Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source

The increasing non-biodegradable plastic waste materials have created critical need in finding a better replacement to the currently available conventional plastic. Researches have shown great interest in the production of polyhydroxyalkanoate (PHA) biopolymer from bacterial fermentation. Although s...

Full description

Saved in:

Bibliographic Details
Main Author:	Abeed Fatima, Mohidin Batcha
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/9467/1/Biosynthesis%20of%20poly%20%283-hydroxybutyrate%29%20%28PHB%29%20by%20cupriavidus%20necator%20H16%20from%20jatropha%20oil%20as%20carbon%20source.%20Masters%20thesis%2C%20Universiti%20Malaysia%20Pahang.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-ump-ir.9467
record_format	uketd_dc
spelling	my-ump-ir.94672023-05-09T07:23:56Z Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source 2014-02 Abeed Fatima, Mohidin Batcha QD Chemistry The increasing non-biodegradable plastic waste materials have created critical need in finding a better replacement to the currently available conventional plastic. Researches have shown great interest in the production of polyhydroxyalkanoate (PHA) biopolymer from bacterial fermentation. Although successful attempts have been made in producing these short to medium-chain length biopolymers, there are still problems in terms of yield and cost effectiveness that needs to be resolved. Poly(3-hydroxybutyrate) (PHB), a homopolymer of PHA is one particular example of bioplastic that are naturally produced by bacteria like Cupriavidus necator sp. By using plant oils such as jatropha oil as an alternative, a higher yield of PHB can be obtained and thus reducing the overall production cost of the biopolymers. In this study, Cupriavidus necator H16 was used to synthesize PHB by using jatropha oil as its sole carbon source. Different variables mainly jatropha oil and urea concentrations, and agitation speed were investigated to determine the optimum condition for microbial fermentation in batch culture. Based on the results, the highest cell dry weight and PHB concentration of 20.1 g/L and 15.5 g/L respectively was obtained when 20 g/L of jatropha oil was used along with 1 g/L of urea at 200 rpm of agitation speed. Ethanol was used as external stress factor and the addition of 1.5% (v/v) ethanol at 38 h had a positive effect with a high PHB yield of 0.987 g PHB/g jatropha oil. The kinetic studies for cell growth rate and PHB production were conducted and the data were fitted with Logistic and Leudeking Piret models. The rate constants were evaluated and the theoretical values were in accordance with the experimental data obtained. Optimization through Response Surface Methodology (RSM) at the condition of 0.9 g/L urea, 23.6 g/L jatropha oil and 251 rpm agitation speed resulted in 5% increase in PHB concentration to 17.92 g/L compared to the previously obtained PHB concentration of 17.05 g/L. The present work has succeeded in obtaining a high yield of PHB from an inexpensive raw material. 2014-02 Thesis http://umpir.ump.edu.my/id/eprint/9467/ http://umpir.ump.edu.my/id/eprint/9467/1/Biosynthesis%20of%20poly%20%283-hydroxybutyrate%29%20%28PHB%29%20by%20cupriavidus%20necator%20H16%20from%20jatropha%20oil%20as%20carbon%20source.%20Masters%20thesis%2C%20Universiti%20Malaysia%20Pahang.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Chemical and Natural Resources Engineering Rahman Khan, Md. Maksudur
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
advisor	Rahman Khan, Md. Maksudur
topic	QD Chemistry
spellingShingle	QD Chemistry Abeed Fatima, Mohidin Batcha Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
description	The increasing non-biodegradable plastic waste materials have created critical need in finding a better replacement to the currently available conventional plastic. Researches have shown great interest in the production of polyhydroxyalkanoate (PHA) biopolymer from bacterial fermentation. Although successful attempts have been made in producing these short to medium-chain length biopolymers, there are still problems in terms of yield and cost effectiveness that needs to be resolved. Poly(3-hydroxybutyrate) (PHB), a homopolymer of PHA is one particular example of bioplastic that are naturally produced by bacteria like Cupriavidus necator sp. By using plant oils such as jatropha oil as an alternative, a higher yield of PHB can be obtained and thus reducing the overall production cost of the biopolymers. In this study, Cupriavidus necator H16 was used to synthesize PHB by using jatropha oil as its sole carbon source. Different variables mainly jatropha oil and urea concentrations, and agitation speed were investigated to determine the optimum condition for microbial fermentation in batch culture. Based on the results, the highest cell dry weight and PHB concentration of 20.1 g/L and 15.5 g/L respectively was obtained when 20 g/L of jatropha oil was used along with 1 g/L of urea at 200 rpm of agitation speed. Ethanol was used as external stress factor and the addition of 1.5% (v/v) ethanol at 38 h had a positive effect with a high PHB yield of 0.987 g PHB/g jatropha oil. The kinetic studies for cell growth rate and PHB production were conducted and the data were fitted with Logistic and Leudeking Piret models. The rate constants were evaluated and the theoretical values were in accordance with the experimental data obtained. Optimization through Response Surface Methodology (RSM) at the condition of 0.9 g/L urea, 23.6 g/L jatropha oil and 251 rpm agitation speed resulted in 5% increase in PHB concentration to 17.92 g/L compared to the previously obtained PHB concentration of 17.05 g/L. The present work has succeeded in obtaining a high yield of PHB from an inexpensive raw material.
format	Thesis
qualification_level	Master's degree
author	Abeed Fatima, Mohidin Batcha
author_facet	Abeed Fatima, Mohidin Batcha
author_sort	Abeed Fatima, Mohidin Batcha
title	Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
title_short	Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
title_full	Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
title_fullStr	Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
title_full_unstemmed	Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
title_sort	biosynthesis of poly (3-hydroxybutyrate) (phb) by cupriavidus necator h16 from jatropha oil as carbon source
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Chemical and Natural Resources Engineering
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/9467/1/Biosynthesis%20of%20poly%20%283-hydroxybutyrate%29%20%28PHB%29%20by%20cupriavidus%20necator%20H16%20from%20jatropha%20oil%20as%20carbon%20source.%20Masters%20thesis%2C%20Universiti%20Malaysia%20Pahang.pdf
_version_	1783731954844172288

Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source

Similar Items