Association of CYP2E1 polymorphisms with gastrointestinal cancer in Malaysia

Malaysia has a high mortality rate of gastrointestinal cancer (GIC) and hence finding a biomarker for GIC is crucial. CYP2E1 polymorphisms have been associated to increase the risk of GIC in many populations but remain unclear in Malaysian. This study was aimed to investigate the association betw...

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Main Author:	Chong, Eric Tzvy Jiann
Format:	Thesis
Language:	English
Published:	2013
Subjects:	RC Internal medicine
Online Access:	https://eprints.ums.edu.my/id/eprint/17641/1/Association%20of%20CYP2E1%20polymorphisms.pdf
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spelling	my-ums-ep.176412017-11-23T04:42:56Z Association of CYP2E1 polymorphisms with gastrointestinal cancer in Malaysia 2013 Chong, Eric Tzvy Jiann RC Internal medicine Malaysia has a high mortality rate of gastrointestinal cancer (GIC) and hence finding a biomarker for GIC is crucial. CYP2E1 polymorphisms have been associated to increase the risk of GIC in many populations but remain unclear in Malaysian. This study was aimed to investigate the association between CYP2E1C -1019T and T7678A polymorphisms, as well as other risk factors such as age, ethnics, and gender to risk of GIC in Malaysia. DNA was extracted from 520 control and 175 GIC blood samples using alkaline lysis method, subjected to polymerase chain reaction followed by restriction fragment length polymorphism( PCR-RFLP)T. he SNPs were sequenced for confirmation. Cases and controls in both polymorphisms were found to be in Hardy-Weinberg equilibrium. In C-1019T SNP, the frequencies for c2 allele and c2/c2 genotype were 18.5% and 3.9%, respectively whereas the c2 allele and carrier with at least one c2 allele were found to significantly increase the risk to GIC by 1.38- and 1.45-fold, respectively. In contrast, the T7678A SNP revealed a frequency of 22.1% for C allele and 6.2% for C/C genotype in Malaysia. The T7678A SNP and combined C-1019T and T7678A SNPs did not contribute to GIC development in this study. Besides, elderly who aged >40 years old had a highly increased risk to GIC and Chinese showed greater risk to GIC when compared with the Malays, Indians and KadazanDusun but lower with other ethnic groups in Malaysia. It is worth to note that females were predominant to GIC and further investigation revealed that female had a 2.22- and 1.58-fold greater risk of gastric and colorectal cancers, respectively, when compared with males in Malaysia. In addition, the genotype distributions for both polymorphisms between Asian and non-Asian populations were significantly different. This study suggests that c2 allele and carrier with at least one c2 allele for C-1019T polymorphism may be used as a biomarker for GIC in Malaysia but further studies with larger sample size and including different gene-gene and gene-environmental interactions are needed 2013 Thesis https://eprints.ums.edu.my/id/eprint/17641/ https://eprints.ums.edu.my/id/eprint/17641/1/Association%20of%20CYP2E1%20polymorphisms.pdf text en public masters Universiti Malaysia Sabah Faculty of Science and Natural Resources
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English
topic	RC Internal medicine
spellingShingle	RC Internal medicine Chong, Eric Tzvy Jiann Association of CYP2E1 polymorphisms with gastrointestinal cancer in Malaysia
description	Malaysia has a high mortality rate of gastrointestinal cancer (GIC) and hence finding a biomarker for GIC is crucial. CYP2E1 polymorphisms have been associated to increase the risk of GIC in many populations but remain unclear in Malaysian. This study was aimed to investigate the association between CYP2E1C -1019T and T7678A polymorphisms, as well as other risk factors such as age, ethnics, and gender to risk of GIC in Malaysia. DNA was extracted from 520 control and 175 GIC blood samples using alkaline lysis method, subjected to polymerase chain reaction followed by restriction fragment length polymorphism( PCR-RFLP)T. he SNPs were sequenced for confirmation. Cases and controls in both polymorphisms were found to be in Hardy-Weinberg equilibrium. In C-1019T SNP, the frequencies for c2 allele and c2/c2 genotype were 18.5% and 3.9%, respectively whereas the c2 allele and carrier with at least one c2 allele were found to significantly increase the risk to GIC by 1.38- and 1.45-fold, respectively. In contrast, the T7678A SNP revealed a frequency of 22.1% for C allele and 6.2% for C/C genotype in Malaysia. The T7678A SNP and combined C-1019T and T7678A SNPs did not contribute to GIC development in this study. Besides, elderly who aged >40 years old had a highly increased risk to GIC and Chinese showed greater risk to GIC when compared with the Malays, Indians and KadazanDusun but lower with other ethnic groups in Malaysia. It is worth to note that females were predominant to GIC and further investigation revealed that female had a 2.22- and 1.58-fold greater risk of gastric and colorectal cancers, respectively, when compared with males in Malaysia. In addition, the genotype distributions for both polymorphisms between Asian and non-Asian populations were significantly different. This study suggests that c2 allele and carrier with at least one c2 allele for C-1019T polymorphism may be used as a biomarker for GIC in Malaysia but further studies with larger sample size and including different gene-gene and gene-environmental interactions are needed
format	Thesis
qualification_level	Master's degree
author	Chong, Eric Tzvy Jiann
author_facet	Chong, Eric Tzvy Jiann
author_sort	Chong, Eric Tzvy Jiann
title	Association of CYP2E1 polymorphisms with gastrointestinal cancer in Malaysia
title_short	Association of CYP2E1 polymorphisms with gastrointestinal cancer in Malaysia
title_full	Association of CYP2E1 polymorphisms with gastrointestinal cancer in Malaysia
title_fullStr	Association of CYP2E1 polymorphisms with gastrointestinal cancer in Malaysia
title_full_unstemmed	Association of CYP2E1 polymorphisms with gastrointestinal cancer in Malaysia
title_sort	association of cyp2e1 polymorphisms with gastrointestinal cancer in malaysia
granting_institution	Universiti Malaysia Sabah
granting_department	Faculty of Science and Natural Resources
publishDate	2013
url	https://eprints.ums.edu.my/id/eprint/17641/1/Association%20of%20CYP2E1%20polymorphisms.pdf
_version_	1747836457154772992

Association of CYP2E1 polymorphisms with gastrointestinal cancer in Malaysia

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