Screening of preliminary biological activities and in-vitro cultures Pereskia Bleo and Rennellia Elliptica
Peieskh bleo has been used by the local community of Malaysia to treat diabetes, hypertension, anti-tumor, anti-rheumatic, anti-ulcer and anti-inflammatory, while Rennellia elliptica were used to enhance inner strength, post natal treatment and to relief joints pain. Screening of phytochemicals,...
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| Format: | Thesis |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/17836/1/Screening%20of%20preliminary%20biological.pdf |
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| Summary: | Peieskh bleo has been used by the local community of Malaysia to treat diabetes,
hypertension, anti-tumor, anti-rheumatic, anti-ulcer and anti-inflammatory, while
Rennellia elliptica were used to enhance inner strength, post natal treatment and to
relief joints pain. Screening of phytochemicals, antibacterial, antifungal and inhibitor for
protein phosphatase type 1, MAP kinase and MAP kinase phosphatase were performed to
evaluate the medicinal properties of these plants. Bioassay through agar diffusion
method shows that moderately polar extracts (MPE) for both plants were biologically
active. The MPE of old leaves of P. bleo shows the best Inhibitory activities against S.
typhi (23.00±0.50 mm), protein phosphatase type 1 (14.17±1.26 mm), MAPK kinase
(33.33±1.44 mm), and MAPK kinase phosphatase (10.50±0.50 mm). The MPE of the
leaves of R. elliptica shows the largest inhibition on P. vulgaris (17.33±0.76 mm), while
the MPE of the stems shows the best inhibitory activities against MAPK kinase
(16.50±1.32 mm), and MAPK kinase phosphatase (9.33±0.58 mm). In vitro seeds
germination of P. bleo was 100% germination using Murashige and Skoog medium (MS),
B5 medium and Vacin and Went medium. Callus induction from the seed of P. bled using
MS medium supplemented with 2.5 mg/L 2,4-D and 1.5 mg/L kinetin give the highest
callus fresh weight (2.9833±0.1302 g). The root explants of R. elliptica formed callus on
MS medium supplemented with 0.4 mg/L to 1.0 mg/L 2,4-D. Roots induction from the
root-derived callus was accomplished on MS medium supplemented with 2.0 mg/L IBA
and 0.2 mg/L BAP. |
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