the extracted samples; antibiotic screening tests was performed by the paper disk method; total phenolic content, total flavonoid content and antioxidant activity were analyzed using UV Vis-Spectrophotometer; heavy metals content was determined using ICPMS; Clinical trials were carried out on 36 mice which consisted of six control mice and each six mice were treated with 1%, 5%, 10, 15% and 20% of extracted Torbangun leave and mammary glands of mice were observed by histological analysis. The results indicated that the compounds in extracted Torbangun leave were phenol (PHE), gamma sitosterol, campesterol, Isocholesteryl methyl ether, Octadecanoic acid, Stigmasterol, Benzoic acid, 1,2-Benzenediol, phenol, 3-methoxy-2,4,5-trimethyl and alpha-amyrin; the functional group of extracted Torbangun leave were alcohol, ether, carboxylic acids and esters, aromatic rings and alkenes; antibiotic families determined in extracted Torbangun leaves were penicillin, tetracycline, aminoglycoside and macrolide; total phenolic content, total flavonoid content and antioxidant activity were 206.087 mg/L, 82.814 mg/L and 3.68 mg/L, respectively and heavy metals content in extracted Torbangun leaves were As (0.1 ppm), Pb (2.39 ppm), Cd (0.07), Mg (23.38 ppm) and Cu (15.42 ppm). The results of clinical trials showed that the differences between control mice and mice consumed with extracted Torbangun leave (1%, 5%, and 10%) for milk production were not significant, while the differences of the control mice and mice consumed with extracted Torbangun leave (15%, control and 20%) for milk production were significant. The histology of mammary glands for mice treated with 1%, 5% and 10% of extracted Torbangun leave exhibited equivalent to control mice.. While the mice treated with 15% and 20% of extracted Torbangun leave showed a lot of milk in the lobules (mammary gland). In conclusion, the active substances in extracted Torbangun leave influenced the function of lactating mammary gland, which consequently may increase milk production and it was proven safe for human consumption.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Risch, Awalludin
author_facet	Risch, Awalludin
author_sort	Risch, Awalludin
title	The effect of torbangun plant (Coleus amboinicus Lour) active substances on lactation in mice
title_short	The effect of torbangun plant (Coleus amboinicus Lour) active substances on lactation in mice
title_full	The effect of torbangun plant (Coleus amboinicus Lour) active substances on lactation in mice
title_fullStr	The effect of torbangun plant (Coleus amboinicus Lour) active substances on lactation in mice
title_full_unstemmed	The effect of torbangun plant (Coleus amboinicus Lour) active substances on lactation in mice
title_sort	effect of torbangun plant (coleus amboinicus lour) active substances on lactation in mice
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Chemical & Natural Resources Engineering
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/37234/1/The%20effect%20of%20torbangun%20plant%20%28Coleus%20amboinicus%20Lour%29%20active%20substances%20on%20lactation%20in%20mice.pdf
_version_	1783732267435163648

The effect of torbangun plant (Coleus amboinicus Lour) active substances on lactation in mice

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