Efficient cultivation of Kluyveromyces Lactis in high cell density culture in fed-batch cultivation system

Kluyveromyces lactis is beneficial and well-known yeast due to its status of GRAS that has made the yeast as a vital microorganism for the subject of studies and also the applications of industry such as a possible source of single-cell protein with expected probiotic properties, oligonuci eotide-de...

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Main Author:	Mohd Shafiq, Mohd Sueb
Format:	Thesis
Language:	English
Published:	2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/4723/1/MOHD_SHAFIQ_B_MOHD_SUEB.PDF
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id	my-ump-ir.4723
record_format	uketd_dc
spelling	my-ump-ir.47232021-08-16T04:24:06Z Efficient cultivation of Kluyveromyces Lactis in high cell density culture in fed-batch cultivation system 2012-06 Mohd Shafiq, Mohd Sueb TP Chemical technology Kluyveromyces lactis is beneficial and well-known yeast due to its status of GRAS that has made the yeast as a vital microorganism for the subject of studies and also the applications of industry such as a possible source of single-cell protein with expected probiotic properties, oligonuci eotide-derived flavour enhancers and lactic acid. In addition,High Cell Density Cultivation (HCDC) of K. lactis has been scientifically and biotechnologically important trend in improving microbial mass and product formation substantially.More importantly,fed-batch strategy has been identified as another effective mean to increase the yield by preventing substrate limitation or inhibition through maintaining medium substrate concentration at a low level during cultivation.In this study,there were two system cultivations have been employed which was batch cultivation and fed-batch cultivation. Further studies have been done under batch cultivation on the aeration effect and dissolved The results showed that 1.0 v/v/inin aeration rate gave relatively high cell dry weight, CDW, 18.6 gIL. On the contrary, the results from DO stat value of 60% showed substantial increment with CDW of 28.7 gIL.As for the fed-batch study, constant feeding rate strategy has been applied with two different feeding substrates i.e complete media and mono-lactate. The CDW harvested for complete media had showed remarkable result, 59.75 g/L as compared to CDW produced from lactose as a sole feeding substrate, 44.75 g/L.02,DO. 2012-06 Thesis http://umpir.ump.edu.my/id/eprint/4723/ http://umpir.ump.edu.my/id/eprint/4723/1/MOHD_SHAFIQ_B_MOHD_SUEB.PDF application/pdf en public masters Universiti Teknologi Malaysia Faculty of Chemical Engineering
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Mohd Shafiq, Mohd Sueb Efficient cultivation of Kluyveromyces Lactis in high cell density culture in fed-batch cultivation system
description	Kluyveromyces lactis is beneficial and well-known yeast due to its status of GRAS that has made the yeast as a vital microorganism for the subject of studies and also the applications of industry such as a possible source of single-cell protein with expected probiotic properties, oligonuci eotide-derived flavour enhancers and lactic acid. In addition,High Cell Density Cultivation (HCDC) of K. lactis has been scientifically and biotechnologically important trend in improving microbial mass and product formation substantially.More importantly,fed-batch strategy has been identified as another effective mean to increase the yield by preventing substrate limitation or inhibition through maintaining medium substrate concentration at a low level during cultivation.In this study,there were two system cultivations have been employed which was batch cultivation and fed-batch cultivation. Further studies have been done under batch cultivation on the aeration effect and dissolved The results showed that 1.0 v/v/inin aeration rate gave relatively high cell dry weight, CDW, 18.6 gIL. On the contrary, the results from DO stat value of 60% showed substantial increment with CDW of 28.7 gIL.As for the fed-batch study, constant feeding rate strategy has been applied with two different feeding substrates i.e complete media and mono-lactate. The CDW harvested for complete media had showed remarkable result, 59.75 g/L as compared to CDW produced from lactose as a sole feeding substrate, 44.75 g/L.02,DO.
format	Thesis
qualification_level	Master's degree
author	Mohd Shafiq, Mohd Sueb
author_facet	Mohd Shafiq, Mohd Sueb
author_sort	Mohd Shafiq, Mohd Sueb
title	Efficient cultivation of Kluyveromyces Lactis in high cell density culture in fed-batch cultivation system
title_short	Efficient cultivation of Kluyveromyces Lactis in high cell density culture in fed-batch cultivation system
title_full	Efficient cultivation of Kluyveromyces Lactis in high cell density culture in fed-batch cultivation system
title_fullStr	Efficient cultivation of Kluyveromyces Lactis in high cell density culture in fed-batch cultivation system
title_full_unstemmed	Efficient cultivation of Kluyveromyces Lactis in high cell density culture in fed-batch cultivation system
title_sort	efficient cultivation of kluyveromyces lactis in high cell density culture in fed-batch cultivation system
granting_institution	Universiti Teknologi Malaysia
granting_department	Faculty of Chemical Engineering
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/4723/1/MOHD_SHAFIQ_B_MOHD_SUEB.PDF
_version_	1783731911079755776

Efficient cultivation of Kluyveromyces Lactis in high cell density culture in fed-batch cultivation system

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