Efficacy of antimicrobial compounds in Camellia sinensis for red root pathogen

Red root disease of tea is a primary root disease in Sabah Tea Plantation. The causal pathogen was successfully isolated and was identified as Schizopora flavipora, which previously known as Poria hypolateritia. The crude ethanolic extracts of tea ( Camellia sinensis var. assamica) seed, root, stem...

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Main Author:	Chin, Clament Fui Seung
Format:	Thesis
Language:	English English
Published:	2010
Subjects:	SB183-317 Field crops Including cereals forage crops grasses legumes root crops sugar plants textile plants alkaloidal plants medicinal plants
Online Access:	https://eprints.ums.edu.my/id/eprint/10109/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/10109/2/FULLTEXT.pdf
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spelling	my-ums-ep.101092024-04-15T07:13:39Z Efficacy of antimicrobial compounds in Camellia sinensis for red root pathogen 2010 Chin, Clament Fui Seung SB183-317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants Red root disease of tea is a primary root disease in Sabah Tea Plantation. The causal pathogen was successfully isolated and was identified as Schizopora flavipora, which previously known as Poria hypolateritia. The crude ethanolic extracts of tea ( Camellia sinensis var. assamica) seed, root, stem and leaf; as well as their correspondence fractions (hexane, dichloromethane, ethyl acetate, butanol, and aqueous) were screened in vitro for antifungal activities using disc diffusion technique. Extracts from tea leaf and young immature green stem were more fungistatic than the root and seed extracts. Hexane, dichloromethane and ethyl acetate fractions obtained from the ethanolic leaf extracts by liquid-liquid extractions were more effective than the butanol or aqueous fraction when tested against the pathogen. The compounds that are responsible for fungitoxicity were identified by thin layer chromatography and high performance chromatography to be essential oils, alkaloids, phenolic acids and catechins. The constituents of essential oil that are responsible for fungitoxicity were identified to be geraniol, nerol, nerolidol, linalool, linalool oxide and benzyl acetate by the vanillin-sulphuric acid reagent, with the first two being the more potential, having a minimum inhibitory concentration (MIC) value of 0.25 mg.ml-1 . The groups of non-volatile active compounds were identified as caffeine, gallic acid, catechins and gallic acid esterified catechins, with the first two having the lowest MIC value (0.10 mg.mL-1). The general distribution pattern of the non-volatile active compounds on thirteen parts of the tea plant were further determined and were found to contain highest in leaf bud and lowest in old root cortex. In this study, neither phytoalexins production nor active compounds accumulation was recorded after elicitation treatment using 0.1 % (w/v) chitosan on leaves and roots of two years old tea seedlings. The study demonstrated that leaf extracts of C sinensis exhibit strong fungitoxicity against Schizopora f/avipora, and have potential to be used as mulching materials in infested soils through integrated pest management (IPM) strategy in organic farming, as well as formulating into biopesticide product for the control of red root disease in conventional farming. 2010 Thesis https://eprints.ums.edu.my/id/eprint/10109/ https://eprints.ums.edu.my/id/eprint/10109/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/10109/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah School of Sustainable Agriculture
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	SB183-317 Field crops Including cereals forage crops grasses legumes root crops sugar plants textile plants alkaloidal plants medicinal plants
spellingShingle	SB183-317 Field crops Including cereals forage crops grasses legumes root crops sugar plants textile plants alkaloidal plants medicinal plants Chin, Clament Fui Seung Efficacy of antimicrobial compounds in Camellia sinensis for red root pathogen
description	Red root disease of tea is a primary root disease in Sabah Tea Plantation. The causal pathogen was successfully isolated and was identified as Schizopora flavipora, which previously known as Poria hypolateritia. The crude ethanolic extracts of tea ( Camellia sinensis var. assamica) seed, root, stem and leaf; as well as their correspondence fractions (hexane, dichloromethane, ethyl acetate, butanol, and aqueous) were screened in vitro for antifungal activities using disc diffusion technique. Extracts from tea leaf and young immature green stem were more fungistatic than the root and seed extracts. Hexane, dichloromethane and ethyl acetate fractions obtained from the ethanolic leaf extracts by liquid-liquid extractions were more effective than the butanol or aqueous fraction when tested against the pathogen. The compounds that are responsible for fungitoxicity were identified by thin layer chromatography and high performance chromatography to be essential oils, alkaloids, phenolic acids and catechins. The constituents of essential oil that are responsible for fungitoxicity were identified to be geraniol, nerol, nerolidol, linalool, linalool oxide and benzyl acetate by the vanillin-sulphuric acid reagent, with the first two being the more potential, having a minimum inhibitory concentration (MIC) value of 0.25 mg.ml-1 . The groups of non-volatile active compounds were identified as caffeine, gallic acid, catechins and gallic acid esterified catechins, with the first two having the lowest MIC value (0.10 mg.mL-1). The general distribution pattern of the non-volatile active compounds on thirteen parts of the tea plant were further determined and were found to contain highest in leaf bud and lowest in old root cortex. In this study, neither phytoalexins production nor active compounds accumulation was recorded after elicitation treatment using 0.1 % (w/v) chitosan on leaves and roots of two years old tea seedlings. The study demonstrated that leaf extracts of C sinensis exhibit strong fungitoxicity against Schizopora f/avipora, and have potential to be used as mulching materials in infested soils through integrated pest management (IPM) strategy in organic farming, as well as formulating into biopesticide product for the control of red root disease in conventional farming.
format	Thesis
qualification_level	Master's degree
author	Chin, Clament Fui Seung
author_facet	Chin, Clament Fui Seung
author_sort	Chin, Clament Fui Seung
title	Efficacy of antimicrobial compounds in Camellia sinensis for red root pathogen
title_short	Efficacy of antimicrobial compounds in Camellia sinensis for red root pathogen
title_full	Efficacy of antimicrobial compounds in Camellia sinensis for red root pathogen
title_fullStr	Efficacy of antimicrobial compounds in Camellia sinensis for red root pathogen
title_full_unstemmed	Efficacy of antimicrobial compounds in Camellia sinensis for red root pathogen
title_sort	efficacy of antimicrobial compounds in camellia sinensis for red root pathogen
granting_institution	Universiti Malaysia Sabah
granting_department	School of Sustainable Agriculture
publishDate	2010
url	https://eprints.ums.edu.my/id/eprint/10109/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/10109/2/FULLTEXT.pdf
_version_	1804890310278381568

Efficacy of antimicrobial compounds in Camellia sinensis for red root pathogen

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