Screening of preliminary biological activities and in-vitro cultures Pereskia Bleo and Rennellia Elliptica

Peieskh bleo has been used by the local community of Malaysia to treat diabetes, hypertension, anti-tumor, anti-rheumatic, anti-ulcer and anti-inflammatory, while Rennellia elliptica were used to enhance inner strength, post natal treatment and to relief joints pain. Screening of phytochemicals,...

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Main Author:	Aina Syafiqah Abdullah
Format:	Thesis
Language:	English
Published:	2010
Subjects:	QD Chemistry
Online Access:	https://eprints.ums.edu.my/id/eprint/17836/1/Screening%20of%20preliminary%20biological.pdf
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id	my-ums-ep.17836
record_format	uketd_dc
spelling	my-ums-ep.178362017-12-28T22:35:06Z Screening of preliminary biological activities and in-vitro cultures Pereskia Bleo and Rennellia Elliptica 2010 Aina Syafiqah Abdullah QD Chemistry Peieskh bleo has been used by the local community of Malaysia to treat diabetes, hypertension, anti-tumor, anti-rheumatic, anti-ulcer and anti-inflammatory, while Rennellia elliptica were used to enhance inner strength, post natal treatment and to relief joints pain. Screening of phytochemicals, antibacterial, antifungal and inhibitor for protein phosphatase type 1, MAP kinase and MAP kinase phosphatase were performed to evaluate the medicinal properties of these plants. Bioassay through agar diffusion method shows that moderately polar extracts (MPE) for both plants were biologically active. The MPE of old leaves of P. bleo shows the best Inhibitory activities against S. typhi (23.00±0.50 mm), protein phosphatase type 1 (14.17±1.26 mm), MAPK kinase (33.33±1.44 mm), and MAPK kinase phosphatase (10.50±0.50 mm). The MPE of the leaves of R. elliptica shows the largest inhibition on P. vulgaris (17.33±0.76 mm), while the MPE of the stems shows the best inhibitory activities against MAPK kinase (16.50±1.32 mm), and MAPK kinase phosphatase (9.33±0.58 mm). In vitro seeds germination of P. bleo was 100% germination using Murashige and Skoog medium (MS), B5 medium and Vacin and Went medium. Callus induction from the seed of P. bled using MS medium supplemented with 2.5 mg/L 2,4-D and 1.5 mg/L kinetin give the highest callus fresh weight (2.9833±0.1302 g). The root explants of R. elliptica formed callus on MS medium supplemented with 0.4 mg/L to 1.0 mg/L 2,4-D. Roots induction from the root-derived callus was accomplished on MS medium supplemented with 2.0 mg/L IBA and 0.2 mg/L BAP. 2010 Thesis https://eprints.ums.edu.my/id/eprint/17836/ https://eprints.ums.edu.my/id/eprint/17836/1/Screening%20of%20preliminary%20biological.pdf text en public masters Universiti Malaysia Sabah Faculty of Science and Natural Resources
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Aina Syafiqah Abdullah Screening of preliminary biological activities and in-vitro cultures Pereskia Bleo and Rennellia Elliptica
description	Peieskh bleo has been used by the local community of Malaysia to treat diabetes, hypertension, anti-tumor, anti-rheumatic, anti-ulcer and anti-inflammatory, while Rennellia elliptica were used to enhance inner strength, post natal treatment and to relief joints pain. Screening of phytochemicals, antibacterial, antifungal and inhibitor for protein phosphatase type 1, MAP kinase and MAP kinase phosphatase were performed to evaluate the medicinal properties of these plants. Bioassay through agar diffusion method shows that moderately polar extracts (MPE) for both plants were biologically active. The MPE of old leaves of P. bleo shows the best Inhibitory activities against S. typhi (23.00±0.50 mm), protein phosphatase type 1 (14.17±1.26 mm), MAPK kinase (33.33±1.44 mm), and MAPK kinase phosphatase (10.50±0.50 mm). The MPE of the leaves of R. elliptica shows the largest inhibition on P. vulgaris (17.33±0.76 mm), while the MPE of the stems shows the best inhibitory activities against MAPK kinase (16.50±1.32 mm), and MAPK kinase phosphatase (9.33±0.58 mm). In vitro seeds germination of P. bleo was 100% germination using Murashige and Skoog medium (MS), B5 medium and Vacin and Went medium. Callus induction from the seed of P. bled using MS medium supplemented with 2.5 mg/L 2,4-D and 1.5 mg/L kinetin give the highest callus fresh weight (2.9833±0.1302 g). The root explants of R. elliptica formed callus on MS medium supplemented with 0.4 mg/L to 1.0 mg/L 2,4-D. Roots induction from the root-derived callus was accomplished on MS medium supplemented with 2.0 mg/L IBA and 0.2 mg/L BAP.
format	Thesis
qualification_level	Master's degree
author	Aina Syafiqah Abdullah
author_facet	Aina Syafiqah Abdullah
author_sort	Aina Syafiqah Abdullah
title	Screening of preliminary biological activities and in-vitro cultures Pereskia Bleo and Rennellia Elliptica
title_short	Screening of preliminary biological activities and in-vitro cultures Pereskia Bleo and Rennellia Elliptica
title_full	Screening of preliminary biological activities and in-vitro cultures Pereskia Bleo and Rennellia Elliptica
title_fullStr	Screening of preliminary biological activities and in-vitro cultures Pereskia Bleo and Rennellia Elliptica
title_full_unstemmed	Screening of preliminary biological activities and in-vitro cultures Pereskia Bleo and Rennellia Elliptica
title_sort	screening of preliminary biological activities and in-vitro cultures pereskia bleo and rennellia elliptica
granting_institution	Universiti Malaysia Sabah
granting_department	Faculty of Science and Natural Resources
publishDate	2010
url	https://eprints.ums.edu.my/id/eprint/17836/1/Screening%20of%20preliminary%20biological.pdf
_version_	1747836466249072640

Screening of preliminary biological activities and in-vitro cultures Pereskia Bleo and Rennellia Elliptica

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